Nucleic aptamer-based organic arsenic molecular imprinting polymer in marine products, preparation method thereof and application thereof

Abstract

The invention discloses a nucleic aptamer-based organic arsenic molecular imprinting polymer in marine products, a preparation method thereof and application thereof. The molecular imprinting polymer is prepared by polymerizing organic arsenide template molecules, a functional monomer, a cross-linking agent, a pore-forming agent, an initiator and an organic solvent in a molar ratio of (0.1-2.5):3.0:(0.1-4):(30-60):(0.01-0.10):(1.0-10), wherein the functional monomer is a nucleic aptamer. The method for preparing the organic arsenic molecular imprinting polymer comprises the following steps of: (1) mixing the organic arsenide template molecules, the functional monomer, the cross-linking agent, the pore-forming agent, the initiator and the organic solvent uniformly in a molar ratio, and polymerizing; (2) taking the obtained polymer out of a reactor, grinding and sieving; and eluting by using eluent until the polymer does not contain bacterial toxins, cleaning by using the organic solvent until the solution is neutral, and drying under vacuum to obtain the molecular imprinting polymer. The molecular imprinting polymer has the excellent performance of molecular recognition, so that the sensitivity and selectivity of detecting organic arsenic in the marine products are improved greatly.

Description

technical field [0001] The invention relates to a seafood detection technology, in particular to a nucleic acid aptamer-based organic arsenic molecularly imprinted polymer in seafood that has the ability to specifically identify organic arsenic in seafood as a template molecule and a preparation method thereof; It also relates to the application of nucleic acid aptamers as functional monomers in the preparation of organic arsenic molecularly imprinted polymers in seafood. Background technique [0002] Arsenic contamination of seafood has always been the focus of attention in the field of food safety. Unlike terrestrial organisms, marine organisms generally have a strong ability to accumulate arsenic. Fish, shellfish, seaweed and other seafood that humans often eat contain high concentrations of arsenic. Arsenic in seafood mainly exists in the form of organic arsenic (such as methylarsinic acid, methylarsinic acid, arsenic sugar, arsenocholine, etc.), accounting for about 95...

AI Technical Summary

Problems solved by technology

Among them, chromatography technology can accurately separate organic arsenic in seafood, but the detection sensitivity is not satisfactory; mass spectrometry and coupled techniques (such as gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, etc.) The content of various organic arsenic in the product, but the instruments required by these two methods are expensive, and the sample pretreatment is extremely complicated, so the practicability is not high