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Method for determining glucose, fructose and saccharose in tobacco essence perfume

A technology for tobacco flavor and glucose, applied in the field of tobacco, can solve the problems of low detection sensitivity, large baseline fluctuation, large chromatographic column, etc., and achieve the effects of good repeatability, low detection limit, repeatability and reproducibility

Inactive Publication Date: 2012-06-27
CHINA TOBACCO GUANGDONG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Use volatile solvents and chromatographic columns with large column loss, the baseline fluctuation will be relatively large, and the detection sensitivity will be low

Method used

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  • Method for determining glucose, fructose and saccharose in tobacco essence perfume
  • Method for determining glucose, fructose and saccharose in tobacco essence perfume
  • Method for determining glucose, fructose and saccharose in tobacco essence perfume

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Reagents and materials

[0026] Sucrose and D(-)-fructose, D-(+)-anhydroglucose (≥99.5%, HPLC), Sigma-Aldrich;

[0027] Acetonitrile (≥99.9%, GC), MERCK company;

[0028] Sodium hydroxide, Pure Chemical Analysis Co., Ltd., Belgium;

[0029] Milli-Q grade water, prepared by Milliore Element A10, unless otherwise specified, the water used in the experiment is Milli-Q grade water;

[0030] Tobacco essence, tobacco flavor.

[0031] instrument

[0032] 1200HPLC from Agilent, USA, equipped with G1378B degasser, G1311A quaternary pump, G1329A autosampler, G1316A column thermostat and G1362A differential refractive index detector;

[0033] American Millipore Company Milli-Q Element A10 pure water machine.

[0034] experimental method

[0035] 1. Chromatographic conditions Metacarb Pb PLUS chromatographic column (300mm × 7.8mm) of Varian Company, Metacarb 87P pre-column core. Injection volume: 20μL; flow rate: 0.4mL / min; pump stop time: 63min; column oven: 80°C; temperatu...

Embodiment 2

[0077] Embodiment 2, linearity, correlation coefficient, detection limit of the method of the present invention

[0078] Quantification by external standard method. With the concentration (μg / mL) as the abscissa x and the peak area as the ordinate y, a linear regression was performed to obtain the linear regression equation and correlation coefficient. The results show that the linearity of the method of the present invention is good, and the correlation coefficients are all greater than 0.9999 (see Table 6). With a signal-to-noise ratio (S / N) of 3, the detection limit was determined by serially diluting low-concentration standard solutions. The detection limit of the method is lower than 1.8 μg / mL, and the sensitivity is good.

[0079] Table 6 Linear equation, correlation coefficient, detection limit of standard curve

[0080]

Embodiment 3

[0081] Embodiment 3, method repeatability test of the present invention

[0082] According to the processing method described in 3 in Example 1, sample A was carried out 8 parallel measurements in 1 day to obtain the intraday precision of the method; the sample was measured 6 times in 8 days to obtain the interday precision of the method. The intra-day and inter-day precision results are expressed as RSD%, and the results are shown in Table 7 and Table 8, respectively. The intraday precision of fructose, glucose and sucrose was less than 1.2%, and the interday precision was less than 3.0%. The repeatability of the method was good.

[0083] The intraday precision test results of the method in Table 7

[0084]

[0085] The day-to-day precision test results of the method in Table 8

[0086]

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Abstract

The invention discloses a method for determining glucose, fructose and saccharose in tobacco essence perfume through a high performance liquid chromatography / differential refraction detector. The method comprises the following steps: dissolving a sample through 0.01 mol / L of caustic soda solution to be constant volume; adopting a cation exchange chromatography column, which takes highly cross-linked lead type sulfonated styrene-divinylbenzene (SDVB) resin as a filler, as a separation column; using pure water as a moving phase to carry out isogradient elution; detecting through the differential refraction detector; using an external standard method for quantification; and computing contents of the glucose, the fructose and the saccharose. The method has favorable linearity with correlation coefficient being greater than 0.999, recovery of 97.1 percent-102.4 percent, within-day precision being less than 1.2 percent, day to day precision being less than 3.0 percent, detection limit being lower than 1.8mug, and good sensitivity, and is suitable for analyzing and determining the glucose, the fructose and the saccharose for various tobacco essence perfume samples.

Description

technical field [0001] The invention relates to the field of tobacco, in particular to a method for measuring glucose, fructose and sucrose in flavors and fragrances for tobacco. Background technique [0002] Flavors and fragrances are widely used in cigarette production and play an important role in regulating and improving the taste of cigarettes. A large number of flavors and fragrances contain sugar substances, among which water-soluble glucose, fructose and sucrose are more common. These sugars can react with various components in tobacco during the production and smoking of cigarettes, such as the Maillard reaction with amino acids in tobacco, which can affect the production of the characteristic aroma of cigarettes and the formation of sweet and moist taste. , plays an extremely important role. [0003] At present, the quality analysis of flavors and fragrances by gas chromatography / mass spectrometry can only obtain the volatile and semi-volatile fingerprints, but ca...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 黄翼飞卓浩廉孔浩辉
Owner CHINA TOBACCO GUANGDONG IND
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