145 results about "Methyl t-butyl ether" patented technology

The invention provides a preparation method of tiall peptide, and belongs to the technical field of polypeptide synthesis, according to the preparation method, a small amount of lysis solution is adopted for lysis, a small amount of poor solution composed of methyl tert-butyl ether and n-heptane with the volume ratio of 1-2: 1 is used for crude peptide precipitation, precipitated particles are uniform, large and not prone to caking, the crude peptide impurity level is lower, and the purity of the crude peptide is higher. The method has the advantages that the method is simple in process and easy in subsequent purification step, the amount of generated waste liquid is small, the activating agent 7-(1H-pyrrolo [2, 3-b] pyridine-1-yl)-1H-benzo [d] [1, 2, 3] triazole-1-alcohol is used for activating amino acid, the coupling reaction degree is high, and the target peptide yield is high. The preparation method of the tilpotide has the advantages of being simple, economical, capable of achieving mass production, low in impurity content, high in purification yield, high in yield and the like.

The invention provides a method for simultaneously detecting perfluorinated compounds in mouse tissues and organs, and belongs to the technical field of analysis and detection. The method provided by the invention comprises the following steps: mixing a mouse visceral organ tissue sample, an alkaline buffer solution and an internal standard solution, and homogenizing to obtain homogenate; mixing the homogenate with tetrabutylammonium hydrogen sulfate and methyl tert-butyl ether, and carrying out liquid-liquid extraction to obtain extract liquor; and sequentially drying and redissolving the extract liquor, and carrying out ultra-high performance liquid chromatography-tandem mass spectrometry analysis to obtain a result of the perfluorinated compound in the mouse tissue visceral organs. The result of the embodiment shows that the adding standard recovery rate of 14 target objects in the method provided by the invention is 64.9%-120.3%, the RSD of 6 repeated tests is 1.0%-14.0%, the matrix effect of the target objects is 83.7%-134.0%, and the matrix effect is relatively low; and the detection result has excellent accuracy and reproducibility.

The invention relates to the technical field of high-molecular polyurethane foaming materials, and provides a foaming material for a polyurethane material and a preparation method and application thereof.The foaming material is prepared from, by weight, 60-80 parts of methyl tertiary butyl ether, 10-20 parts of a foaming agent, 0.5-2 parts of a foaming stabilizer, 2-5 parts of a catalyst, 4-10 parts of a composite flame retardant and 0.5-3 parts of glycerin; and 1-5 parts of water. The foaming material for the polyurethane material provided by the invention has the characteristics of good foaming performance and flame retardance, and can be widely applied to the field of building thermal insulation.

The invention relates to the technical field of adhesives, and discloses an imidazolium ionic liquid and a preparation method and application thereof, the preparation method comprises the following steps: reacting omega-bromoalcohol with 1-(methoxymethyl) imidazole in acetonitrile, and washing with methyl tert-butyl ether to obtain a target ionic liquid precursor; and dissolving the product in acetonitrile, adding 4-trifluoromethyl phenyl isocyanate, and washing with methyl tert-butyl ether after the reaction is finished, thereby obtaining the target imidazolium ionic liquid. The imidazolium ionic liquid disclosed by the invention is applied to a PVB (Polyvinyl Butyral) adhesive, and the PVB adhesive shows excellent adhesion strength in a macroscopic adhesion performance test on the basis of multiple interactions, and particularly has excellent adhesion strength on aluminum. The obtained adhesive can be universally adapted to various substrates such as metal substrates and macromolecule substrates, and finally high-strength and high-durability intelligent adhesion performance is achieved.

The present application relates to the field of environmental protection additives, specifically disclosing a natural gas low-temperature combustion-supporting environmental protection additive and its preparation method. The natural gas low-temperature combustion-supporting environmental protection additive comprises the following substances in parts by weight: 10 to 50 parts of dimethyl carbonate, 0 to 10 parts of methyl tert-butyl ether, 10 to 20 parts of methanol, and 15 to 20 parts of dispersed catalytic particles; the dispersed catalytic particles are carbon aerogels adsorbed with nano-Pt particles. The natural gas low-temperature combustion-supporting environmental protection additive of the present application uses dimethyl carbonate, methyl tert-butyl ether, and methanol as liquid matrices, and a carbon aerogel material adsorbed with the precious metal Pt as a catalytic material. The environmental protection additive material is effectively coated and loaded in the boiling gas phase. A uniform bonding form is formed between the gas-phase environmental protection additive and the natural gas, so that the methane in the natural gas opens the gas-hydrogen-oxygen bond under the action of the environmental protection additive at 80 to 100°C, effectively catalyzing the combustion of natural gas in an oxygen-rich state, further improving the activity of natural gas in a low-temperature environment.

The invention belongs to the technical field of cables, and particularly relates to a weather-proof new energy automobile charging cable which is composed of a cable core, a shielding layer wrapping the cable core and a sheath layer wrapping the shielding layer. Each cable core is composed of a conductor and an insulating layer. The invention discloses a preparation method of a traditional Chinese medicine. A sheath layer of the weather-resistant new energy automobile charging cable is prepared from 70 to 90 parts of thermoplastic elastomer (TPE), 20 to 30 parts of lignin, 25 to 30 parts of propylene oxide, 20 to 25 parts of furfuryl alcohol, 2 to 5 parts of 4-chloromethyl styrene, 1.5 to 3 parts of tetrabutyl ammonium hydroxide, 5 to 6 parts of methylbenzene, 10 to 25 parts of methyl tert-butyl ether, 45 to 7 parts of anhydrous MgSO4, 0.5 to 2.5 parts of initiator, 2 to 3 parts of cross-linking agent, 5 to 8 parts of metal salt additive and 1 to 3 parts of lubricant. 0.3 to 0.6 part of antioxidant and 3 to 4 parts of color master batch.

The present invention relates to the technical field of clean fuels, and in particular to an ether-based clean fuel for vehicles and a preparation method thereof, comprising the following raw materials in parts by weight: 35-45 parts of methyl tert-butyl ether, 15-20 parts of isobutanol, 10-15 parts of methanol, 4-7 parts of flaky graphene agents based on doping cerium / yttrium, 2-4 parts of dimethyl ether, 1-3 parts of ferrocene, and 3-6 parts of regulating modifiers. The clean fuel of the present invention adopts methyl tert-butyl ether to match isobutanol, methanol, dimethyl ether and ferrocene raw materials, and the flaky graphene agent based on doping cerium / yttrium and the regulating modifier are added to coordinate and synergize, so that the combustion performance of the product is excellent, and the corrosion performance and water resistance of the product are remarkable, the product performance balance and coordination are improved, and the product has a remarkable product performance stability effect in a low temperature environment.

The invention discloses a highly flame-retardant and environmentally friendly modified PVC material without antimony trioxide and a preparation process thereof, and belongs to the technical field of modified PVC material preparation. The highly flame-retardant and environmentally friendly modified PVC material without antimony trioxide is composed of the following components in parts by weight: 40-60 parts of PVC, 2-8 parts of PVC modifier, 10-15 parts of microcapsule flame retardant, 2-8 parts of tetrahydrofuran, 0.5-2.1 parts of antioxidant, 2-4 parts of coupling agent, wherein the PVC modifier is prepared from diethylene glycol monovinyl ether, propylene acetate, azobisisobutyronitrile, ethyl acetate and methyl tert-butyl ether, and the microcapsule flame retardant is prepared from sodium hydroxide, choline chloride, phytic acid, corn starch, polyethylene glycol, toluene diisocyanate and pentaerythritol. PVC is modified using a PVC modifier, and a microcapsule flame retardant is added at the same time, and the prepared environmentally friendly modified PVC material has excellent flame retardant effect, rheology and toughness.

The invention discloses a preparation method of propargyl alcohol ether sodium propanesulfonate, which comprises the following steps: mixing propargyl alcohol, solid sodium hydroxide and methyl tert-butyl ether in a reaction container connected with a water segregator, carrying out primary heating reaction under a vacuum condition to obtain a sodium propargyl alcohol crude product, continuously dropwise adding 1, 3-propanediol into the reaction container under a normal pressure condition, and carrying out secondary heating reaction under a vacuum condition to obtain a sodium propargyl alcohol ether sodium propanesulfonate product. Adding 1, 3-propane sultone, and carrying out secondary heating reaction to obtain a propargyl alcohol ether sodium propanesulfonate crude product; and adding water to dissolve the propargyl alcohol ether sodium propanesulfonate crude product, layering, dehydrating the obtained water phase, and recrystallizing to obtain the propargyl alcohol ether sodium propanesulfonate. The methyl tert-butyl ether is utilized to carry water in vacuum, the reaction is promoted to proceed forward, the conversion rate of the sodium propargyl alcohol is improved, the reaction temperature is reduced, the yield and quality of the product sodium propargyl alcohol ether propane sulfonate are improved, amplified production is facilitated, the production cost is reduced, and the risk of the reaction process is reduced.

The utility model provides a methyl tert-butyl ether production device with switchable reactors, which is characterized in that a plurality of reactors are arranged, and a communicating pipeline and a switching valve are arranged between a feeding system and each reactor, so that raw materials C4 and methanol can selectively enter one of the reactors through valve switching operation; and the priority of the three reactors can be adjusted at will according to the activity condition of the catalyst in the production process, and any reactor can be independently switched and used without influencing the normal operation of the device.

The invention relates to a device and a method for preparing isobutene and methanol by cracking methyl tert-butyl ether. The device comprises a methyl tert-butyl ether vaporization refining tower, a reactor, a cooling tower, an absorption tower, a methanol extraction tower, an isobutene light component removal tower and an isobutene heavy component removal tower which are sequentially connected along a material conveying direction, a top discharge hole of the cooling tower is connected with a lower gas-phase material inlet of the absorption tower; an absorbent outlet at the bottom of the absorption tower is circularly connected with a cooling absorption liquid inlet at the top of the cooling tower; the bottom extract liquor outlet of the methanol extraction tower is circularly connected with the top absorbent inlet of the absorption tower; an isobutylene discharge hole is formed in the top of the isobutylene heavy component removal tower; the device further comprises a methanol recovery tower and a methanol refining tower which are connected in sequence along the material conveying direction; a bottom cooling absorption liquid outlet of the cooling tower is connected with the methanol recovery tower; a methanol discharge hole is formed in the bottom of the methanol refining tower. The device provided by the invention not only can relax the limitation of the purity of methyl tert-butyl ether in raw materials, but also can prepare high-purity isobutene and high-purity methanol at the same time.

The invention belongs to the field of organic synthesis, and particularly relates to a synthesis method of isoindolone. An N-methylbenzylamine compound as shown in a formula 1 and a 2-fluorobenzoic acid methyl ester compound as shown in a formula 2 are mixed with methyl tert-butyl ether in the presence of bis (trimethylsilyl) lithium amide and cesium fluoride, and the isoindolone as shown in a formula 3 is synthesized through reaction. The isoindolone derivative is synthesized through a one-pot method, and the method has the prominent advantages that raw materials are simple and easy to obtain, operation is easy and convenient, economy and environment friendliness are achieved, and the substrate applicability is wide.

The application discloses a method for determining 15 new psychoactive substances in saliva by solid phase supported liquid-liquid extraction (SLE) combined with ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS / MS), which specifically uses a mixed solution of methyl tert-butyl ether and n-hexane as an elution solvent, adopts solid phase supported liquid-liquid extraction to pretreat saliva samples, and then adopts UPLC-MS / MS to detect the collected eluent, so as to realize qualitative and quantitative determination of the new psychoactive substances in saliva. The method has the advantages of rapidness, accuracy, high sensitivity and simple operation, the detection limit of the method is 0.2-0.5 ng / mL, can meet the domestic and foreign limit requirements, and provides technical support for monitoring and investigation of the new psychoactive substances in saliva.

The invention belongs to the technical field of tert-butylamine preparation, and particularly relates to a preparation process for synthesizing tert-butylamine by adopting methyl tert-butyl ether. The preparation process comprises the following steps: preparing calcium alginate microspheres; preparing an SAPO precursor solution; performing composite crystallization; preparing an SAPO-11 carrier; preparing an ammonium type SAPO-11 carrier; preparing a metal supported catalyst; preparing an activated catalyst; methyl tert-butyl ether and liquid ammonia are used as raw materials, and tert-butylamine is prepared under the catalysis of an activated catalyst. According to the preparation process for synthesizing the tert-butylamine by adopting the methyl tert-butyl ether, the process of catalytically synthesizing the tert-butylamine by taking the methyl tert-butyl ether and the ammonia as raw materials follows an acid catalytic cracking nucleophilic addition coupling mechanism, and the prepared hierarchical pore CuZn / HSAPO11 catalyst remarkably reduces the reaction activation energy through the synergistic effect of B acid, L acid and metal active sites; high-activity and high-selectivity catalytic conversion at low temperature is realized, and high-purity tert-butylamine is prepared.

The invention discloses a post-treatment method of a polypeptide lysate. The method belongs to the field of polypeptide drug preparation. The method comprises the following steps: firstly, concentrating a polypeptide lysate, and then spraying pre-cooled isopropyl ether or methyl tert-butyl ether into the concentrated solution until a large amount of samples are separated out; and carrying out solid-liquid separation by using a filter bag type centrifuge, and carrying out on-line leaching by using isopropyl ether or methyl tert-butyl ether. The solid obtained through the method is large in particle size, solid-liquid separation is facilitated, the operation time is greatly shortened, and the method is extremely beneficial to large-scale industrial production. In addition, an on-line leaching mode is adopted, so that contact with people is avoided, and green industrial production is facilitated.

The invention provides a furfuryl alcohol preparation method, and relates to the technical field of furfuryl alcohol preparation, magnesium chips are placed in methyl tert-butyl ether or a mixed solution of methyl tert-butyl ether and tetrahydrofuran, nitrogen replacement is carried out, chloroethane / chloromethane gas is introduced after nitrogen replacement, a Grignard reagent is generated through a reaction, and a solution containing the Grignard reagent is obtained; the method comprises the following steps: dropwise adding furfural into a solution containing a Grignard reagent to carry out an addition reaction, dropwise adding soft water to carry out a hydrolysis reaction when the temperature does not change, and carrying out suction filtration when the temperature does not change to obtain a mother solution; carrying out reduced pressure distillation on the obtained mother liquor to obtain furfuryl alcohol; in the Grignard reagent preparation process, methyl tert-butyl ether is used as a solvent; the solvent containing methyl tert-butyl ether reduces the initiation temperature of the Grignard reagent, avoids the coupling reaction of the generated Grignard reagent, improves the yield and purity of the Grignard reagent, and further improves the yield and purity of the prepared furfuryl alcohol.

The utility model relates to the technical field of methyl tert-butyl ether, and discloses a methyl tert-butyl ether collecting device which comprises an inert collecting barrel, a heat-conducting inert plate, a protective rubber pad, a connecting groove, a collecting position, a protective cover, a sealing cushion layer, a supporting base, a heat dissipation plate and a fixed non-slip mat. The heat-conducting inert plate is fixedly connected to the lower end of the inert collecting barrel, the protective rubber pad is fixedly connected to the upper end of the inert collecting barrel, the connecting groove is fixedly connected to the upper end of the protective rubber pad, and the collecting part is fixedly connected to the upper end of the inert collecting barrel. According to the collecting device for methyl tert-butyl ether, by improving the collecting device, the collecting mode of the collecting device is improved, in the actual using process of the collecting device, methyl tert-butyl ether can be effectively collected and stored for a long time according to needs, volatilization of the methyl tert-butyl ether is avoided, and the collecting device is environmentally friendly. And the use safety of the methyl tert-butyl ether collecting device is improved.

The invention discloses a method for preparing 2-aminomethyl-3-chloro-5-trifluoromethylpyridine hydrochloride, relates to the technical field of chemical raw material preparation, and aims to solve the problem that an existing preparation method needs to provide a high-pressure environment and is relatively dangerous. The method sequentially comprises the following steps: 1) taking 3-chloro-2-methyl-5-(trifluoromethyl) pyridine as a raw material, and adding a solvent, azodiisobutyronitrile and N-bromo-succinimide into the 3-chloro-2-methyl-5-(trifluoromethyl) pyridine; 2) adding an alkaline substance into the 2-(bromomethyl)-3-chloro-5-(trifluoromethyl) pyridine solution, reacting under a heating condition, and after the raw materials are completely reacted, adding water for extraction and drying; and 3) adding the 1-((3-chloro-5-(trifluoromethyl) pyridine-2-yl) methyl) pyrrolidine-2, 5-diketone into 31% hydrochloric acid and acetic acid, reacting under a heating condition, and after the raw materials are completely reacted, extracting by methyl tert-butyl ether, decolorizing a water layer by using activated carbon, filtering and concentrating under reduced pressure to obtain the 2-aminomethyl-3-chloro-5-trifluoromethylpyridine hydrochloride.

The utility model relates to the field of isobutylene production, and in particular to a high-efficiency reaction device for producing isobutylene. The high-efficiency reaction device for producing isobutylene comprises a reactor, a bearing seat is provided at the bottom end of the reactor, a plurality of connection blocks are fixedly installed at equal intervals on the outer ring of the bearing seat, a support column is fixedly installed at one end of the connection block away from the bearing seat, a connection pipe is symmetrically fixedly installed on one side of the reactor, a connection seat is fixedly installed on the top of the reactor, a temperature control mechanism is provided on one side of the reactor, and the temperature control mechanism is cooperatively connected with the reactor. The high-efficiency reaction device for producing isobutylene provided by the utility model can keep a suitable safety temperature of the reactor through the temperature control mechanism provided on the equipment, shorten the heating time when methyl tert-butyl ether enters the reactor, accelerate the thermal cracking rate of methyl tert-butyl ether, and thus obtain isobutylene faster.

The invention discloses bio-based polyamide with low water absorption and a preparation method thereof, and belongs to the technical field of polyamide preparation. The bio-based polyamide with the low water absorption rate is prepared from the following components in parts by weight: 30 to 50 parts of pentamethylene diamine, 15 to 30 parts of sebacic acid, 5 to 12 parts of citric acid, 5 to 10 parts of hexamethylenediamine, 2 to 5 parts of a hydrophobic auxiliary agent and 1 to 5 parts of a modified high-temperature-resistant additive, the hydrophobic additive is prepared from acetoxybenzoic acid, nonadecanoic acid, m-dimethoxybenzene, trifluoroacetic anhydride and methyl tert-butyl ether as raw materials, and the modified high-temperature-resistant additive is prepared from o-phenylenediamine, chloroacetic acid, vanillin, benzoyl bromide and thiourea as raw materials. The bio-based polyamide prepared from the substances has low water absorption and excellent thermal stability.

The invention provides a preparation method of lacosamide, which comprises the following steps: carrying out methylation reaction on a lacosamide intermediate shown as I in the presence of alkali and dimethyl carbonate to obtain lacosamide and # imgabs0 #. Dimethyl carbonate is used as a methylation reagent, so that the use of cancerogen or high toxicity is avoided, and the preparation method is environment-friendly, and is suitable for industrial production. And the reaction system can better inhibit the generation of deacetylation impurities of lacosamide, the impurities can be removed only by pulping with methyl tert-butyl ether in post-treatment, the dosage of a purification solvent is reduced, the yield is high, the chiral purity is high, and the method is suitable for industrial large-scale production.

The invention relates to a preparation method of a crosslinked benzimidazole and imine connected polymer membrane for methanol / organic matter separation. Cross-linking the benzimidazole and imine connected polymer base membrane prepared on the support in a solution containing a cross-linking agent; the crosslinking time is 0.1 to 72 hours; the crosslinking temperature is 40-80 DEG C; putting the polymer film obtained after crosslinking treatment into a drying oven at 80-300 DEG C, carrying out heat treatment for 1-72 hours, and then naturally cooling to room temperature, so as to obtain a crosslinked benzimidazole and imine connected polymer film; and the thickness of the benzimidazole and imine connection polymer film is 20-2000nm. The method is applied to separation of mixtures such as methanol / dimethyl carbonate, methanol / toluene, methanol / methyl tert-butyl ether and the like for the first time. And for methanol / dimethyl carbonate and methanol / methyl tert-butyl ether, the mass fractions of methanol in the penetrating fluid can respectively reach 98.8% and 99.9% or above.

The utility model discloses a methyl tert -butyl ether sampling detection device relates to methyl tert -butyl ether technical field, including the extraction pipe, the surface fixedly connected with the pivot of extraction pipe, the surface of extraction pipe is opened with the square hole, the fixedly connected with the discharge gate of extraction pipe surface square hole department, the outside of pivot is inserted with the connecting shaft, the outside rotation is connected with the collection box of connecting shaft, the surface fixedly connected with the baffle of collection box. The utility model through baffle divides the inside of collection box into several cabins, through with the specified cabin corresponding mark rotates to the display hole department, then will sample liquid extraction to the inside of extraction pipe, then will device upset, then to the one side rotation limit rod of fixed plate arc shape, through the spring's elasticity, will piston rod push back, and stop moving at the front side of spacing septum, and the sample of extraction flows into the specified cabin inside due to the action of gravity, then rotates the knob to the next mark department again, thereby realizes the sample of multiple extraction and separates storage.

The invention discloses a membrane aeration bioreactor device for removing methyl tert-butyl ether pollution in high-salinity underground water. Comprising an external methyl tert-butyl ether polluted underground water conveying device, a soil sample chamber, a CDI (capacitive deionization) material, a biological function chamber, a dissolved oxygen intelligent control system, a pH intelligent control system, a methyl tert-butyl ether degrading bacterium supply device, a filter material chamber and a purified underground water collection device which are connected in sequence. The underground water polluted by the methyl tert-butyl ether flows into the soil sample chamber through the conveying device, and the soil sample chamber is filled with the soil polluted by the methyl tert-butyl ether to simulate the site environment. Groundwater gradually penetrates through the CDI material through soil pores and enters the biological function chamber, in the CDI material, anions and cations in the underground water move towards electrodes at the two ends under the action of an electric field, a deionization area is formed in the middle to discharge water, and electron donors needed by microorganisms are provided while salinity is removed.

The invention discloses a synthesis and purification method of a nalbuphine intermediate, the synthesis route of the method is as shown in the specification, and the method comprises the following steps: (1) in a reaction solvent, carrying out oxidation reaction on a compound I and peroxide to generate a compound II; (2) quenching, concentrating and extracting the reaction liquid containing the compound II obtained in the step (1), concentrating an extracted organic phase, adding a recrystallization solvent, and recrystallizing at-5 DEG C to 30 DEG C to obtain the compound II; wherein the recrystallization solvent is a mixed solvent of methyl tert-butyl ether and a low-polarity solvent, and the volume ratio of the methyl tert-butyl ether to the low-polarity solvent is (1-9): (1-4). According to the method, the purity of the compound II and the recrystallization yield are remarkably improved, side reactions in the subsequent nalbuphine synthesis process can be reduced, the cost is reduced, and industrial production is facilitated.

This invention discloses a solvate of rememega-2 and its preparation method. The rememega-2 solvate (compound II) provided by this invention has a purity of up to 99.15% compared with the existing rememega-2 methyl tert-butyl ether solvate. The preparation method includes the following steps: adding crude rememega-2 to a binary solvent, heating to 45℃~75℃, adding an antisolvent, stirring, crystallizing, cooling and filtering, and drying under reduced pressure to obtain rememega-2 solvate (compound II). Then, placing rememega-2 solvate (compound II) in ethanol and stirring to obtain rememega-2 (compound I). This route is simple to operate, the process is stable, and it is easy to scale up. Moreover, the rememega-2 (compound I) prepared by this solvate (compound II) has a purity of up to 99.66%, which is higher than that of rememega-2 (compound I) prepared by the existing rememega-2 methyl tert-butyl ether solvate. It also has better fluidity, does not caking, is easy to transfer, and is convenient for later production.

The present invention discloses a pleuromutilin derivative containing a 2-thio-4-thiazolidinone side chain, and its preparation method and application. The preparation method is as follows: first, pleuromutilin and p-toluenesulfonyl chloride are added to methyl tert-butyl ether, sodium hydroxide solution is added dropwise with stirring, and the reaction is refluxed to obtain intermediate 1; then, 2-thio-4-thiazolidinone is added to acetonitrile, anhydrous potassium carbonate is added and stirred at room temperature for 20 minutes, and then intermediate 1 is added, and the reaction is stirred at room temperature for a certain time to obtain intermediate 2; finally, intermediate 2 and an acyl chloride with different group donors are added to dichloromethane, and under the catalysis of triethylamine, stirred at room temperature for a certain time to obtain a pleuromutilin derivative containing a 2-thio-4-thiazolidinone side chain. The present invention synthesizes a pleuromutilin derivative containing a 2-thio-4-thiazolidinone side chain with good antibacterial activity through a simple preparation process, with high yield, good antibacterial activity against drug-resistant bacteria, and good application prospects.

The invention discloses a self-assembled fluorescent probe as well as a preparation method and application thereof. The preparation method comprises the following steps: adding 1-ethyl-2-methylbenzo [cd] indole-1-onium into a mixed solution of 10-[4-(dimethylamino) phenyl]-7-phenyl-3, 11-dithia-7-aza-tricyclo [6.3. 0.02, 6] undec-1 (8), 2 (6), 4, 9-tetraene-4-formaldehyde, acetic acid, acetic anhydride and triethylamine, and then mixing with methyl tert-butyl ether, so as to obtain the small molecule DMA-IN3, and carrying out ultrasonic self-assembly to obtain the nano-particle DINP. The self-assembled fluorescent probe DINP obtained by the invention has fluorescence property and phototoxicity, can be enriched to tumor tissues in a targeted manner, generates a large amount of heat under an illumination condition, realizes visual diagnosis and treatment of tumors, is good in biological safety, and provides a new scheme for clinical tumor treatment.

The purpose of the present invention is to provide a means for obtaining SMTP from a fermentation product including SMTP via a method that can easily be scaled up; to provide an SMTP crystallization method; or to provide a means for obtaining SMTP via a method including SMTP crystallization. The present invention provides an SMTP production method including: a step I for putting a Stachybotrys microspora fermentation product in contact with an aqueous solvent having a pH of 9 or greater and obtaining an extract liquid including SMTP; a step II for supplying the extract liquid obtained in step I for purification and obtaining a purified product; and a step III for crystallizing the SMTP included in the purified product obtained in step II in a crystallization solvent including one or more selected from the group consisting of ethyl acetate, butyl acetate, acetone, 2-butanone, diisopropyl ether, diethyl ether, methyl-tert-butyl ether, and acetonitrile. Alternatively, provided is an SMTP production method including a step for crystallizing SMTP having a purity of 70% or greater in a solvent including one or more selected from the group consisting of ethyl acetate, butyl acetate, acetone, 2-butanone, diisopropyl ether, diethyl ether, methyl-tert-butyl ether, and acetonitrile. Alternatively, provided is an SMTP production method including putting a Stachybotrys microspora fermentation product in contact with an aqueous solvent having a pH of 9 or greater and obtaining an extract liquid including SMTP.

The invention provides a preparation method of annomeline tartrate crystal form A. Cu-K alpha radiation is used, and an X-ray powder diffraction pattern of the crystal form A expressed by a 2 theta angle has characteristic peaks at 9.4 degrees + / -0.2 degrees, 20.4 degrees + / -0.2 degrees and 23.7 degrees + / -0.2 degrees. The preparation method comprises the following steps: (1) adding annomeline and L-tartaric acid into a first organic solvent, and stirring to obtain a second organic solvent; stirring and heating to obtain a clear solution; (2) adding a second organic solvent into the settled solution in the step (1), slowly cooling to 2-8 DEG C while stirring, and separating out crystals; and (3) filtering and collecting crystals, washing with a second organic solvent, and carrying out vacuum drying to obtain the Nomeline tartrate crystal form A. The first organic solvent is selected from methanol, ethanol, isopropanol, n-butanol, acetone or a combination thereof, and the second organic solvent is selected from methyl tert-butyl ether, an ester solvent, acetonitrile, an alkane solvent or a combination thereof. The preparation method of the xanthomeline tartrate is simple to operate, high in yield and high in purity.