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Method for efficiently extracting carotenoids in yellow peach fruits and determining carotenoids in yellow peach fruits by liquid phase

A carotene and liquid phase technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long time consumption, high cost, and complicated gradient elution process, and achieve simple operation, high accuracy, and short liquid phase time Effect

Inactive Publication Date: 2015-07-01
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mobile phase composition and gradient elution process of this method are complex, time-consuming and costly

Method used

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  • Method for efficiently extracting carotenoids in yellow peach fruits and determining carotenoids in yellow peach fruits by liquid phase
  • Method for efficiently extracting carotenoids in yellow peach fruits and determining carotenoids in yellow peach fruits by liquid phase
  • Method for efficiently extracting carotenoids in yellow peach fruits and determining carotenoids in yellow peach fruits by liquid phase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] a. The steps for preparing the standard solutions of 5 carotenoids are: accurately weigh 1mg of carotenoid standard products respectively, dissolve them in 10 mL of acetone to obtain a standard solution with a concentration of 100 mg / L; pipette 1.00 mg / L respectively Each standard solution in mL was mixed and diluted step by step with acetone to prepare a single standard solution of each concentration and a mixed standard solution of each concentration. Take the mixed standard solution that concentration is 10mg / L and obtain standard solution curve through high-performance liquid chromatography analysis, see figure 1 , the chromatographic conditions are:

[0049] Chromatographic column: YMC-C30;

[0050] Mobile phase: methyl tert-butyl ether: methanol = 30:70;

[0051] Elution method: isocratic elution;

[0052] Flow rate: 1.0 mL / min;

[0053] Wavelength: 450nm;

[0054] Column temperature: 25°C;

[0055] Injection volume: 20μL;

[0056] Time: 15 minutes;

[0057]...

Embodiment 2

[0061] Accurately weigh 0.5g of the ripe fruit pulp of the 'Jinxiu' variety, add 3mL of acetone, avoid light and ultrasonically extract for 1 h, centrifuge at 10,000 rpm for 10 min at 4°C, take the supernatant and filter it through a 0.22 mm organic filter head to obtain a sample solution for chromatography Column, chromatographic conditions are with embodiment 1, and the chromatogram of gained sample solution is shown in image 3 , the contents of various carotenoids in peach pulp are shown in Table 1.

Embodiment 3

[0063] Accurately weigh 0.5g of ripe fruit pulp of 'Ruiguang No. 20', add 3mL of acetone, avoid light and ultrasonically extract for 1 hour, centrifuge at 10,000 rpm for 10 minutes at 4°C, take the supernatant and filter it through a 0.22 mm organic filter head to obtain the sample solution Advance chromatographic column, chromatographic condition is with embodiment 1, and the chromatogram of gained sample solution is shown in Figure 4 , the contents of various carotenoids in peach pulp are shown in Table 1.

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Abstract

The invention discloses a method for efficiently extracting carotenoids in yellow peach fruits and determining the carotenoids in the yellow peach fruits by a liquid phase. Extracting conditions are as follows: the material-liquid ratio of pulp to an extracting agent acetone is 1 to 6 (g / mL), and ultrasonic light-shielding assisted extraction is carried out for 1 hour; chromatographic conditions are as follows: a YMC-C30 type chromatographic column is adopted; a mobile phase comprises methyl tert-butyl ether methanol with a ratio being 30 to 70; an isocratic elution manner is adopted; a flow speed is equal to 1.0mL / min; the wavelength is 450nm; the column temperature is 25 DEG C; a sample feeding amount is 20 microliters; and the time lasts for 18 minutes. According to the method for efficiently extracting the carotenoids in the yellow peach fruits and determining the carotenoids in the yellow peach fruits by the liquid phase, the extraction material-liquid ratio is 1 to 6 (g / mL), the ultrasonic light-shielding assisted extraction is carried out for 1 hour and the methyl tert-butyl ether and the methanol with the ratio of 30 to 70 are used as the mobile phase for carrying out the isocratic elution; the three conditions are combined so that a plurality of types of carotenoids in the yellow peach fruits can be effectively and completely extracted and rapidly and accurately determined; and the method is easy to operate, free of saponification and gradient elution, good in repeatability of a determined result, high in accuracy and short in liquid phase time.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis and instrumental analysis in the chemical industry. Background technique [0002] Yellow-fleshed peaches can be used fresh or stored in jars, and have high economic value and research value. Carotenoids are one of the important antioxidant substances in yellow-fleshed peaches. According to existing literature, the currently disclosed extraction methods for carotenoids in plants such as vegetables and fruits are cumbersome, often require saponification treatment, and the extraction effect is not good, and the conditions for high-performance liquid chromatography are complicated, and the mobile phase composition ratio used Both the gradient elution process and the gradient elution process are very complicated, the determination component is single, the determination time of the sample is long, and the cost is high. Therefore, in order to effectively evaluate and utilize yellow-fleshed pea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/34
Inventor 严娟沈志军蔡志翔张斌斌马瑞娟俞明亮
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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