13 results about "Ovary cell" patented technology

The application discloses a construction method and application of an ovary cell line of Quasipaa spinosa. The method comprises the following steps: selecting metamorphosis period larva ovary tissue, disinfecting and obtaining the ovary tissue, and then using collagenase I digestion solution to digest the ovary tissue at 26 DEG C for 1 hour; after the tissue block adheres to the wall, using a low-osmotic-pressure complete culture medium to carry out primary culture at 25-27 DEG C, wherein the culture medium is 60% diluted DMEM / F12 culture medium containing 10%-15% fetal bovine serum; when the cell confluence degree is higher than 70%, using trypsin-EDTA to digest the cells for 1-2 minutes to subculture, and the subculture culture medium contains 30% cell conditional culture medium; and finally, the cells are cryopreserved through programmed cooling. The ovary epithelial cell line of Quasipaa spinosa is successfully established for the first time, the survival rate of the cells after recovery is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and in-vitro conservation of germplasm resources.

The application discloses a kind of marker for predicting oocyte copy number variation and application thereof, the marker includes the combination of CLEC11A, P4HB, EFEMP2, IL32, FTL, FLNA, COL6A3, ACPP and APOO protein.By the marker of the application, the expression level of granulosa cell secretory protein can be determined by granulosa cell transcriptome sequencing, and the genomic CNV condition of corresponding oocyte can be inferred.The detection object of the technical solution is discarded granulosa cell in assisted reproductive technology, so it will not have any impact on oocyte, and it is non-invasive;The present study is a transcriptome and methylation group detection from single cell level, with high-throughput characteristics, and all granulosa cells and oocytes are one-to-one matched, which can reflect the difference between single cell level COCs.

The application relates to the field of biological fermentation, and discloses a traditional Chinese medicine fermentation composition for repairing ovary cells and a preparation method thereof. The traditional Chinese medicine fermentation composition is obtained by subjecting traditional Chinese medicine raw materials to microbial liquid fermentation; the traditional Chinese medicine raw materials include lilies, angelica, sea buckthorn, raspberries and medlar in a mass ratio of (1-2):(1-2):(1-2):(1-2):(1-2). The traditional Chinese medicine fermentation composition is obtained by mixing lilies, angelica, sea buckthorn, raspberries and medlar in a specific proportion and subjecting the mixture to biological liquid fermentation. The traditional Chinese medicine fermentation composition has extremely high antioxidant activity and excellent ovary cell repairing function.

The application belongs to the technical field of biology, and discloses a use of antisense nucleotide of piR-bmo-796514 nucleotide for preparing a medicine for inhibiting Bombyx mori nuclear polyhedrosis virus, the antisense nucleotide of the piR-bmo-796514 nucleotide is mainly used for inhibiting replication of the Bombyx mori nuclear polyhedrosis virus in ovary cells of the Bombyx mori, and a sequence of the piRNA nucleotide is shown as SEQ ID NO:1; in addition, the application also discloses the antisense nucleotide of the piR-bmo-796514 nucleotide, and an antisense nucleotide sequence is shown as SEQ ID NO:2.

The invention relates to the field of gene engineering, and discloses dsRNA of a bombyx mori glucose regulatory protein 78 gene and a preparation method of the dsRNA, and the preparation method comprises the following steps: a) designing and synthesizing an upstream primer and a downstream primer of a BmGRP78 gene; b) extracting bombyx mori total RNA, performing reverse transcription to obtain first chain cDNA, taking the first chain cDNA as a template, performing PCR amplification to obtain a BmGRP78 gene full-length fragment, purifying, cloning to escherichia coli, and performing sequencing verification; c) designing an upstream primer SEQ ID NO: 3 and a downstream primer SEQ ID NO: 4 of dsRNA according to the nucleotide sequence SEQ ID NO: 1 of the BmGRP78 gene, wherein both the upstream primer and the downstream primer carry a T7 promoter sequence; and d) in-vitro transcription synthesis of dsRNA which can specifically inhibit BmGRP78 gene expression and is used for bombyx mori to optimize silk production and improve disease resistance innovation. Compared with the prior art, the dsRNA has the effect of outstanding gene knock-down efficiency, and experimental results prove that the dsRNA can effectively reduce BmGRP78 gene expression in bombyx mori ovary cells (BmN) and silk glands.

The invention discloses a construction method and application of an ovarian cell line of hapalogenys nitens. The method comprises the following steps: selecting a metamorphosis stage larva ovarian tissue, disinfecting and obtaining, and digesting for 1 hour at 26 DEG C by using collagenase I digestive juice; after the tissue block adheres to the wall, a low-osmotic-pressure complete medium is used for primary culture at the temperature of 25-27 DEG C, and the medium is a 60% diluted DMEM / F12 medium containing 10%-15% of fetal calf serum; after the cell confluence degree exceeds 70%, trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) is used for digesting for 1-2 minutes for passage, and a passage culture medium contains 30% of a cell condition culture medium; and finally performing programmed cooling and cryopreservation. According to the method, the ovarian epithelial cell line of the hapalogenys nitens is successfully established for the first time, the cell recovery survival rate is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and germplasm resource in-vitro preservation.

The application discloses a flower glue peptide with ovary protection and whitening effects and a preparation method and application thereof. The preparation method comprises the following steps: uniformly mixing flower glue of a sea bass in a certain proportion, homogenizing the flower glue by using a homogenizer, sterilizing the flower glue by using a high-pressure sterilization pot, performing fermentation treatment on the flower glue by using Lactobacillus mucosus JIAN, adjusting the pH of the treated flower glue solution by using hydrochloric acid, adding acid protease of Aspergillus niger for enzyme hydrolysis, and then adding aminopeptidase for enzyme hydrolysis, so as to obtain a flower glue peptide solution. The flower glue peptide prepared by the application has significant ovary protection and whitening functions. The whitening function is mainly embodied in tyrosinase inhibition activity, and the ovary protection function is embodied in hydrogen peroxide-induced ovary cell survival rate and anti-mullerian hormone content, so that the economic value of the sea bass by-product is greatly improved. In addition, the flower glue peptide also has the characteristics of weak fishy smell, low fat content and small molecular weight.

The application provides a method for constructing a gene editing complex mutant mouse model, which comprises the following steps: injecting sgRNA, ssDNA and Cas9 protein with a nucleotide sequence as shown in SEQ ID NO. 1 into a mouse zygote cell, injecting sgRNA, Cas9 protein and a plasmid containing a nucleotide sequence as shown in SEQ ID NO. 12 into a mouse zygote cell, and respectively constructing gene editing zygotes; implanting the zygotes into the oviducts of pseudopregnant mice to obtain F0 generation mice; mating the single-gene F0 generation mice with wild-type mice to obtain single-gene F1 generation heterozygote mice; mating the GBA V394L point mutation and PSAP double-heterozygote positive mice or mating the GBA V394L point mutation homozygote and PSAP heterozygote positive mice, so as to obtain the modeling mice. The mice obtained by the method can better simulate the pathological characteristics of Gaucher disease and PD.

The invention discloses a high-precision egg cell / early embryo electric activation device. The device comprises a power supply; the electrical activation chip comprises a base and an electrode layer, the base is provided with a plurality of electrical activation grooves arranged at intervals and two opposite electrode pits communicated with the electrical activation grooves, the electrode layer comprises two electrode assemblies, each electrode assembly comprises an electrode part and a conductive part connected to the electrode part, the electrode parts are arranged in the electrical activation grooves, and the conductive parts are arranged in the electrical activation grooves. The two electrode parts and the electric activation groove form an electric activation chamber, and the conductive parts are arranged in the corresponding electrode pits; and the wire assembly is used for connecting the power supply with the two conductive parts of the electrically activated chip. The invention also discloses a high-precision egg cell / early embryo electric activation method. According to the invention, the accuracy and the stability of egg cell / early embryo electric activation are greatly improved, a stable and uniform electric field can be provided under a low-voltage condition, the voltage demand is reduced, the electric damage to cell membranes is reduced, and the cell survival rate and the activation efficiency are remarkably improved.

The preparation method comprises the following steps: uniformly mixing perch fish maw with water according to a certain proportion, homogenizing by using a homogenizer, sterilizing by using a high-pressure sterilization pot, carrying out fermentation treatment by using lactobacillus mucus JIAN, then adjusting the pH value of the treated fish maw solution by using hydrochloric acid, and carrying out freeze-drying to obtain the fish maw peptide with ovary protection and whitening effects. Aspergillus niger acid protease is added for enzymolysis, then aminopeptidase is used for enzymolysis, and a fish maw peptide solution is obtained. The fish maw peptide prepared by the invention has remarkable whitening and ovarian protection functions, the whitening function is mainly embodied from tyrosinase inhibitory activity, the ovarian protection function is embodied by measuring cell survival rate and anti-mullerian hormone content by inducing ovarian cells through hydrogen peroxide, and the economic value of perch byproducts is greatly improved. In addition, the fish maw peptide also has the characteristics of weak fishy smell, low fat content, small molecular weight and the like.

The invention provides a fermented dandelion composition with an ovarian cell repairing function as well as a preparation method and application thereof, and relates to the technical field of biological medicines and functional foods. The fermented dandelion composition with the ovarian cell repairing function is prepared by conducting composite enzymolysis and four-strain synergistic fermentation on whole dandelion leaf roots, enzyme preparations adopted in the composite enzymolysis are cellulase, pectinase and protease, and the weight adding ratio of the cellulase to the pectinase to the protease is 3: 2: 1; the strains adopted in the four-strain synergistic fermentation are aspergillus niger, saccharomycetes, lactic acid bacteria and bacillus subtilis, and the volume inoculation ratio is 2: 1: 1: 1. By innovating a four-bacterium fermentation process, the micromolecular protein content of the product reaches 12.8 g / 100g, which is 8.3 times that of the original medicinal materials, and is obviously higher than that of a single yeast fermentation product; meanwhile, dandelion polysaccharide is enriched, new-structure active substances such as AIP-1 and EPIG-1 are newly added, the oral bioavailability of rats reaches 68.9% and is far better than that of unfermented and single-strain fermented products, and the conversion and absorption efficiency of active ingredients is greatly improved.

The utility model relates to the technical field of reproductive auxiliary instruments, in particular to a reproductive clinical ovum taking device which comprises an ovum taking needle and a shell, one end of the shell is fixedly connected with a handle, one side of the handle close to the shell is hinged with a push handle, one side of the push handle close to the shell is hinged with a push rod, and the push rod penetrates through the shell and is in sliding fit with the shell. The end, away from the handle, of the shell is fixedly connected with a supporting rod, the end, away from the shell, of the supporting rod is fixedly connected with a fixing block, the fixing block is hinged to a rotating block, an arc-shaped sliding groove is formed in the fixing block, and a connecting rod is arranged in the sliding groove in a sliding fit mode. The egg taking needle is located between the fixed block and the rotating block, an egg storage cavity is formed in the shell, and the egg taking needle communicates with the egg storage cavity, penetrates through the shell and extends to the position between the fixed block and the rotating block; and an adsorption assembly for adsorbing egg cells is arranged on the egg storage cavity. The egg cell extractor is simple in structure, complete in function and capable of effectively assisting doctors to extract egg cells.

Provided is a Spodoptera frugiperda pupa ovary cell line with high Baculovirus production. The cell line has the name of IOZCAS-Sf-1 and the deposit number of CGMCC No. 21014. Further disclosed are a method for preparing the cell line and the use of the cell line in the large-scale growth of the Baculovirus. The cell line can be used for replicating the Baculovirus, for the large-scale production of Baculovirus insecticides and for constructing a Baculovirus expression vector system.