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Compound microbial soil remediation agent as well as preparation method and application thereof

A technology of composite microbial inoculum and soil remediation agent, applied in the field of soil remediation, to achieve the effect of good application prospects

Active Publication Date: 2018-08-24
江苏世邦生物工程科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many soil pollution remediation technologies that can be listed, there are few methods that can actually carry out soil pollution remediation

Method used

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  • Compound microbial soil remediation agent as well as preparation method and application thereof
  • Compound microbial soil remediation agent as well as preparation method and application thereof
  • Compound microbial soil remediation agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation method of composite microbial soil remediation agent is as follows:

[0022] 1) Preparation of Arthrobacter sp. LHM7719 bacterial agent:

[0023] Take Arthrobacter sp. LHM7719, place Arthrobacter sp. LHM7719 in a Erlenmeyer flask equipped with seed culture base, and carry out seed culture to the logarithmic phase under the conditions of pH 7.2 and 30°C, and then press Inoculate the strains cultivated to the logarithmic phase into LB medium at an inoculum size of 2% of the volume of the medium, pH 7.0, 30°C, shake on a shaker at 180rpm, and cultivate for 48 hours to obtain Arthrobacter sp. LHM7719 fermentation To the fermented liquid, add diatomite accounting for 30% of the mass of the fermented liquid, mix evenly, and dry to remove the moisture in the obtained mixture, which is Arthrobacter sp. LHM7719 microbial agent.

[0024] 2) Preparation of Bacillus subtilis LM3403 bacterial agent:

[0025] The activated Bacillus subtilis (Bacillus subtilis) LM340...

Embodiment 2

[0028] The preparation method of composite microbial soil remediation agent is as follows:

[0029] 1) Preparation of Arthrobacter sp. LHM7719 bacterial agent:

[0030] Take Arthrobacter sp. LHM7719, place Arthrobacter sp. LHM7719 in a Erlenmeyer flask equipped with seed culture base, and carry out seed culture to the logarithmic phase under the conditions of pH 7.2 and 30°C, and then press Inoculate the strains cultured to the logarithmic phase into LB medium at an inoculum size of 3% of the volume of the medium, pH 7.0, 35°C, shake on a shaker at 180rpm, and cultivate for 40 hours to obtain Arthrobacter sp. LHM7719 fermentation To the fermented liquid, add activated carbon accounting for 15% of the mass of the fermented liquid, mix evenly, and dry to remove the moisture in the resulting mixture, which is Arthrobacter sp. LHM7719 microbial agent.

[0031] 2) Preparation of Bacillus subtilis LM3403 bacterial agent:

[0032]The activated Bacillus subtilis (Bacillus subtilis) ...

Embodiment 3

[0035] The preparation method of composite microbial soil remediation agent is as follows:

[0036] 1) Preparation of Arthrobacter sp. LHM7719 bacterial agent:

[0037] Take Arthrobacter sp. LHM7719, place Arthrobacter sp. LHM7719 in a Erlenmeyer flask equipped with seed culture base, and carry out seed culture to the logarithmic phase under the conditions of pH 7.2 and 30°C, and then press Inoculate the strains cultivated to the logarithmic phase into LB medium at an inoculum size of 5% of the volume of the medium, pH 7.0, 35°C, shake at 180rpm on a shaker, and cultivate for 45 hours to obtain Arthrobacter sp. LHM7719 fermentation To the fermentation broth, add activated carbon accounting for 40% of the mass of the fermentation broth, mix evenly, and dry to remove the moisture in the resulting mixture, which is Arthrobacter sp. LHM7719 microbial agent.

[0038] 2) Preparation of Bacillus subtilis LM3403 bacterial agent:

[0039] The activated Bacillus subtilis (Bacillus sub...

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Abstract

The invention discloses a compound microbial soil remediation agent. The agent comprises an arthrobacter sp. LHM7719 agent, a Bacillus subtilis microbial agent and compound enzyme, wherein the compound enzyme comprises glyceraldehyde-3-phosphate dehydrogenase and beta-amylase. The invention also discloses a preparation method and an application of the compound microbial soil remediation agent. Thearthrobacter sp. LHM7719 is applied to the soil remediation field and can significantly reduce the content of heavy metals including arsenic, lead and cadmium in soil after being matched with Bacillus subtilis and glyceraldehyde-3-phosphate dehydrogenase and efficiently degrade petroleum hydrocarbon in the soil after being matched with the beta-amylase, so that the agent has good application prospects.

Description

[0001] Technical solutions [0002] The invention belongs to the technical field of soil remediation, and in particular relates to a composite microbial soil remediation agent and its preparation method and application. Background technique [0003] Soil is a resource that human beings depend on for survival and an important place for material and energy cycles in the ecosystem. Soil environment and ecological security are important guarantees for sustainable development. With the rapid development of the economy, the production and domestic wastewater, waste gas, waste residue and other wastes that have not been effectively treated have increased sharply. Many harmful substances have continuously entered the soil and accumulated. The overall situation of soil pollution is severe. Agricultural security and sustainable development have a greater impact. Among them, mining and metallurgy activities are one of the important reasons for excessive heavy metals in farmland and agr...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/06C12R1/125
CPCB09C1/10C12N1/20
Inventor 谢悦波姚竣耀刘才群
Owner 江苏世邦生物工程科技有限公司
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