Application of soluble receptor tyrosine kinase AXL serving as biological indicator for rapid estimation of ionizing radiation dose

A technology of tyrosine kinase and ionizing radiation, applied in the direction of biological testing, material inspection products, etc., can solve the problems of complexity and long detection cycle, and achieve the effect of less demand, short detection time and objective results

Inactive Publication Date: 2013-10-09
NAVY MEDICINE RES INST OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the rapid development of cell and molecular biology, some progress has been made in the research on ionizing radiation biological dosimeters at home and abroad, including chromosomal aberrations, lymphocyte micronuclei, precocious condensed chromosomes, and fluorescence in situ hybridization. However, traditional biological dosimeters such as chromosomal aberration analysis methods are relatively complicated, require cell culture, and the detection cycle is long, usually requiring 2 ~3 days, with some limitations

Method used

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  • Application of soluble receptor tyrosine kinase AXL serving as biological indicator for rapid estimation of ionizing radiation dose
  • Application of soluble receptor tyrosine kinase AXL serving as biological indicator for rapid estimation of ionizing radiation dose
  • Application of soluble receptor tyrosine kinase AXL serving as biological indicator for rapid estimation of ionizing radiation dose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Implementation Example 1: Establishment of Mouse Irradiation Model and Sample Collection

[0026] Experimental animals: male C57BL / 6 mice, 7 weeks old, weighing 19±1g, purchased from Shanghai Slack Experimental Animal Co., Ltd., animal quality certificate number: 2007000534826, experimental animal production license number SCXK (Shanghai) 2007- 0005.

[0027] Serum preparation: Mice were irradiated at 60 In the cobalt source room, 80 mice were randomly divided into four groups, 20 in each group: 1 group in the control group and 4 groups in the radiation group. The mice in the radiation group received 1Gy, 3Gy, 5Gy and 10Gy total body irradiation respectively at a dose rate of 0.78Gy / min. 24h, 48h, and 72h after irradiation, blood was collected from the mice through the eyeball. The whole blood was clotted at 37°C for 1h, centrifuged at 3000rpm×15min, and the supernatant serum was separated and distributed in centrifuge tubes at 100μl / tube. Aliquoted serum was frozen ...

Embodiment 2

[0028] Implementation example two: detection of sAXL content in mouse serum

[0029] (1) Detection of sAXL content in serum of non-irradiated mice

[0030] Testing materials and methods:

[0031] Cytokine antibody chip detection. The chip name is: Custom Mouse Array chip, customized by RayBiotech. The chip detection operation steps are as follows:

[0032] ① Place the chip bag at room temperature for 30 minutes, tear off the sealing film, mark the chip, and dry it for 2 hours on a clean bench;

[0033] ②Sample preparation: 60μl serum + 60μl diluent, mix well;

[0034] ③Preparation of standard substance: take 10000pg / ml standard solution (std1), three-fold dilution 6 times (std2~7), the lowest concentration is 13.7pg / ml (std7).

[0035] ④ Preparation of negative control: Take 120 μl of diluent (CNTRL)

[0036] ⑤ Chip blocking and sample incubation: add 100 μl blocking solution to each well, block at room temperature for 0.5 h, shake gently, add 100 μl negative control...

Embodiment 3

[0059] Implementation Example 3: Estimation of Irradiation Dose in Mice

[0060] According to the concentration of serum sAXL and the irradiation time, estimate the dose of the mice. The estimation method is shown in Table 5-7.

[0061] Table 5 Estimated dose of exposure to mice within 24 hours after irradiation

[0062]

[0063] Table 6 Estimated dose of exposure to mice 24 to 48 hours after irradiation

[0064]

[0065] Table 7 Estimated dose of exposure to mice 48-72 hours after irradiation

[0066]

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Abstract

The invention belongs to a biological indicator for rapid estimation of an ionizing radiation dose, and in particular relates to an application of soluble receptor tyrosine kinase AXL (sAXL) serving as the biological indicator for the rapid estimation of the ionizing radiation dose. After radiation, the concentration of the sAXL in serum or plasma is decreased along with the increase of the radiation dose, so that a good dose-effect relationship is formed after a period of radiation and can be detected rapidly by methods which are simple and easy to implement, such as ELISA (Enzyme Linked Immunosorbent Assay) and protein chips. During the detection, the required sample quantity is small; and the sampling is carried out conveniently. Thus, the sAXL can be taken as the biological indicator for the rapid estimation of the ionizing radiation dose. In addition, the ionizing radiation dose of a human body or an animal subjected to the ionizing radiation can be estimated by utilizing the content of the sAX in serum or plasma.

Description

1 technical field [0001] The invention belongs to the field of ionizing radiation biological dose estimation. Specifically, it relates to the application of soluble receptor tyrosine kinase AXL (sAXL) as a biological indicator for rapid estimation of ionizing radiation dose. 2 background technology [0002] Countries around the world are increasingly paying attention to the application of nuclear energy. my country's nuclear power is moving from "moderate development" to "active development" and will play a very important role in the future power supply. With the rapid development of science and technology, radioactive sources and radiation technology are widely used in the fields of industry and agriculture, medicine, teaching and even military affairs. However, while nuclear technology benefits mankind, it also poses a potential threat to public safety and public health. Acute radiation sickness is the epitome of this threat. Acute radiation sickness refers to a systemi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 刘琼何颖沈先荣蒋定文侯登勇陈伟刘玉明王庆蓉钱甜甜李珂娴
Owner NAVY MEDICINE RES INST OF PLA
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