A Convergence Chromatography Tandem Mass Spectrometry Method for Chiral Analysis of Nicotine in Jieyanling
A technology of convergence chromatography and tandem mass spectrometry, applied in the directions of analysis of materials, material separation, instruments, etc., can solve the problems such as nicotine in quit smoking spirit, can not meet the detection of a large number of samples, complex processing steps, etc., and achieve high sensitivity and specificity. Good sex, avoid false positive effect
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[0015] The present invention is further described by the following specific examples, but does not limit the present invention.
[0016] 1. Instruments and Reagents
[0017] ACQUITY UPC2 ultra-high performance convergence chromatograph (Waters, USA), 6410B triple quadrupole mass spectrometer (Agilent, USA), AE163 electronic balance (sensitivity: 0.0001 g, Mettler, Switzerland), high-speed pulverizer (Wuhan Yincai Technology Co., Ltd.), ColeparmerVotex-Genie vortex oscillator in the United States, and SIGMA 3-30K-high-speed desktop refrigerated centrifuge in Germany. MTBE (chromatographically pure), sodium hydroxide (analytical pure), formic acid (chromatographically pure), methanol (chromatographically pure), S-(-)-nicotine standard (CAS: 54-11-5 ), R-(+)-nicotine standard (CAS: 25162-00-9).
[0018] 2. Preparation of standard solution
[0019] Weigh about 100.0 mg of S-(-)-nicotine standard substance, dilute it with methanol solution to a 50 mL brown volumetric flask, and ...
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