Method for detecting freshness of chicken by electrochemical method

A freshness, electrochemical technology, applied in the direction of electrochemical variables of materials, testing food, material inspection products, etc., can solve the problems of high experimental operation requirements and insufficient accuracy, and achieve low requirements for inspectors, simple steps, and detection costs. low cost effect

Active Publication Date: 2019-06-18
山东大鼓国际供应链管理有限公司
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  • Method for detecting freshness of chicken by electrochemical method
  • Method for detecting freshness of chicken by electrochemical method

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Embodiment 1

[0033] The present embodiment is chicken breast meat, chicken wing, chicken leg freshness detection, comprises the following steps:

[0034] 1. Acquisition of electrochemical scanning spectra of different parts of chicken with different freshness

[0035] (1) Clean the chicken breast, chicken wings, and chicken leg samples stored at 5°C for 0-10 days in deionized water, and dry the surface of the chicken breast with absorbent paper;

[0036] (2) Take 1 g of the pretreated chicken breast and grind it into minced meat, add 2 mL of ethanol as a solvent to mix and sonicate for 1 min;

[0037] (3) Use 200nm pore size polytetrafluoroethylene filter paper to filter the fully mixed sample, take 1 μL of the filtrate and apply it to the surface of the glassy carbon electrode and let it dry naturally, insert the modified electrode into 10mL of 0.1mol / L phosphate buffer solution In this method, linear voltammetry scanning detection is carried out; the scanning detection conditions are as...

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Abstract

The invention discloses a method for detecting the freshness of the chicken by an electrochemical method. The method comprises the following steps: (1), washing chicken samples with different freshness values in deionized water, sucking the moisture, smashing the chicken samples, weighing the samples, adding a solvent, carrying out ultrasonic extraction, and carrying out filtering to obtain filterliquor; (2), dripping and coating the filter liquor on the surface of a glassy carbon electrode, carrying out drying, immersing the electrode into a buffer solution, forming a three-electrode systemby the immersed electrode, a platinum electrode and a silver/silver chloride electrode, and carrying out electrochemical response spectrum acquisition on the filter liquor by using a linear voltammetry scanning method; (3), carrying out electrochemical response spectrum acquisition on chicken samples with unknown freshness values according to the process in the step (2); and (4), carrying out dataprocessing on collected electrochemical response spectra, extracting feature values, comparing differences of feature values of the samples with the unknown freshness values with samples with the known freshness values, and determining freshness values of the chicken samples. The detecting method has advantages of simple operation, sensitive reaction, low instrument cost, high speed and high accuracy and the like.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to a method for detecting the freshness of chicken meat by electrochemical methods. Background technique [0002] Chicken is a food that people eat daily. It contains a lot of protein and various trace elements, and is widely favored by consumers. However, chicken is extremely susceptible to bacterial infection during the process from slaughter to market. Especially fresh cut meat, because the surface is moist, is a natural petri dish for the survival and reproduction of microorganisms. Microorganisms accumulate on the surface of meat for a long time, which will cause meat to spoil. Deteriorated meat not only tastes poor, but also causes serious harm to the human body. Therefore, the detection of chicken freshness has important practical value. [0003] At present, traditional bacteriological testing methods and serological methods are still commonly used in th...

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Application Information

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IPC IPC(8): G01N27/48G01N33/12
Inventor 傅力徐玉婷
Owner 山东大鼓国际供应链管理有限公司
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