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Chiral analysis bonded phase chromatography-tandem mass spectrometry method for nicotine in tobacco juice

A technology of electronic cigarette liquid and convergence chromatography, which is applied in the direction of analyzing materials, material separation, measuring devices, etc., can solve the problems of complex processing steps and inability to meet the detection of a large number of samples, achieve high sensitivity, good specificity, and avoid false positives Effect

Active Publication Date: 2017-06-27
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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Problems solved by technology

[0003] At present, the chiral analysis of nicotine is mainly studied by gas chromatography. For example, the patent application number 201610663534.1 "A Method for Chiral Analysis of Nicotine in Cigarette Tobacco" has complicated pretreatment steps and a long detection time. Up to 140min, which cannot satisfy the detection of a large number of samples. At present, there is no method for chiral analysis of nicotine in e-cigarette liquid

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  • Chiral analysis bonded phase chromatography-tandem mass spectrometry method for nicotine in tobacco juice
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  • Chiral analysis bonded phase chromatography-tandem mass spectrometry method for nicotine in tobacco juice

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Embodiment Construction

[0015] The present invention is further described by the following specific examples, but does not limit the present invention.

[0016] 1. Instruments and Reagents

[0017] ACQUITY UPC2 ultra-high performance convergence chromatograph (Waters, USA), 6410B triple quadrupole mass spectrometer (Agilent, USA), AE163 electronic balance (sensitivity: 0.0001 g, Mettler, Switzerland), high-speed pulverizer (Wuhan Yincai Technology Co., Ltd.), ColeparmerVotex-Genie vortex oscillator in the United States, and SIGMA 3-30K-high-speed desktop refrigerated centrifuge in Germany. MTBE (chromatographically pure), sodium hydroxide (analytical pure), formic acid (chromatographically pure), methanol (chromatographically pure), S-(-)-nicotine standard (CAS: 54-11-5 ), R-(+)-nicotine standard (CAS: 25162-00-9).

[0018] 2. Preparation of standard solution

[0019] Weigh about 100.0 mg of S-(-)-nicotine standard substance, dilute it with methanol solution to a 50 mL brown volumetric flask, and ...

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Abstract

The invention discloses a chiral analysis bonded phase chromatography-tandem mass spectrometry method for nicotine in tobacco juice. The method is characterized by comprising the following steps: infiltrating an electronic tobacco juice sample with a sodium hydroxide solution, extracting by using a methyl tertiary butyl ether and methanol solution, performing matrix dispersion solid-phase extraction and purification on extract liquor, analyzing by using a tandem mass-bonded phase chromatography tandem mass spectrometry method with two chiral columns, and performing quantitative detection on the ratio of S-(-)-nicotine to R-(+)-nicotine in the electronic tobacco juice by using a peak area normalization method. By adopting the detection method disclosed by the invention, a sample extraction and purification method is simple and efficient, contamination of the sample liquid to chromatographic columns can be reduced, the bonded phase chromatography tandem mass spectrometry method is high in sensitivity, good in specificity and short in analysis time, false positive can be excluded, and chiral analysis on the nicotine in large scales of electronic tobacco juice samples can be met.

Description

technical field [0001] The invention relates to a method for determining chemical components of electronic cigarette liquid, in particular to a chiral analysis convergence chromatography tandem mass spectrometry method for nicotine in electronic cigarette liquid. Background technique [0002] Nicotine, also called nicotine, is an alkaloid found in plants of the nightshade family and an important component of tobacco. The nicotine molecule contains a chiral center, which is the 2-position carbon atom on the tetrahydropyrrole ring, so nicotine has two enantiomers: S-(-)-nicotine and R-(+)-nicotine. The two enantiomers of nicotine have completely different contents, metabolic mechanisms and physiological properties. Therefore, chiral analysis of nicotine in e-cigarette liquid is required. [0003] At present, the chiral analysis of nicotine is mainly studied by gas chromatography. For example, the patent application number 201610663534.1 "A Method for Chiral Analysis of Nicot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/72
CPCG01N30/06G01N30/7266G01N2030/062
Inventor 张洪非费婷陈欢胡少东姜兴益庞永强李翔宇朱风鹏吴达戚大伟付亚宁罗彦波韩书磊
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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