Device and method for measuring respiration of microbes

Abstract

The invention provides a device for measuring the respiration of microbes. The device is equipped with a tubular reaction cavity, wherein, the inner wall of the tubular reaction cavity is equipped with a reaction sieve which divides the reaction cavity into an upper part and a lower part; the orifice of the tubular reaction cavity is equipped with a seal plug; the seal plug is equipped with a through hole; an electrode tip hermetically passes through the through hole to extend into the upper part of the tubular reaction cavity; the tubular reaction cavity is vertically placed in a thermostatic water bath, and the thermostatic water bath is equipped with a water inlet and a water outlet which are communicated with a thermostatic water tank for circulation; the bottom surface of the tubularreaction cavity is contacted with the bottom surface of the thermostatic water bath; the top of the thermostatic water bath is equipped with a hole which is hermetically matched with the external diameter of the orifice of the tubular reaction cavity; a magnetic sheet is placed on the bottom surface of the tubular reaction cavity; the bottom surface of the thermostatic water bath is placed on a magnetic stirrer; the tubular reaction cavity is filled with a reaction solution; and a biological sample is placed on the reaction sieve, and the sieve pore is smaller than the volume of the biological sample.

Description

technical field [0001] The invention relates to the research and measurement of biological respiration, in particular to a device and method for measuring microbiological respiration for research on microorganisms and small organisms in biofilms, sediments or water bodies. Background technique [0002] Oxygen in surface water is the basic condition for sustaining organisms, such as fish and benthic animals, and is one of the most important reactants in the biogeochemical cycle. The utilization of oxygen by aquatic organisms is an important driving force for the consumption of oxygen in water and the degradation of organic matter; the aerobic respiration of organisms refers to the complete oxidation of organic matter such as sugars by the organisms under the participation of aerobic oxygen through the catalysis of enzymes Decomposition produces carbon dioxide and water while releasing a large amount of energy. Accurately measuring the rate of oxygen utilization by aquatic or...

AI Technical Summary

Problems solved by technology

However, the current determination methods are not suitable for the determination of small volume biological oxygen consumption

For example, epiphyllous biofilm or stone biofilm itself contains less microbial biomass, and a larger volume measurement system is difficult to sensitively reflect the respiration of biofilm

For another example, when measuring the respiration of water earthworms or chironomus larvae, it is also difficult for a large-volume measurement system to reflect small changes in oxygen.

Therefore, there is still a large gap in the current measurement method for the respiration of tiny organisms, and a simple and practical method is urgently needed to make up for it.

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