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Method for screening trichoderma asperellum and application thereof

A screening method, Trichoderma technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve problems such as limited laboratory research stages, and achieve the effect of ensuring normal growth

Inactive Publication Date: 2013-01-16
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no domestic reports on fungi capable of bioaccumulating and volatilizing arsenic, and there are only a handful of related reports abroad, and most of them are limited to the stage of laboratory research

Method used

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  • Method for screening trichoderma asperellum and application thereof
  • Method for screening trichoderma asperellum and application thereof
  • Method for screening trichoderma asperellum and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Isolation and screening of embodiment 1 Trichoderma aculeatus

[0037] Collect arsenic-contaminated soil samples from arsenic-contaminated areas, isolate fungi from the soil samples in the laboratory, and inoculate them on the selective medium of Trichoderma after purification and culture, targeting the strain with the best growth status Bacterial strains were inoculated on solid-state PGP medium (potato-glucose-peptone medium) containing a certain concentration of arsenic, and their colony growth conditions were observed under different arsenic concentrations. At the same time, combined with the results of microscopic examination, they were screened out. Robust, arsenic-tolerant Trichoderma aculeatus.

[0038] Specifically include steps:

[0039] The above-mentioned arsenic-contaminated soil sample comes from the slag accumulation place near the realgar mine in Shimen area, Hunan Province. The soil sample was serially diluted and spread on the Martin medium with an ar...

Embodiment 2

[0045] Embodiment 2 Trichoderma aculeatus growth rate change experiment in different arsenic-containing solutions

[0046] Prepare PGP medium with arsenic content of 0, 10, 30, 50, 80, 100, 200mg / L respectively, and after sterilizing at 120-122°C for 14-16 minutes, 8-10mm Echinococcus sp. Trichoderma bacteria blocks were added to the above treatments, and cultured on a shaker at a temperature of 23-27°C and a rotational speed of 138-142rmp; after 5 days of cultivation, the culture solution was centrifuged at 3800-4200rmp for 8-12 minutes, and the suspension Count the number of spores in the solution; then wash the bacteria repeatedly with ultrapure water 4 times, wash off the residual culture medium, dry the bacteria at 48-52°C to constant weight, and then weigh the bacteria Mass weight, the biomass of Trichoderma aculeatus.

[0047] Such as figure 1 Shown is the biomass of Trichoderma aculeatus at the above-mentioned different levels of arsenic concentration (ie, arsenic co...

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Abstract

The invention discloses a method for screening trichoderma asperellum and application thereof. The method comprises the following steps of: collecting an arsenic pollution soil sample; separating fungi from the soil sample in a laboratory; purifying the fungi, and transferring the purified fungi to a selective culture medium of trichoderma; and culturing the fungi in different arsenic concentrations by taking a fungus strain of which the growth condition is the best on the selective culture medium as an object, and observing the growth condition of a bacterial colony and the tolerance of the bacterial colony on arsenic to screen the trichoderma asperellum.

Description

technical field [0001] The invention relates to a screening method for fungi and an application thereof, in particular to a screening method for Trichoderma asperellum (Trichoderma asperellum) and an application thereof. Background technique [0002] Arsenic is a toxic and carcinogenic non-metallic element that widely exists in nature. Arsenic pollution has become one of the most serious environmental problems in the world. According to reports, at least 50 million people in the world are facing the threat of endemic arsenic poisoning, most of them are Asian countries, and China is one of the countries most seriously affected by arsenic poisoning. There have been more than 30 arsenic poisoning incidents. my country is a big country with arsenic mines, and arsenic mines are widely distributed in Hunan, Yunnan, Guangxi, Guangdong and other provinces in the south-central and southwest of my country. The mining or smelting of arsenic mines, excessive input of arsenic-containing...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12Q1/02B09C1/10C12R1/885
Inventor 曾西柏苏世鸣蒋细良白玲玉李莲芳
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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