32results about How to "Less sample required" patented technology

The invention belongs to the field of immune detection and analysis, and particularly relates to a full-automatic micro-fluidic chip fluorescence immune detection system and a detection method implemented by the same. The full-automatic micro-fluidic chip fluorescence immune detection system comprises a shell and a main framework, and is characterized in that a chip card storage disc and a sample tube disc are fixed to the upper portion of the main framework, a centrifugal reaction disc is arranged between the chip card storage disc and the sample tube disc, and a filling system and a cleaning system are arranged between the centrifugal reaction disc and the sample tube disc; an optical detection system is arranged on the main framework and corresponds to the centrifugal reaction disc, a control system and motors are arranged inside the main framework, and the chip card storage disc, the centrifugal reaction disc, the sample tube disc and the filling system can be driven by the motors to rotate. The full-automatic micro-fluidic chip fluorescence immune detection system and the detection method have the advantage that requirements on full automation, multiple samples, high flux and quick detection can be fundamentally met by the full-automatic micro-fluidic chip fluorescence immune detection system.

The invention discloses an electric vehicle power battery SOC intelligent detection device which is characterized in that the intelligent detection device includes a battery parameter acquisition platform and a battery SOC estimation system, the battery parameter acquisition platform collects real-time parameters of voltage, current and temperature of an electric vehicle power battery group, the battery SOC estimation system estimates an SOC value through the collected parameters, and a battery SOC system is a nonlinear, time-delay and multivariable coupling complex real-time system with a high requirement. According to the device, a problem that a conventional detection device can not obtain an ideal effect of the intelligent detection of the electric vehicle power battery SOC.

The invention relates to a method for rapidly representing sludge dewatering performance. The method comprises the following steps of: collecting 100ml of raw sludge or concentrated sludge; filtering with a filter membrane of 0.45mu m to obtain 10mL of sludge supernatant; measuring soluble organic carbon (DOC) of the supernatant; diluting the supernatant with distilled water until the DOC is less than 10mg/L; collecting a three-dimensional fluorescence spectrum (EEM) of the diluted supernatant; substituting the collected EEM spectrum into a database to analyze a parallel factor (PARAFAC) by using a DOMF1uor tool kit of Mat1ab 7.0 data processing software to determine a score of a component with excitation and transmission wavelength of (220,280)/350nm; and multiplying the score by a diluting multiple of a sample and substituting the product into a sludge dewatering performance judgment relational formula to represent the sludge dewatering performance. By the method, the sludge dewatering performance can be rapidly detected without chemical reagent, and the detection can be completed only through filtering, diluting and three-dimensional fluorescence spectrum; and the method can be applied to monitoring the dewatering performance of various types of sludge of sanitary sewage treatment factories, industrial sewage biological treatment factories and the like.

The invention relates to a fluorescence nano-material, and particularly relates to a preparation method of a fluorescence probe and applications of the fluorescence probe. The fluorescence probe has characteristics of strong fluorescence signals, high stability and good water solubility. The fluorescence probe is prepared by connecting reduced carbon quantum dots capable of emitting blue fluorescence to single-stranded DNA. The reduced carbon quantum dots are prepared from carbon quantum dots, and are prepare by subjecting the carbon quantum dots to surface modification by adopting sodium borohydride as a reductant and adopting a chemical reduction method so as to obtain the reduced carbon quantum dots capable of emitting the blue fluorescence. Detection of the target DNA is achieved by utilization of fluorescence quenching-recovery processes of the fluorescence probe.

The invention discloses an infrared spectroscopy-based rapid device special for soil nitrate nitrogen and a using method thereof. The device comprises a soil sample cell module, an excitation light source module, a detector module, a modeling system module, a result display window module and operating button modules, wherein the soil sample cell module is used for storing soil pasty samples; the excitation light source module is used for transmitting an intermediate infrared laser light source signal; an ATR (Attenuated Total Reflection) crystal module is in a diamond interface; the modeling system module is used for receiving and processing spectroscopic data information and carrying out modeling, optimizing and quantitative calculation on the content of nitrate nitrogen; the result display window module is used for carrying out digital display on the content of the nitrate nitrogen; the button modules I, II, III and IV are respectively used for sampling an ATR spectrum, saving a model algorithm, calculating an optimal model algorithm and displaying results. The device disclosed by the invention is simple in operation and less in sample demands, samples are not required to be subjected to fussy pretreatment, the detection speed is fast, no chemical reagents are used, the whole process just requires several minutes, and the device is suitable for fast sampling detection in a field and a scene.

The invention discloses an analysis method for detecting pyrazine compounds in beer. A method for combining headspace solid-phase microextraction with gas-phase chromatography/mass spectrometry analysis is adopted. The analysis method comprises the following steps of: preparation, headspace solid-phase microextraction, gas-phase chromatography/mass spectrometry analysis, and detection of components to be detected which enter a detector, wherein in the solid-phase microextraction procedure, a 75mu m polydimethylsiloxane extraction fiber head is adopted, and the extraction effect is favorable. By the method, the analysis method for detecting volatile pyrazine components in the beer is established, namely a headspace solid-phase microextraction method is combined with a gas-phase chromatography/mass spectrometry. The method has the characteristics of low sample requirement, high efficiency and the like, and is quick, simple, accurate and sensitive.

The invention relates to a fluorescence labeling tracer and a preparation method thereof. The fluorescence labeling tracer is a product which is obtained by reacting a polymer of acrylic acid-hydroxy-propyl acrylate copolymer or acrylic acid-acrylamide copolymer and an organic fluorescence derivating agent of L-tryptophan, tyrosine, sulfanilic acid or sodium sulfanilate. The fluorescence labeling tracer is high in sensitivity and selectivity. When used for detecting the concentration of a water treatment agent in an industrial water system, the fluorescence labeling tracer is quick and convenient, and high in repeatability, samples can be taken easily, the required amount of samples is small, and the fluorescence labeling tracer can be used for directly and continuously detecting the running condition of a circulating water system.

The invention belongs to a biological indicator for rapid estimation of an ionizing radiation dose, and in particular relates to an application of soluble receptor tyrosine kinase AXL (sAXL) serving as the biological indicator for the rapid estimation of the ionizing radiation dose. After radiation, the concentration of the sAXL in serum or plasma is decreased along with the increase of the radiation dose, so that a good dose-effect relationship is formed after a period of radiation and can be detected rapidly by methods which are simple and easy to implement, such as ELISA (Enzyme Linked Immunosorbent Assay) and protein chips. During the detection, the required sample quantity is small; and the sampling is carried out conveniently. Thus, the sAXL can be taken as the biological indicator for the rapid estimation of the ionizing radiation dose. In addition, the ionizing radiation dose of a human body or an animal subjected to the ionizing radiation can be estimated by utilizing the content of the sAX in serum or plasma.

The invention relates to an exosome separation and purification device and a separation and purification method thereof. The device comprises an upper micro-fluidic chip, a filter membrane and a lowermicro-fluidic chip, and the filter membrane is arranged between the upper micro-fluidic chip and the lower micro-fluidic chip. Sample liquid is injected into the upper micro-fluidic chip through oneinlet of the upper micro-fluidic chip, and after the sample liquid is filtered through the filtering membrane, an exosome is obtained from one inlet of the lower micro-fluidic chip. According to the method disclosed by the invention, impurity protein in the exosome can be removed, the whole exosome detection and analysis time of the technology is less than 1 hour, and the technology is stable. A sample obtained by the device can be directly subjected to mass spectrometry, and the main signals of a graph obtained are consistent with those of a mass spectrum detection graph obtained after exosome cyst separation and purification through an existing standard ultracentrifugation method. The method has the advantages of being low in cost, small in amount of samples needed, rapid, convenient touse, accurate, stable, capable of avoiding the defects that time and resources are consumed in the ultracentrifugation technology and the like and wide in biological and medical application prospect.

The invention belongs to a biological index for rapidly estimating the ionizing radiation dosage and particularly relates to an application of soluble CD30 (sCD30) as a biological index for rapidly estimating the ionizing radiation dosage. After radiation, the concentration of the sCD30 in blood serum or blood plasma is reduced along with the increasing of the radiation dosage; after the radiation is carried out within a certain time range, a very good dosage-effect relation exists; the rapid detection can be carried out by using a simple and feasible method through ELISA (Enzyme Linked Immunosorbent Assay), a protection chip and the like; a little amount of sample is needed by the detection and the sampling is convenient. Therefore, the sCD30 can be used as the biological index for rapidly estimating the ionizing radiation dosage; the radiation dosage of a human body and an animal after ionizing radiation is rapidly calculated by adopting the content of the sCD30 in the blood serum or the blood plasma.

The invention provides a method for detecting ketoprofen in plasma by using high-performance liquid chromatography-tandem mass spectrometry and application and relates to the technical field of chemical detection. The method for detecting ketoprofen in plasma by using high-performance liquid chromatography-tandem mass spectrometry is used for taking ketoprofen-D3 as a detection internal standard and performing quantification by using an internal standard curve method. The method is high in specificity and low in detection limit; meanwhile, relevant coefficients in the linear range of a standard curve are greater than 0.999 and show that linear relation in the linear range is good and the repeatability is high; in addition, the method also has the advantages of short analysis time and low quantity of needed samples; the sample analyzing efficiency can be greatly improved; in conclusion, the whole operation process of the method for detecting ketoprofen in plasma by using high-performance liquid chromatography-tandem mass spectrometry is simple and is easy to implement; the method is scientific, reliable and controllable, is suitable for practical application and popularization and has a broad application prospect.

The invention discloses a novel microbial sensor for detecting chromium ions. The novel microbial sensor comprises a polycarbonate (PC) detection base plate, an alloy coating conduction channel is arranged on the surface of the PC detection base plate, one end of the alloy coating conduction channel is connected with a leading wire, the other end of the alloy coating conduction channel is a detection coating, a layer of PC cover plate is covered above the detection coating, a detection pool which corresponds to the detection coating is arranged on the PC cover plate, the detection coating serves as the bottom surface of the detection pool, a chromium ion induction layer is coated on the surface of the detection coating of the detection pool, the chromium ion induction layer is composed of graphite powders, ochrobactrum anthropi freeze-dried powders and liquid paraffin according to a mass ratio of 45:45:10, and the graphite powders, the ochrobactrum anthropi freeze-dried powders and the liquid paraffin are uniformly mixed and tiled on the surface of the detection coating. The novel microbial sensor for detecting the chromium ions has the advantages that the required quantity of samples is small, the detection time is short, the sensitivity is high, the lower limit of detection is low, the novel microbial sensor is extremely easy to carry about, the real-time monitoring is convenient, most importantly the manufacturing cost is low, the mass production is achieved, and the stability and the biological selectivity are high.