Composition for pesticide, insecticide and application thereof

Abstract

The invention relates to a composition for a pesticide, an insecticide and application of the insecticide, the composition comprises emamectin benzoate and a bamboo vinegar diluent, and the bamboo vinegar diluent is obtained by diluting bamboo vinegar with water. The insecticide comprises the composition for the pesticide, and the insecticide is applied to prevention and treatment of lepidoptera pests, mites, coleoptera pests and homoptera pests, especially to prevention and treatment of plutella xylostella. In the composition disclosed by the invention, the bamboo vinegar diluent is used as a synergist to improve the pesticide effect of the emamectin benzoate, so that the pesticide containing the composition can improve the effect of preventing and treating lepidoptera pests, mites, coleoptera pests and homoptera pests, particularly has a remarkable effect of preventing and treating plutella xylostella, and further has a remarkable effect of preventing and treating the emamectin benzoate. According to the insecticide, the dosage of the emamectin benzoate can be reduced, the drug resistance of pests can be overcome and delayed, and meanwhile, the risk levels of environmental pollution, pesticide residues and the like can be reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticides, in particular to a pesticide composition, an insecticide and applications thereof. Background Art

[0002] The diamondback moth (Plutella xylostella L.) belongs to the family of Lepidoptera. It has a wide range of host plants, strong reproductive capacity, short generation cycle, and high resistance level. It has become one of the most harmful lepidopteran pests to cruciferous vegetable crops worldwide. Chemical control is an important way to prevent and control the diamondback moth. However, over-reliance on chemical pesticides will not only lead to increasingly serious resistance of the diamondback moth, but also cause environmental pollution and other problems. Summary of the invention

[0003] Based on this, it is necessary to provide a composition for pesticides, an insecticide and its application in response to the above problems. The insecticide with the composition has a significant effect in controlling lepidopteran pests, mites, coleoptera and homoptera pests, and can reduce the amount of avermectin benzoate used under the same control effect.

[0004] A composition for pesticides comprises emamectin benzoate and bamboo vinegar diluent, wherein the bamboo vinegar diluent is obtained by diluting bamboo vinegar liquid with water.

[0005] In one embodiment, the bamboo vinegar dilution is obtained by diluting bamboo vinegar liquid 50-200 times.

[0006] In one embodiment, the mass ratio of the bamboo vinegar dilution to the emamectin benzoate is 0.1:1-200:1.

[0007] In one embodiment, the bamboo vinegar dilution is obtained by diluting bamboo vinegar liquid 100 times, and the mass ratio of the bamboo vinegar dilution to the avermectin benzoate is 0.1:1-200:1.

[0008] In one embodiment, the bamboo vinegar dilution is obtained by diluting bamboo vinegar liquid 100 times, and the mass ratio of the bamboo vinegar dilution to the avermectin benzoate is 40:1-100:1.

[0009] In one embodiment, the bamboo vinegar dilution is obtained by diluting bamboo vinegar liquid 100 times, and the mass ratio of the bamboo vinegar dilution to the avermectin benzoate is 50:1.

[0010] A pesticide, comprising the composition for pesticide.

[0011] In one embodiment, the mass fraction of the emamectin benzoate in the insecticide is 0.5%-90.9%.

[0012] The invention discloses an application of the insecticide in preventing and controlling lepidopteran pests, mites, coleoptera and homoptera pests.

[0013] Emamectin benzoate is a microbial-derived low-toxic insecticide and acaricide, which has the characteristics of high activity, broad insecticide spectrum, good compatibility and long lasting effect. The insecticide containing emamectin benzoate can be used to prevent and control lepidopteran pests, mites, coleoptera and homoptera pests, etc. Further, in the composition of the present invention, the bamboo vinegar dilution neither produces cross-resistance with emamectin benzoate, but also can be used as a synergist to further improve the efficacy of emamectin benzoate. Therefore, the insecticide containing the composition can further improve the effect of preventing and controlling lepidopteran pests, mites, coleoptera and homoptera pests, especially the effect of preventing and controlling diamondback moth is remarkable, which is of great significance for the cultivation and pest control of cruciferous vegetables such as radish and cabbage.

[0014] Furthermore, under the same control effect, the insecticide of the present invention can reduce the dosage of emamectin benzoate, not only can effectively overcome and delay the drug resistance of lepidopteran pests, mites, coleoptera and homoptera pests, but also can reduce the risk level of environmental pollution and pesticide residues, and improve the safety to humans and non-target organisms. In addition, bamboo vinegar is a plant-derived synergist, which not only has a wide source, a simple extraction method, but also is easy to degrade, and has the significance of safety, environmental protection, reduced environmental pollution, and significant economic benefits and environmental protection. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the conventional technology, the drawings required for use in the embodiments or the conventional technology descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0016] Figure 1 This is the experimental design diagram for Example 2. DETAILED DESCRIPTION

[0017] For ease of understanding of the present invention, the present invention will be described in more detail below. However, it should be understood that the present invention can be implemented in many different forms and is not limited to the embodiments or examples described herein. On the contrary, the purpose of providing these embodiments or examples is to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0018] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific implementation methods or embodiments, and are not intended to limit the present invention. The optional scope of the term "and / or" used herein includes any one of two or more related listed items, and also includes any and all combinations of related listed items, and the combinations include any two related listed items, any more related listed items, or all related listed items.

[0019] In the present invention, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values ​​of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0020] In a first aspect, the present invention provides a composition for pesticides, comprising emamectin benzoate and bamboo vinegar diluent, wherein the bamboo vinegar diluent is obtained by diluting bamboo vinegar liquid with water.

[0021] Emamectin benzoate is a low-toxic insecticide and acaricide of microbial origin with the characteristics of high activity, broad insecticidal spectrum, good compatibility and long lasting effect. It can be used to control lepidopteran pests, mites, coleoptera and homopteran pests.

[0022] Bamboo vinegar is made from bamboo as raw material. It is obtained by condensing the gasification products produced during the burning process of bamboo charcoal. It contains nearly 300 natural high-molecular organic compounds such as γ-butyrolactone, 1-hydroxy-2-butanone, phenol, 3-methyl-1,2-cyclopentanedione (maple lactone), 2,5-dimethylfuran, guaiacol, and allyl butyrate.

[0023] Therefore, when the bamboo vinegar dilution is compounded with emamectin benzoate to form a composition, the bamboo vinegar dilution will not produce cross-resistance with emamectin benzoate, and can be used as a plant-based synergist to further improve the efficacy of emamectin benzoate in preventing and controlling lepidopteran pests, mites, coleoptera and homopteran pests, especially significantly improve the effect of preventing and controlling diamondback moth, and the mortality rate of diamondback moth is high.

[0024] Optionally, the bamboo vinegar dilution is obtained by diluting the bamboo vinegar liquid by 50-200 times, including but not limited to 50 times, 60 times, 70 times, 80 times, 90 times, 100 times, 110 times, 120 times, 130 times, 140 times, 150 times, 160 times, 170 times, 180 times, 190 times, 200 times, etc. The bamboo vinegar dilution obtained by diluting the bamboo vinegar liquid by 50-200 times has a more significant improvement in the efficacy of aminoavermectin benzoate in preventing and controlling lepidopteran pests, mites, coleoptera and homoptera pests.

[0025] Optionally, the mass ratio of the bamboo vinegar dilution to the emamectin benzoate is 0.1:1-200:1, including but not limited to 0.1:1, 0.5:1, 1:1, 2:1, 5:1, 10:1, 20:1, 30:1, 40:1, 50:1, 60:1, 70:1, 80:1, 90:1, 100:1, 110:1, 120:1, 130:1, 140:1, 150:1, 160:1, 170:1, 180:1, 190:1, 200:1, etc., and more preferably 40:1-200:1. When the mass ratio of the bamboo vinegar dilution to the emamectin benzoate is within this range, the efficacy of the bamboo vinegar dilution on emamectin benzoate in controlling lepidopteran pests, mites, coleoptera and homopteran pests is also more significantly improved.

[0026] Preferably, the bamboo vinegar dilution is obtained by diluting bamboo vinegar liquid 100 times, and the mass ratio of the bamboo vinegar dilution to the emamectin benzoate is 0.1:1-200:1. With such an arrangement, the synergistic ratio of the bamboo vinegar dilution to the efficacy of emamectin benzoate is above 1.33.

[0027] Further preferably, the bamboo vinegar dilution is obtained by diluting the bamboo vinegar liquid 100 times, and the mass ratio of the bamboo vinegar dilution to the emamectin benzoate is 40:1-100:1. With this arrangement, the synergistic ratio of the bamboo vinegar dilution to the efficacy of emamectin benzoate is above 2.78.

[0028] More preferably, the bamboo vinegar dilution is obtained by diluting the bamboo vinegar liquid 100 times, and the mass ratio of the bamboo vinegar dilution to the emamectin benzoate is 50:1. With this arrangement, the bamboo vinegar dilution has a better synergistic effect on the efficacy of emamectin benzoate, which can reach 5 or more.

[0029] The second aspect of the present invention provides an insecticide, which includes the composition for pesticide.

[0030] It is understood that the pesticide also includes agriculturally acceptable adjuvants, including but not limited to dispersants, wetting agents, thickeners, defoamers, disintegrants, binders, colorants, stabilizers, pigments, preservatives, etc., which can be selected and adjusted as needed. The pesticide can be prepared into any agriculturally acceptable dosage form, including emulsifiable concentrates, microemulsions, suspensions, water dispersible granules, wettable powders, suspoemulsions, seed coatings, extruded granules, coated granules, aerosols, aqueous emulsions, microcapsule suspensions, etc.

[0031] The pesticide of the present invention can be applied in a manner selected according to the target object and the use environment, including applying the pesticide of the present invention in an agronomically effective and substantially non-phytotoxic amount to the leaves, stems, and seeds, such as spraying, soaking, dusting, broadcasting, seed dressing, pouring, irrigation, drip irrigation, etc. The application amount may vary depending on the combination ratio of the active ingredients, meteorological conditions, the form of the agent, the application period, the application method, the application site, the control object, etc.

[0032] Since the bamboo vinegar dilution in the composition of the present invention neither produces cross-resistance with emamectin benzoate nor can serve as a synergist to further improve the efficacy of emamectin benzoate, the insecticide of the present invention having the composition can further improve the effect of preventing and controlling lepidopteran pests, mites, coleoptera and homoptera pests, especially has a significant effect of preventing and controlling diamondback moth, and has important significance for the cultivation and pest control of cruciferous vegetables such as radish and cabbage.

[0033] In the existing insecticides, the mass fraction of emamectin benzoate is above 1%. In the present invention, since the bamboo vinegar diluent can play a synergistic role, the mass fraction of emamectin benzoate in the insecticide can be as low as 0.5%. Specifically, the mass fraction of emamectin benzoate in the insecticide can be 0.5%-90.9%, preferably 0.5%-10%.

[0034] Thus, at the same content, the insecticide of the present invention is more effective in preventing and controlling lepidopteran pests, mites, coleoptera and homoptera pests. At the same control effect, the insecticide of the present invention can reduce the dosage of emamectin benzoate, which can not only effectively overcome and delay the drug resistance of lepidopteran pests, mites, coleoptera and homoptera pests, but also reduce the risk level of environmental pollution and pesticide residues, and improve the safety to humans and non-target organisms. Therefore, it is further preferred that the mass fraction of emamectin benzoate in the insecticide is 1%-2.5%.

[0035] In addition, bamboo vinegar is a plant-based enhancer, and its raw material is bamboo. It not only has a wide source, a simple extraction method, and low cost, but is also easily degradable. It is safe and environmentally friendly, reduces environmental pollution, and has significant economic benefits and environmental protection significance.

[0036] The third aspect of the present invention also provides a use of the insecticide in preventing and controlling lepidopteran pests, mites, coleoptera and homoptera pests.

[0037] In particular, the insecticide is used to control diamondback moth, with a high mortality rate and significant effect.

[0038] Hereinafter, the composition for pesticides, the insecticide and the application thereof will be further described through the following specific examples.

[0039] Example 1

[0040] 1. Test insects

[0041] The diamondback moth (Plutella xylostella) was a sensitive strain raised in the laboratory of Anhui Agricultural University. Fresh radish seedlings and artificial diet were used as food sources, and bamboo vinegar was diluted with water. The breeding temperature was maintained at 25±1℃, the humidity was 65%-75%; the light conditions were L:D=16h:8h. After several generations of breeding, healthy diamondback moth larvae with the same size and age (3rd instar) were selected as test insects.

[0042] 2. Experimental methods

[0043] The toxicity level of emamectin benzoate to the third instar larvae of Plutella xylostella was determined by feed mixing method. The emamectin benzoate original drug was prepared into a 10 g / L mother solution using acetone, and then the mother solution was gradiently diluted to five concentrations of 150 mg / L, 100 mg / L, 50 mg / L, 10 mg / L and 5 mg / L using 0.1% Tween 80 water. Bamboo vinegar was diluted to 50 times, 100 times and 200 times with water to prepare bamboo vinegar dilutions. The mother solution, bamboo vinegar dilutions and artificial feed were fully mixed according to the mass ratio of 1:1:100. After mixing, the concentration of emamectin benzoate in the feed was further diluted to 1.5 mg / L, 1 mg / L, 0.5 mg / L, 0.1 mg / L and 0.05 mg / L. The test insects were treated with the mixed feed, and the same amount of acetone and bamboo vinegar was mixed as a control. After starvation for 4 hours, the feed was placed in a 90 mm × 15 mm culture dish, and 12 third-instar larvae of Plutella xylostella were placed in each dish. Each concentration was repeated 3 times. After 48 hours of treatment, the mortality of the test insects was observed and counted. The criterion for judging the death of the test insects was: Use a brush to touch the insect body lightly, and it was considered dead if it could not crawl normally.

[0044] Synergistic effect evaluation method. Preparation of the test agent: dilute bamboo vinegar 100 times with water, mix the prepared 100-fold bamboo vinegar dilution with emamectin benzoate at a mass ratio of 0.1:1, 1:1, 10:1, 20:1, 50:1, 100:1, and 200:1 and add it to 20g of feed, and dilute emamectin benzoate into a series of gradient concentrations of 1.5mg / L, 1mg / L, 0.5mg / L, 0.1mg / L and 0.05mg / L. Treat the test insects with the mixed feed, and use the same amount of acetone bamboo vinegar as a control. After starvation for 4 hours, place the feed in a 90mm×15mm culture dish, and inoculate 12 third-instar larvae of Plutella xylostella in each dish, and repeat each concentration 3 times. Observe and count the mortality of the test insects after 48 hours of treatment. The criterion for judging the death of test insects: Use a brush to lightly touch the insect body; if it cannot crawl normally, it is considered dead.

[0045] 3. Calculation formula

[0046] SPSS21.0 software was used to perform toxicity regression analysis with the logarithm of drug concentration as the horizontal axis and the corrected mortality probability value as the vertical axis to calculate the toxicity regression equation, correlation coefficient r and half lethal concentration LC50.

[0047]

[0048] Among them, a synergistic ratio = 1 indicates no synergistic effect; a synergistic ratio > 1 indicates a synergistic effect; and a synergistic ratio < 1 indicates an antagonistic effect.

[0049] 4. Experimental results

[0050] The results of the indoor evaluation of the synergistic effect of bamboo vinegar dilution on avermectin benzoate in controlling Plutella xylostella are shown in Tables 1 and 2.

[0051] Table 1: Preliminary evaluation results of the synergistic effect of bamboo vinegar on emamectin benzoate in controlling Plutella xylostella

[0052]

[0053]

[0054] Note: The lowercase letters in the figure represent the differences between different treatment groups at the same concentration.

[0055] Table 2: The results of the synergistic effect of 100-fold bamboo vinegar dilution on emamectin benzoate

[0056]

[0057] As shown in Table 1, when the dilution ratio of bamboo vinegar is 100 times, it has a certain synergistic effect on the control of 3rd instar larvae of diamondback moth. When the test concentration of emamectin benzoate is 1 mg / L, the insecticidal activity of emamectin benzoate against diamondback moth larvae for 48 hours can be increased by about 10% when bamboo vinegar is diluted 100 times.

[0058] As shown in Table 2, when the mass ratio of 100-fold bamboo vinegar dilution to emamectin benzoate is 0.1:1, 1:1, 10:1, 20:1, 50:1, 100:1 and 200:1, the LC50 of the 100-fold bamboo vinegar dilution to the 3rd instar larvae of Plutella xylostella is 0.250 mg / L, 0.270 mg / L, 0.269 mg / L, 0.212 mg / L, 0.066 mg / L, 0.080 mg / L and 0.141 mg / L, respectively, and the synergistic ratios are 1.43, 1.33, 1.33, 1.69, 5.43, 4.49 and 2.55, respectively. With the increase of the mass ratio, the synergistic effect shows a trend of first increasing and then decreasing. Among them, when the mass ratio is 50:1, the synergistic effect of the bamboo vinegar dilution is the most significant, with an LC50 of 0.066 mg.

[0059] Example 2

[0060] Field application evaluation of bamboo vinegar on the synergistic effect of emamectin benzoate in controlling Plutella xylostella.

[0061] 1. Test crops

[0062] Shanghai blue.

[0063] 2. Test time and location

[0064] Test time: Control will be carried out when Shanghai green grows the third true leaf (March 2, 2024).

[0065] Experimental location: Greenhouse on the campus of Anhui Agricultural University.

[0066] Cultivation conditions: The selected plots are deeply plowed and then evenly sown with Shanghai green seeds after drying. Water is irrigated until the land is saturated, and normal water and fertilizer management is given during this period.

[0067] 3. Plot setting and experimental design

[0068] The experimental design is shown in Figure 1 There are 9 plots in total, each with an area of ​​1m 2 (1m×1m), with an interval of 0.5m, and random block arrangement. During the experiment, each plot was 1m 3 The plots were covered with bottomless insect-proof hoods (1m×1m×1m) to prevent insects from moving between plots. The soil was loam with moderate fertility, the site was flat, and irrigation was convenient. The cultivation and water and fertilizer management conditions of the experimental plots were consistent.

[0069] 4. Survey methods for the occurrence of diamondback moth

[0070] According to the relevant contents of GBT 17980.13-2000 Pesticide Field Efficacy Test Guidelines (I) Insecticides for Control of Lepidoptera Larvae on Cruciferous Vegetables, the number of live larvae on all plants in each plot was investigated. The number of live larvae on all plants in each plot was investigated once before application and 1, 3, 7, and 14 days after application, and the insect population reduction rate was calculated. The control effect was calculated based on the insect population reduction rate.

[0071]

[0072] 5. Experimental results

[0073] The control effect of 100-fold bamboo vinegar dilution and avermectin benzoate on Plutella xylostella is shown in Table 3.

[0074] Table 3: Field efficacy results of 100-fold bamboo vinegar dilution on emamectin benzoate against diamondback moth

[0075]

[0076] Note: Treatment group 1 was 5.7% emamectin benzoate suspension 18 mg, treatment group 2 was 100-fold bamboo vinegar dilution 45 mg + 5.7% emamectin benzoate suspension 18 mg, and treatment group 3 was clear water control; lowercase letters in the table indicate the differences between treatment groups at different times after the same medication.

[0077] As shown in Table 3, 100 times dilution of bamboo vinegar has a synergistic effect on 5.7% emamectin benzoate suspension in the control of diamondback moth in the field. The insect population reduction rate and control effect can be increased by about 10% after 1 day, and the insect population reduction rate is increased by 12.78% after 14 days, and the control effect is increased by 13.49%. The synergistic effect is significant. In agricultural production, 100 times dilution of bamboo vinegar can be used as a synergist of emamectin benzoate.

[0078] In summary, the bamboo vinegar dilution of the present invention can significantly improve the efficacy of emamectin benzoate, significantly increase the toxicity under the same medication conditions, reduce the dosage of emamectin benzoate, not only overcome and delay the drug resistance of pests, but also reduce the risk levels of environmental pollution and pesticide residues.

[0079] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0080] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A composition for pesticide, characterized in that: The composition comprises emamectin benzoate and bamboo vinegar diluent, wherein the bamboo vinegar diluent is obtained by diluting bamboo vinegar liquid with water.

2. The composition for pesticide according to claim 1, characterized in that The bamboo vinegar dilution is obtained by diluting bamboo vinegar liquid by 50-200 times.

3. The composition for pesticide according to claim 2, characterized in that: The mass ratio of the bamboo vinegar dilution to the emamectin benzoate is 0.1:1-200:

1.

4. The composition for pesticide according to claim 3, characterized in that The bamboo vinegar dilution is obtained by diluting bamboo vinegar liquid by 100 times, and the mass ratio of the bamboo vinegar dilution to the emamectin benzoate is 0.1:1-200:

1.

5. The composition for pesticide according to claim 4, characterized in that The bamboo vinegar dilution is obtained by diluting bamboo vinegar liquid by 100 times, and the mass ratio of the bamboo vinegar dilution to the emamectin benzoate is 40:1-100:

1.

6. The composition for pesticide according to claim 5, characterized in that: The bamboo vinegar dilution is obtained by diluting bamboo vinegar liquid 100 times, and the mass ratio of the bamboo vinegar dilution to the avermectin benzoate is 50:

1.

7. An insecticide, characterized in that: The insecticide comprises the composition for pesticide according to any one of claims 1 to 6.

8. The insecticide according to claim 7, characterized in that The mass fraction of the emamectin benzoate in the insecticide is 0.5%-90.9%.

9. Use of the insecticide according to claim 7 or 8 in controlling lepidopteran pests, mites, coleoptera and homoptera pests.

10. The use according to claim 9, characterized in that: The insecticide is used for preventing and controlling the diamondback moth.

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