Convergence chromatography-tandem mass spectrometry for chiral analysis of nicotine in smokeless tobacco products
A technology of smokeless tobacco and convergence chromatography, which is applied in the field of chemical composition determination of smokeless tobacco products, can solve the problems of complex processing steps, cannot meet the detection of a large number of samples, etc., achieves high sensitivity, good specificity, and avoids false positives Effect
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[0015] The present invention is further described by the following specific examples, but does not limit the present invention.
[0016] 1. Instruments and Reagents
[0017] ACQUITY UPC2 ultra-high performance convergence chromatograph (Waters, USA), 6410B triple quadrupole mass spectrometer (Agilent, USA), AE163 electronic balance (sensitivity: 0.0001 g, Mettler, Switzerland), high-speed pulverizer (Wuhan Yincai Technology Co., Ltd.), ColeparmerVotex-Genie vortex oscillator in the United States, and SIGMA 3-30K-high-speed desktop refrigerated centrifuge in Germany. Methyl tert-butyl ether (chromatographically pure), sodium hydroxide (analytical pure), formic acid (chromatographically pure), methanol (chromatographically pure), S-(-)-nicotine standard (CAS: 54-11-5) , R-(+)-nicotine standard (CAS: 25162-00-9).
[0018] 2. Preparation of standard solution
[0019] Weigh about 100.0 mg of S-(-)-nicotine standard substance, dilute it with methanol solution to a 50 mL brown vol...
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