Application of headspace gas chromatography in determination of low-concentration trimethylamine in chicken

A technology of trimethylamine and chromatography, applied in the field of trimethylamine detection, can solve the problems of inability to effectively detect low-concentration trimethylamine content in chicken, and achieve the effects of improving sensitivity and repeatability, improving peak shape, and high accuracy

Pending Publication Date: 2021-02-19
ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The published technology cannot effectively detect the content of low-concentration trimethylamine in chicken, s

Method used

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  • Application of headspace gas chromatography in determination of low-concentration trimethylamine in chicken
  • Application of headspace gas chromatography in determination of low-concentration trimethylamine in chicken
  • Application of headspace gas chromatography in determination of low-concentration trimethylamine in chicken

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Embodiment 1

[0042] A method for the determination of low-concentration trimethylamine in chicken meat by headspace gas chromatography:

[0043] 1) Reagent preparation:

[0044] 15mol / L lye: Accurately weigh the alkaline substance and dissolve it in water to prepare a certain concentration of lye;

[0045] 5% trichloroacetic acid solution: Weigh 25g of trichloroacetic acid and dissolve it in water, and set the volume to 500mL.

[0046] 2) Sample pretreatment and acquisition of sample solution: Accurately weigh 10g of chicken tissue that has been crushed, extract it with 20mL, 15mL, and 10mL of 5% trichloroacetic acid solution, fully precipitate the protein in the tissue, and filter to get the supernatant ,Volume;

[0047] 3) Preparation of standard stock solution: Accurately weigh 0.1622g of trimethylamine hydrochloride standard product, dissolve in 5% trichloroacetic acid solution and dilute to 100mL, the concentration is 1000μg / mL

[0048] 4) Preparation of standard working solution: ...

Embodiment 2

[0063] A method for the determination of low-concentration trimethylamine in chicken meat by headspace gas chromatography:

[0064] 1. Reagent preparation:

[0065] 1) 15mol / L lye: Accurately weigh 60g of sodium hydroxide, dissolve it in water, and set the volume to 100mL;

[0066] 2) 5% trichloroacetic acid solution: weigh 25g of trichloroacetic acid, dissolve it in water, and set the volume to 500mL;

[0067] 3) Preparation of trimethylamine standard stock solution: Accurately weigh 0.1622g of trimethylamine hydrochloride standard product, dissolve it in 5% trichloroacetic acid solution and set the volume to 100mL, the concentration is 1000μg / mL trimethylamine standard stock solution, 4 Store at ℃;

[0068] 4) Preparation of trimethylamine standard working solution: Take a certain volume of trimethylamine standard stock solution and dilute it step by step with 5% trichloroacetic acid solution to obtain concentrations of 40.0 μg / mL, 20.0 μg / mL, 10.0 μg / mL, 5.0 μg / mL, respec...

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Abstract

The invention belongs to the technical field of trimethylamine detection, and particularly relates to application of headspace gas chromatography in determination of low-concentration trimethylamine in chicken. The headspace gas chromatography can be used for detecting the content of low-concentration trimethylamine in chicken, and is applied to detection of fresh meat, refrigerated meat and chicken samples stored for a long time. The detection method of the headspace gas chromatography comprises the following steps of preparing alkali liquor, a trichloroacetic acid solution, a trimethylaminestandard stock solution and a trimethylamine standard working solution, drawing a trimethylamine standard curve and detecting a sample extracting solution. By optimizing parameters such as alkali liquor concentration, headspace balance temperature and carrier gas flow in headspace gas chromatography, the peak shape of a detected substance is effectively improved, and the detection sensitivity andrepeatability are improved; the method is suitable for determining the content of trimethylamine in chicken, particularly has an accurate determination effect on the low-concentration content, is short in balance time and high in accuracy, and is suitable for detecting various samples such as raw fresh meat, refrigerated meat and chicken stored for a long time.

Description

technical field [0001] The invention belongs to the technical field of trimethylamine detection, and in particular relates to an application of headspace chromatography in the determination of low-concentration trimethylamine in chicken. Background technique [0002] China is the country with the largest amount of poultry raising in the world, and its chicken output ranks second in the world. In the past, the consumption of fresh and live broilers was the mainstay. However, due to the frequent occurrence of "H7N9" and other incidents in recent years, which have caused serious damage to the chicken industry, coupled with the impact of government decisions, my country's broiler industry is facing tremendous changes. In order to promote the health and sustainability of the broiler industry The development and quality and safety of poultry products, centralized slaughtering and fresh marketing have become the only way for the development of the poultry industry. [0003] Meat an...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8634
Inventor 李莹瞿浩罗成龙计坚王艳王劼舒鼎铭
Owner ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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