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Determination method for content of irisflorentin in animal serum

A technology for the determination of irisanthin and its determination method, which is applied in the field of drug analysis, can solve the problems that the detection method of blood drug concentration has not been reported yet, and achieve the effect of simple and easy operation of the sample processing method, improved sensitivity and short analysis time

Inactive Publication Date: 2015-08-19
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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Problems solved by technology

At present, the determination of the content of irisaxanthin is mostly in vitro, but the detection method of blood drug concentration in animals has not been reported yet.

Method used

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  • Determination method for content of irisflorentin in animal serum
  • Determination method for content of irisflorentin in animal serum
  • Determination method for content of irisflorentin in animal serum

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Embodiment 1

[0029] In order to further illustrate the essence of the present invention, the applicant has carried out the following experimental analysis to the method of the present invention.

[0030] The Shegan extract used in the present invention is prepared by Lanzhou Institute of Animal Husbandry and Veterinary Medicine, with kaempferol as the internal standard substance.

[0031] 1 Materials and methods

[0032] 1.1 Instruments 6410 type triple quadrupole mass spectrometer (Aglient, USA), 200 type high performance liquid chromatography (Aglient, USA), electrospray ionization source (ESI), high-speed centrifuge (Allegra X-15R), vortex mixer (Equl- Glascol).

[0033] 1.2 Drugs and Reagents Standard Substance of Irisaxanthin (National Institute for the Control of Pharmaceutical and Biological Products); Shegan Extract (prepared by Lanzhou Institute of Animal Husbandry and Veterinary Medicine); Kaempferol (sigma); Acetonitrile is chromatographically pure, ethyl acetate is chemically ...

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Abstract

The invention discloses a determination method for the content of irisflorentin in animal serum. The high performance liquid chromatography-mass spectrometry technology is applied to determination of the content of irisflorentin in animal serum; and kaempferol is used as internal standard for determination of the content of irisflorentin in animal serum. According to the method, sample treatment is simple and easy to operate, and a recovery rate meets requirements of analysis and detection; and as the HPLC-MS / MS technology is applied to determination of the content of irisflorentin in animal serum, detection sensitivity of detected components is improved, good resolution is realized, analysis time is short, the desired amount of a sample is small, the usage amount of a solvent is small, content determination results are accurate, and repeatability and good stability are achieved.

Description

technical field [0001] The invention relates to a method for measuring the content of irisaxanthin in animal serum by using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS), and belongs to the field of drug analysis. Background technique [0002] Shegan is the dried rhizome of Shegan, a plant of the genus Shegan in the family Iridaceae. It is a commonly used medicinal material with the effects of clearing away heat and detoxification, relieving throat and eliminating phlegm. It is mainly used clinically for sore throat, phlegm, cough and asthma. Subfield irisaxanthin is one of the important isoflavone components in Shegan, and it is also an indicative component for evaluating the quality of Shegan medicinal materials in the Pharmacopoeia. At present, the determination of irisaxanthin content is mostly in vitro, but the detection method of blood drug concentration in animals has not been reported yet. In recent years, more and more studies have sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 辛蕊华李维郑继方罗永江谢家声王贵波罗超应李锦宇
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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