32results about How to "Less sample required" patented technology

The invention discloses a detection reagent containing nano mimic enzyme. The invention further discloses a visual detection method of bio-enzyme or protein and its inhibitor on the basis of nano mimic enzyme technology. The method shows good pH dependence for the oxidization reaction of many developing agents on the basis of some nano materials capable of simulating natural oxidase, and many oxidase or proteins can release / consume H+ characteristics in the biological catalysis process, and uses the in-site change of pH of reaction system to influence the catalytic oxidization ability of the nano material, thus the detection reagent has color change at different levels, and the visual detection of bio-enzyme or protein activity is realized, and further the visual detection of corresponding inhibitor is realized. The physical signal output by the detection method can show a very good linear relationship for the bio-enzyme or protein activity within a certain concentration scale, and thereby realizing the high-sensitivity detection of a target to be tested.

The invention discloses a strawberry greenhouse temperature intelligent detection device based on an empirical mode decomposition model, which is characterized in that: the detection device includes a strawberry greenhouse environment parameter acquisition platform based on a CAN field bus and a strawberry greenhouse temperature intelligent prediction system; the present invention It not only effectively solves many problems in the traditional strawberry greenhouse environment due to unreasonable design, backward equipment, and imperfect control system, but also effectively solves the existing strawberry greenhouse environmental monitoring system. Due to the characteristics of non-linearity, large lag, and complex temperature changes of the strawberry greenhouse environment, the temperature of the strawberry greenhouse environment is monitored and predicted, which greatly affects the regulation of the strawberry greenhouse environment temperature.

The invention relates to a high performance liquid chromatography method for jointly, rapidly and accurately detecting the contents of melanin and inosinic acid in chicken of silky fowl. The method comprises the following steps of: (1) carrying out protease oscillating enzymolysis on a chicken sample for 24 hours, centrifuging to obtain liquid supernatant which serves as inosinic acid to-be-detected solution; (2) orderly washing precipitate which is subjected to enzymolysis and centrifugation with diethyl ether, absolute ethyl alcohol and ultrapure water, removing lipid and impurities, so as to obtain a melanin crude product, oxidizing the crude product into 2, 3, 5-tricarboxylic acid pyrrole under alkaline condition, extracting with diethyl ether, and drying; and (3) dissolving the dry crystal with the to-be-detected inosinic acid solution, loading and detecting the sample on a high performance liquid chromatograph, and quantifying by an external standard method. The high performance liquid chromatography method only needs little sample to simultaneously detect the contents of the melanin and inosinic acid in the chicken sample of silky fowl, moreover, the method is simple in experimental operation steps, saves detection cost, and is beneficial for acceleration of screening excellent silky fowl breed (series) or combination with both outstanding functions and flavor substances.

The invention relates to the technical field of drug analysis, and specifically discloses a method for detecting related substances in rifampicin. The method comprises: preparing a test solution and a reference solution, and detecting by liquid chromatography-mass spectrometry, wherein the liquid chromatography adopts a C18 chromatographic column, and a formic acid aqueous solution with a volume concentration of 0.8‰~1.2‰ is used as a flow Phase A, using acetonitrile as mobile phase B, gradient elution; the mass spectrometer adopts ESI ion source, positive ion detection, SIM mode. The detection method provided by this application has the advantages of simple method, stability, high precision, good reproducibility, etc., and can quickly and accurately detect the impurities N-methylpiperazine and 1-amino-4- The whole operation process is reliable and controllable, suitable for practical application and promotion, and has broad application prospects.

The invention discloses a group of markers and a method for screening high-scent tea tree resources suitable for making oolong tea. The markers are composed of indole, nerolidol and jasmonolactone. The landmarks in the present invention can be applied to scoring and rapid screening of high-aroma tea tree resources suitable for making oolong tea. The method for screening high-flavor tea tree resources suitable for making oolong tea in the present invention has the advantages of simple operation, short time consumption, small sample size and high reliability, and can be applied to screening large quantities of resource samples.