Chemical dyeing method for differentiating diffuse type pine wood nematode
A pine wood nematode and chemical dyeing technology, which is applied in the field of chemical dyeing for identifying diffusive pine wood nematodes, can solve the difficulty in counting the ratio of reproductive and diffusing nematodes, the difficulty in distinguishing reproductive and diffusing nematodes, and the complicated operation of biochemical identification methods. and other problems, to achieve the effect of simple and reliable operation, easy purchase and stable structure
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Embodiment 1-3
[0022] Stimulate 1.5 μg dissolved in 20 μL of dimethyl sulfoxide (DMSO) according to the weight ratio listed in Table 1, then add 100 μL of the aqueous solution containing B. xylophilus and add 10 μL of 0.2% brillant-blue P-250 (Coomassie Brilliant Blue) was placed at room temperature for 24 hours (each treatment was repeated 10 times); and the observed staining effects were listed in Table 1.
[0023] Diffuse pine wood nematodes were treated and the staining effect was observed. Listed in Table 1. Each treatment was repeated 10 times.
[0024] α-pinene
Embodiment 4-6
[0026] Stimulate 0.5 μg of triethylene glycol diacetate dissolved in 20 μL of triethylene glycol diacetate according to the weight ratio listed in Table 2, then add 100 μL in the aqueous solution containing pine xylophilus and add 10 μL of 0.2% brillant-blueP-250 (library Marcy Brilliant Blue) was placed at room temperature for 24 hours (each treatment was repeated 10 times); and the observed staining effects were listed in Table 2.
[0027] Diffuse pine wood nematodes were treated and the staining effect was observed. Listed in Table 2. Each treatment was repeated 10 times.
[0028] α-pinene
Embodiment 7-9
[0030] Stimulant 10.5 μg dissolved in 20 μL of dichloromethane according to the weight ratio listed in Table 3, then added to 100 μL of the aqueous solution containing pine wood nematode and added 10 μL of 0.2% brillant-blue P-250 (Coomassie brilliant blue) Place at room temperature for 24 hours (10 repetitions per treatment); and the observed staining effects are listed in Table 3.
[0031] Diffuse pine wood nematodes were treated and the staining effect was observed. Listed in Table 3. Each treatment was repeated 10 times.
[0032] α-pinene
[0033] It can be seen from the table that using the chemical staining method provided by the present invention for identifying diffuse pine wood nematodes has a good identification effect, and provides a new method for the quarantine and investigation of pine wood nematodes in my country.
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