The invention discloses an environment-friendly spinning refining agent for mulberry silks, and a preparation method of the refining agent. The environment-friendly spinning refining agent is prepared from the following raw materials in parts by weight: 10-20 parts of C16 secondary alkyl sodium sulfonate, 10-15 parts of carboxyethyl thiosuccinic acid, 5-12 parts of nonylphenol ethoxylate, 5-10 parts of acetic acid vinyl ester, 1-3 parts of glucose, 3-7 parts of glycerinum, 2-6 parts of sodium aminosalicylate, 1-4 parts of sulfonated succinate diamide salt, 2-5 parts of malic acid, 2-4 parts of potassium sorbate, 1-3 parts of cellulase, 1-4 parts of Double palmitoyl ethylenediamine dipropionate, 1-5 parts of trimethylolpropane, 1-2 parts of sodium tripolyphosphate, 1-3 parts of an anionic surfactant, 2-4 parts of an antifoaming agent, and 1-4 parts of an emulsifying agent. The prepared refining agent has good emulsion and dispersion performances, is small in stimulation to silk textures, and has the function of softening textures. In addition, the invention also discloses a corresponding preparation method.