Analysis method for detecting pyrazine compounds in beer

An analysis method and compound technology, which are applied in the analysis field of detecting pyrazine compounds in beer to achieve the effects of less interfering impurity peaks and less sample required.

Inactive Publication Date: 2011-06-15
TSINGTAO BREWERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This technique has never been applied to the detection of pyrazines in beer before

Method used

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  • Analysis method for detecting pyrazine compounds in beer
  • Analysis method for detecting pyrazine compounds in beer
  • Analysis method for detecting pyrazine compounds in beer

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Embodiment

[0035] The analysis method for the detection of pyrazine compounds in beer provided by this embodiment adopts the method of combining headspace solid-phase microextraction and gas chromatography / mass spectrometry, including the following steps:

[0036] The first step, preparation: exhaust the liquor to be tested, take 10ml and add it to the headspace bottle;

[0037] The second step, headspace solid-phase microextraction (HS-SPME): Edit the program so that the solid-phase microextraction head is inserted into the top of the headspace bottle, and the extraction is performed at a constant temperature; the 75μm Carboxen-PDMS extraction fiber head is used to extract the fiber When the head is used for the first time, it is activated at the gas chromatograph inlet at 270°C for 1 hour; 10mL of vented beer is added to a 20mL headspace bottle and 4g of sodium chloride is added; the adsorption time is 40min; the adsorption temperature is 50°C.

[0038] The third step, gas chromatograp...

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Abstract

The invention discloses an analysis method for detecting pyrazine compounds in beer. A method for combining headspace solid-phase microextraction with gas-phase chromatography / mass spectrometry analysis is adopted. The analysis method comprises the following steps of: preparation, headspace solid-phase microextraction, gas-phase chromatography / mass spectrometry analysis, and detection of components to be detected which enter a detector, wherein in the solid-phase microextraction procedure, a 75mu m polydimethylsiloxane extraction fiber head is adopted, and the extraction effect is favorable. By the method, the analysis method for detecting volatile pyrazine components in the beer is established, namely a headspace solid-phase microextraction method is combined with a gas-phase chromatography / mass spectrometry. The method has the characteristics of low sample requirement, high efficiency and the like, and is quick, simple, accurate and sensitive.

Description

technical field [0001] The invention belongs to a beer analysis and detection method, in particular to an analysis method for detecting pyrazine compounds in beer. Background technique [0002] Through years of research, foreign scientific researchers have found that amino acids and sugars in malting barley undergo a series of processes such as malting, saccharification, and fermentation, and Maillard reactions occur to produce compounds such as pyrazines, furans, and pyrans. The basic backbone of malt aroma, they can give beer a special malt flavor. Pyrazine compounds are nitrogen-containing six-membered heterocyclic components. Although their content in beer is not high, their special flavor characteristics and low threshold make them the key substances of beer malt aroma. In pyrazine compounds, 2-methylpyrazine, 2,5-dimethylpyrazine, 2,6-dimethylpyrazine, 2-ethylpyrazine, 2,3-dimethylpyrazine , 2,3-diethylpyrazine, and 3-ethyl-2methylpyrazine are more prominent, and the...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72
Inventor 陈华磊郝俊光闫鹏李梅张宇昕田玉红杨梅王书谦娄晓红梁刚
Owner TSINGTAO BREWERY
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