Method for extracting gibberellic acid GA4+7 from gibberellic acid fermentation liquor

A gibberellic acid and fermented liquid technology, applied in the direction of organic chemistry, can solve the problems of high production cost, low yield, and complexity

Inactive Publication Date: 2008-03-05
JIANGSU FENGYUAN BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Disadvantages of the British process: the purity of the separation is not high, and repeated extraction is required, which is relatively complicated. There are too many impurities in the fermentation filtrate of the Chinese process, and the content of the separated GA4+7 is not high, and a decolorization process is required. The above three patents have common shortcomings. The yield is low and the process is complicated
higher production costs

Method used

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  • Method for extracting gibberellic acid GA4+7 from gibberellic acid fermentation liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1): Get 20 tons of gibberellin A4+7 fermented liquid of gibberellin A4+7 bacterial classification through fermentation culture, measured pH value is 5.95, and fermentation unit is 872 μ / ml; Press out filtrate 16 through plate and frame filter press tons, the pH value is 5.90, and the appearance of the filtrate is light gray;

[0018] (2): above-mentioned filtrate is adjusted pH value with oxalic acid to be 4.0-5.0, then fully stirs 15 minutes, then enters plate and frame filter press again, utilizes original filter cake layer to filter, and filtrate after filtering is light yellow transparent body, this Press out 16.5 tons of filtrate through the plate and frame filter press;

[0019] (3): the former filter cake layer is washed with 10 tons of water with a pH value of 6.5-7.5, and the filtrate collected through cleaning is mixed with the filtrate obtained in the second step above;

[0020] (4) the mixed solution of the third step is regulated to 6.5-7.0 with soda ash ...

Embodiment 2

[0026] (1): Get 18.5 tons of gibberellin A4+7 fermented liquid of gibberellin A4+7 bacterial strain through fermentation culture, measured pH value is 6.12, and fermentation unit is 860 μ / ml; Press out filtrate 14.8 through plate and frame filter press tons, the pH value is 6.11, and the appearance of the filtrate is light gray;

[0027] (2): above-mentioned filtrate is adjusted pH value with oxalic acid to be 4.0-5.0, then fully stirs 15 minutes, then enters plate and frame filter press again, utilizes original filter cake layer to filter, and filtrate after filtering is light yellow transparent body, this Press out 15 tons of filtrate through the plate and frame filter press;

[0028] (3): the former filter cake layer is washed with 9 tons of water with a pH value of 6.5-7.5, and the filtrate collected through cleaning is mixed with the filtrate obtained in the second step above;

[0029] (4): the mixed solution of the third step is regulated to 6.5-7.0 with soda ash to 6.5...

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Abstract

The present invention is the technological process of extracting gibberellic acid GA4+7 from gibberellic acid fermenting liquid. The technological process includes the following steps: regulating the pH value of the filtrate of fermenting liquid to 4.5-4.8 before further filtering through available filter cake to eliminate protein, heavy metal and metal ion; regulating pH value to 6.5-6.8 before concentrating the filtrate by 18-20 times; regulating pH value to 4.2 before extracting with ethyl acetate; centrifuging in a high speed centrifuge, concentrating, diluting with n-butanol, precipitating in N.N-methyl benzylamine, and separating alcohol phase and water phase to obtain GA4+7 of 90 % over purity and GA4/GA7 ratio of 2 to 1. The present invention has high GA4+7 yield and low production cost.

Description

Technical field: [0001] The invention relates to a process for directly extracting GA4+7 from microbial fermentation broth. Background technique: [0002] At present, 82 kinds of gibberellic acid have been found in nature, which are arranged in order as GA1, GA2, GA3, GA4...GA82. These gibberellic acids mainly come from microorganisms and plants. The gibberellic acid species with the highest activity, such as GA3, GA4, GA7, etc., can all be found from microbial fermentation. Microbes also have the advantages of rapid growth, easy preparation, and high yield, so the current gibberellic acid products are all derived from microbial discovery. Almost all microorganisms use Gibberella vine as the producer. Most strains of this fungus produce a variety of gibberellic acids, such as GA3, GA4, GA7, GA9, etc. GA3 is the earliest gibberellic acid developed and applied. It has the strongest activity on plant stems, leaves, and growth promotion. GA4, GA7 has a relatively obvious elon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/93
Inventor 徐直
Owner JIANGSU FENGYUAN BIOENG
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