Galactomyces candidum and method for treating high-ammonia-nitrogen biogas slurry by using galactomyces candidum to produce single cell protein

A technology of Geotrichum candidum and biogas slurry, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of waste of resources, secondary pollution, etc., and achieve the effects of convenient operation, good growth, and excellent treatment effect

Active Publication Date: 2020-04-10
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a strain of Geotrichum candidum and a method for producing single-cell protein from biogas slurry with high ammonia nitrogen, aiming at the problems of secondary pollution and resource waste existing in the existing high ammonia nitrogen biogas slurry treatment technology

Method used

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  • Galactomyces candidum and method for treating high-ammonia-nitrogen biogas slurry by using galactomyces candidum to produce single cell protein
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  • Galactomyces candidum and method for treating high-ammonia-nitrogen biogas slurry by using galactomyces candidum to produce single cell protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment one: the screening of bacterial strain

[0047] 1. The present invention has tested dozens of strains that may be used in the treatment of high-ammonia nitrogen biogas slurry. In this embodiment, 14 representative strains are selected for display. The names, sources and culture conditions of each strain are as follows: Table 1 shows.

[0048] Table 1 shows the status of each strain

[0049]

[0050]

[0051]2. Display of the growth of each strain in chicken manure biogas slurry medium. The 14 kinds of bacteria in Table 1 were inoculated into the chicken manure biogas slurry medium with an ammonia nitrogen content of 1000mg / L (the ammonia nitrogen content was determined using Nessler's reagent photometry), using glucose to adjust C:N=10:1, using HCl Adjust the pH to 7.0, and set 3 parallels in each group. Under the same conditions and the same medium, no strain was used as the blank control group (CK). When cultured to 2 days and 4 days, the culture ...

Embodiment 2

[0067] Embodiment two: acquisition, purification and identification of Geotrichum candidum strain

[0068] Take distiller's grain samples from a distillery in Guiyang City, put them into a conical flask with 80 mL of sterile water, and culture them on a shaker at 160 rpm at 28 °C. After culturing for 12 hours, the concentration of 10 1 ~10 7 Gradient dilution, the selected concentration is 10 6 and 10 7 Dilutions were spread on solid yeast medium for more than three times. After culturing for 24 hours, pick a single colony with an inoculation loop, and then isolate and purify a single colony by streaking on a plate. Repeatedly pick a single colony and separate and purify it more than three times until the colony has the same shape, and the purified strain is obtained by microscopic examination, and finally transferred to the test tube on the slant of the solid yeast medium.

[0069] The isolated and purified strains were observed under a microscope, and the results were a...

Embodiment 3

[0071] Example 3: Effect of adding different carbon sources on fermentation

[0072] Select No. 5 Cha's culture medium as the selection medium, replace the carbon source (glucose) therein with xylose, sodium lactate, sodium acetate, sucrose containing xylose, sodium lactate, sodium acetate, sucrose (the same amount of carbon source specifically refers to equimolar quality C). The cell concentration was 1.25×10 9 2.5ml of CFU / mL Geotrichum candidum (ie 4% inoculum size) were respectively inoculated into the above-mentioned No. 5 Chapei medium containing different carbon sources, cultured at 30°C, and three parallels were set up for each treatment. It should be noted that the inoculation mentioned in the whole text refers to the inoculation of microorganisms grown to the logarithmic phase, and the % of the inoculum amount refers to the percentage of the volume of the bacteria to the volume of the inoculation substrate. Unless otherwise specified in the full text, the inoculati...

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Abstract

The invention belongs to the field of biological fermentation, and particularly relates to a galactomyces candidum and a method for treating high-ammonia-nitrogen biogas slurry by using the galactomyces candidum to produce single cell protein. The invention has the following specific technical scheme: the galactomyces candidum has a preservation number of CGMCC No.18570. The method of fermenting the biogas slurry comprises the following steps: taking the biogas slurry, performing filtration to remove large-particle impurities, adding a carbon source, adjusting a carbon-to-nitrogen ratio to 6:1to 10:1, and adjusting the pH to 5.5-7 to obtain a fermentation substrate; inoculating the fermentation substrate with the galactomyces candidum grown to a logarithmic stage, and performing fermentation at 20-30 DEG C; and after the fermentation is completed, performing centrifugation on the fermentation liquid, and drying the precipitate obtained after centrifugation at 50-60 DEG C to a constantweight to obtain the required single cell protein. The method only needs a period of biological reaction to treat the biogas slurry, has a low running cost and convenient operation, has an excellenttreatment effect on the biogas slurry, and can provide high economic value products, thereby providing an integrated and economic solution for biogas slurry treatment.

Description

technical field [0001] The invention belongs to the field of biological fermentation, and in particular relates to a Geotrichum candidum strain and a method for producing single-cell protein by treating high-ammonia-nitrogen biogas slurry with the same. Background technique [0002] In recent years, anaerobic digestion technology, also known as biogas fermentation technology, has been widely used to treat livestock manure, kitchen waste, and industrial organic wastewater. However, while producing clean energy (biogas or bionatural gas), a large amount of biogas slurry with high ammonia nitrogen content will be produced. It is a good choice to return biogas slurry to field as liquid fertilizer. However, due to the low fertilizer efficiency of biogas slurry and high transportation costs, it is difficult to return it to the field. In addition, when the application of biogas slurry exceeds the carrying capacity of the land, it is a kind of pollutant and will pollute the water ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14C12N1/145C12R2001/645
Inventor 李东周攀
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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