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Convergence chromatography-tandem mass spectrometry for chiral analysis of nicotine in smokeless tobacco products

A technology of smokeless tobacco and convergence chromatography, which is applied in the field of chemical composition determination of smokeless tobacco products, can solve the problems of complex processing steps, cannot meet the detection of a large number of samples, etc., achieves high sensitivity, good specificity, and avoids false positives Effect

Active Publication Date: 2019-04-12
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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Problems solved by technology

[0003] At present, the chiral analysis of nicotine is mainly studied by gas chromatography. For example, the patent application number 201610663534.1 "A Method for Chiral Analysis of Nicotine in Cigarette Tobacco". Up to 140min, which cannot meet the detection of a large number of samples. At present, there is no chiral analysis method for nicotine in smokeless tobacco products

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  • Convergence chromatography-tandem mass spectrometry for chiral analysis of nicotine in smokeless tobacco products
  • Convergence chromatography-tandem mass spectrometry for chiral analysis of nicotine in smokeless tobacco products
  • Convergence chromatography-tandem mass spectrometry for chiral analysis of nicotine in smokeless tobacco products

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Embodiment Construction

[0015] The present invention is further described by the following specific examples, but does not limit the present invention.

[0016] 1. Instruments and Reagents

[0017] ACQUITY UPC2 ultra-high performance convergence chromatograph (Waters, USA), 6410B triple quadrupole mass spectrometer (Agilent, USA), AE163 electronic balance (sensitivity: 0.0001 g, Mettler, Switzerland), high-speed pulverizer (Wuhan Yincai Technology Co., Ltd.), ColeparmerVotex-Genie vortex oscillator in the United States, and SIGMA 3-30K-high-speed desktop refrigerated centrifuge in Germany. Methyl tert-butyl ether (chromatographically pure), sodium hydroxide (analytical pure), formic acid (chromatographically pure), methanol (chromatographically pure), S-(-)-nicotine standard (CAS: 54-11-5) , R-(+)-nicotine standard (CAS: 25162-00-9).

[0018] 2. Preparation of standard solution

[0019] Weigh about 100.0 mg of S-(-)-nicotine standard substance, dilute it with methanol solution to a 50 mL brown vol...

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Abstract

The invention discloses a chiral analysis bonded phase chromatography-tandem mass spectrometry for nicotine in a smokeless tobacco product. The chiral analysis bonded phase chromatography-tandem mass spectrometry comprises the following steps: drying, crushing and sieving a sample of a smokeless tobacco product; soaking the sample with a sodium hydroxide solution; extracting with methyl tert-butyl ether and methanol solution to obtain an extract; performing matrix solid-phase extraction and purification on the extract; analyzing with two chiral columns tandem bonded phase chromatography-tandem mass spectrometry; and quantitatively detecting S-(-)- nicotine and R-(+)- nicotine in the smokeless tobacco product by a peak area normalization method. A detection method provided by the invention adopts a simple and efficient sample extraction and purification method, and can reduce pollution of sample liquid on a chromatographic column; the bonded phase chromatography-tandem mass spectrometry detection method is high in sensitivity, good in specificity, capable of excluding false positive, short in analysis time, and capable of meeting chiral analysis for nicotine in mass samples of smokeless tobacco product.

Description

technical field [0001] The invention relates to a method for determining chemical components of smokeless tobacco products, in particular to a chiral analysis convergence chromatography tandem mass spectrometry method for nicotine in smokeless tobacco products. Background technique [0002] Nicotine, also called nicotine, is an alkaloid found in plants of the nightshade family and an important component of tobacco. The nicotine molecule contains a chiral center, which is the 2-position carbon atom on the tetrahydropyrrole ring, so nicotine has two enantiomers: S-(-)-nicotine and R-(+)-nicotine. The two enantiomers of nicotine have completely different contents, metabolic mechanisms and physiological properties. Therefore chiral analysis of nicotine in smokeless tobacco products (pouch snus) is required. [0003] At present, the chiral analysis of nicotine is mainly studied by gas chromatography. For example, the patent application number 201610663534.1 "A Method for Chiral...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/72
CPCG01N30/06G01N30/7266G01N2030/062
Inventor 庞永强费婷张洪非陈欢姜兴益罗彦波李翔宇戚大伟吴达朱风鹏胡少东付亚宁韩书磊
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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