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Method and system for detecting staphylococcus aureus in chicken

A staphylococcus, detection method technology, applied in the direction of color/spectral characteristic measurement, test sample preparation, etc., can solve the problems of low efficiency, large destructive, complicated detection steps of Staphylococcus aureus

Pending Publication Date: 2022-03-29
ZHEJIANG UNIV
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Problems solved by technology

[0004] Aiming at the problems of complex detection steps, low efficiency and high destructiveness of Staphylococcus aureus in poultry meat products, the present invention proposes a detection method and system for Staphylococcus aureus in chicken, which can simply, quickly and non-destructively detect Staphylococcus aureus to meet the needs of quality inspection in the process of chicken product processing and sales

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  • Method and system for detecting staphylococcus aureus in chicken
  • Method and system for detecting staphylococcus aureus in chicken
  • Method and system for detecting staphylococcus aureus in chicken

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[0078] 1. Use LB medium to cultivate Staphylococcus aureus, select the typical colony of Staphylococcus aureus after proliferation, mix with sterile distilled water, and obtain Staphylococcus aureus bacterial liquid, the concentration is 3logCFU / ml, 5logCFU / ml and 6logCFU / ml respectively ml; After the chicken breast was sliced, some samples were sterilized by ultraviolet light to obtain healthy chicken samples; some samples were soaked in Staphylococcus aureus bacteria solution to obtain infected chicken samples.

[0079] 2. Use a hyperspectral imager to obtain the original hyperspectral reflectance images in the 379-1023nm band of Staphylococcus aureus, healthy chicken samples, and infected chicken samples in LB medium by using a vertical linear scanning method.

[0080] 3. Perform black-and-white correction on the acquired hyperspectral image, and use ENVI software to manually select and extract the hyperspectral image of the area of ​​interest in the chicken sample to remove...

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Abstract

The invention discloses a method and a system for detecting staphylococcus aureus in chicken. The method comprises the following steps: acquiring a sample hyperspectral image; selecting a spectral image of a characteristic wavelength based on the sample hyperspectral image, setting a gray threshold, and performing segmentation processing on the selected spectral image to obtain a chicken sample to-be-detected area and a staphylococcus aureus to-be-detected area; extracting hyperspectral data; after the chicken sample hyperspectral data and the staphylococcus aureus hyperspectral data are mixed, characteristic wavelengths are extracted; selecting chicken sample hyperspectral data corresponding to the extracted characteristic wavelength to train a support vector machine model to obtain a staphylococcus aureus detection model; and detecting the staphylococcus aureus in the chicken by using the staphylococcus aureus detection model. The method can be used for simply, quickly and nondestructively detecting the staphylococcus aureus in the chicken product so as to meet the requirements of quality detection in the processing and selling processes of the chicken product.

Description

technical field [0001] The invention relates to the technical field of non-destructive rapid detection of food quality and safety, in particular to a detection method and system for Staphylococcus aureus in chicken. Background technique [0002] Poultry meat products are easily contaminated by various food-borne pathogens during processing, which reduces the quality of poultry meat products and causes food safety problems. Staphylococcus aureus is a common pathogen. Eating meat products contaminated by Staphylococcus aureus can easily lead to vomiting, diarrhea and other symptoms. For the detection of Staphylococcus aureus in food, biochemical identification is usually used. The national standard "Food Microbiological Examination-Staphylococcus aureus Examination" (GB4789.10-2016) provides a detection method under laboratory conditions, but the method is complicated to operate and requires relatively high professional skills and laboratory environmental conditions. High, d...

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Application Information

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IPC IPC(8): G01N1/28G01N21/27
CPCG01N1/28G01N21/27G06T2207/30128G06T7/0004G01N33/12G01N21/31G01N21/94G01N2201/129G16H30/40G16H50/30G16H50/20G06V10/143G06V20/695G06V20/698G06T7/136G06T7/11G16B40/00G01N21/314G06T2207/20081G06T5/70
Inventor 仇瑞承何勇
Owner ZHEJIANG UNIV
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