Plant growth regulator for promoting crop growth and preparation process thereof

Abstract

The invention is applicable to the technical field of planting industry, and provides a plant growth regulator for promoting crop growth and a preparation process thereof, the plant growth regulator comprises an agent A, an agent B and an agent C, the mass ratio of the agent A to the agent B to the agent C is (2-5): (2-3): (0.6-1.5); the agent A is prepared from a 14-hydroxyl brassinosterol soluble concentrate and a seaweed extract; the agent B comprises a porphyrin compound, turfy soil, humic acid, calcium carbonate, astragalus sinicus, propolis liquid and trace elements; the agent C comprises rhizobium, mycorrhizal fungi, a culture solution and saccharomycetes; preferably, the agent A is prepared from the following components in parts by mass: 0.4 to 1.1 parts of 14-hydroxybrassinosteroid soluble concentrate and 2 to 4 parts of seaweed extract. The product prepared by the invention not only can promote the growth of crops and improve the stress resistance and yield of the crops, but also can improve the soil structure and provide trace elements and organic matters required by the crops, and the preparation process is simple and easy to popularize and apply.

Description

Technical Field

[0001] The present invention relates to the technical field of planting industry, and specifically to a plant growth regulator for promoting crop growth and its preparation process. Background Art

[0002] The planting industry takes land as an important production material, utilizes green plants, synthesizes organic substances from carbon dioxide, water and minerals in nature through photosynthesis, and at the same time converts solar energy into chemical energy and stores it in organic substances. It is the material source of all foods based on plant products and is also the material basis for human life activities. The planting industry is an important foundation of large-scale agriculture. It is not only the main source of food and living materials for human survival, but also provides raw materials for the light textile industry and food industry, and provides feed for animal husbandry and fishery.

[0003] In the process of development, the planting industry often needs to use various growth regulators. For example: Chinese Patent with application number CN202210730178.6 and publication number CN114903057B discloses a plant regulator for improving the stress resistance of crops, its preparation method and application. The active ingredient of the plant regulator includes an extract of Amaranthus retroflexus, an Amaranthaceae plant. The raw material for obtaining the extract of Amaranthus retroflexus is preferably the root or stem of Amaranthus retroflexus. The plant regulator provided by this invention can significantly improve the tolerance of field crops under abiotic stress factors such as high temperature, low temperature, salinity and drought, can effectively improve the germination rate and seedling survival rate of field crops, and significantly reduce the yield loss under abiotic stress factors.

[0004] Chinese Patent with application number CN200910059591.9 and publication number CN101569310B provides a plant regulator for green fruits and vegetables. It consists of bioinformation substances, plant regulators and biological pesticides. It is mainly composed of chitosan, calcium chloride and abamectin. It is an ideal plant regulator for green fruits and vegetables. It will not cause harm to human health during its production and use, does not contain carcinogenic substances prohibited by environmental protection, and does not contain other polluting components. This fruit and vegetable plant regulator can enlarge the fruits of mangoes and increase the yield of mangoes. It can reduce the chemical pesticide residues on fruits without using chemical pesticides during the plant growth process. While increasing the yield of mangoes, it can also improve the fruit quality of mangoes and extend the sales time. It has the function of improving the fruit maturity and reducing the decay rate during the sales period. It achieves nutritional and health care effects. And it has the characteristics of simple method, low cost, strong operability and easy implementation. It can achieve good economic and social benefits.

[0005] The Chinese patent with the application number CN200410043747.1 and the publication number CN1293125C discloses a plant regulator of metallothionein and its processing method. By using bioengineering and nanotechnology, with plants and microorganisms as carriers, a regulator formula that can promote plant growth and stimulate the production of MT in plants is screened out from a large number of traditional Chinese medicine algae organic matters and minerals. It greatly improves the ability of plants to transform MT. It is the simplest, cheapest and safest high-tech biotechnology so far. The raw materials of the present invention: traditional Chinese medicine, trace elements, algae rich in alginic acid, enzyme bacteria, a mixture of nitrogen + phosphorus pentoxide + potassium oxide, and the rest is organic matter. Process method: 1. Mix the trace elements and then process them to 3-100 nanometer powder by nanotechnology; 2. Crush the traditional Chinese medicine substances to 80-120 mesh, mix them with the trace element powder and enzyme bacteria in corresponding proportions, place them in a fermentation tank, add negative ion water for fermentation, filter and remove the residue after fermentation, and take the fermentation broth for powder spraying and sterilization for standby; 3. Mix the algae organic matter containing 18-30% alginic acid and the mixture of nitrogen + phosphorus pentoxide + potassium oxide with the above fermentation powder evenly in a sterile room and then package it.

[0006] In summary, most of the existing plant growth regulators on the market have a single function, limited promotion effect on plant growth, complex preparation process and high cost, which limit their wide application in the planting industry. Therefore, it is particularly important to develop a plant growth regulator with diverse functions, simple preparation, low cost and effective promotion of crop growth. Summary of the Invention

[0007] The purpose of the present invention is to provide a plant growth regulator for promoting crop growth and its preparation process to solve the problems in the prior art that most plant growth regulators have a single function, limited promotion effect on plant growth, complex preparation process and high cost, which limit their wide application in the planting industry.

[0008] To achieve the above purpose, the present invention provides the following technical solutions: In the first aspect of the present invention, a plant growth regulator for promoting crop growth is provided, including agent A, agent B and agent C, and the mass ratio of agent A, agent B and agent C is (2-5):(2-3):(0.6-1.5); Agent A includes 14-hydroxy brassinolide soluble agent and seaweed extract; Agent B includes porphyrin compounds, peat soil, humic acid, calcium carbonate, milk vetch, propolis liquid, trace elements; Agent C includes rhizobia, mycorrhizal fungi, culture solution, yeast.

[0009] Preferably, by mass fraction, the content of each component of agent A is as follows: 14 - hydroxy brassinolide soluble agent 0.4 - 1.1 parts, seaweed extract 2 - 4 parts.

[0010] Preferably, by mass parts, the contents of the components of the Agent B are as follows: Porphyrin compound 3 - 7 parts, peat soil 2 - 5 parts, humic acid 1 - 7 parts, calcium carbonate 1.5 - 2.5 parts, milk vetch 0.3 - 1.1 parts, propolis liquid 1 - 4 parts, trace elements 0.3 - 0.8 parts.

[0011] Preferably, the contents of the components of the Agent C are as follows: Rhizobium (1 - 3)×10 10 CFU / ml, mycorrhizal fungi (0.4 - 1.2)×10 10 CFU / ml, culture medium 500 ml, yeast (1 - 2)×10 10 CFU / ml; The components and mass ratios in the culture medium are as follows: 1.5% - 2.5% sucrose, 0.08% - 0.12% potassium dihydrogen phosphate, 0.03% - 0.06% magnesium sulfate, 0.2% - 0.5% yeast extract, 0.01% - 0.03% sodium chloride, balance water.

[0012] Preferably, the mass fraction of 14 - hydroxy brassinolide in the 14 - hydroxy brassinolide soluble agent is 0.01%.

[0013] Preferably, the components and mass percentages of the 14 - hydroxy brassinolide soluble agent are as follows: 0.01% 14 - hydroxy brassinolide, 10% ethylene glycol, 0.1% sodium benzoate, 2.7% AEO - 9, 0.1% SAG1572, balance water.

[0014] Preferably, the porphyrin compound is one or more of magnesium protoporphyrin IX, heme or tetraphenylporphyrin.

[0015] Preferably, the trace elements include iron, zinc, potassium, phosphorus, calcium and magnesium.

[0016] Preferably, the mass ratio of iron, zinc, potassium, phosphorus, calcium and magnesium is (0.1 - 1):(0.5 - 0.8):(0.2 - 0.6):(0.5 - 1):(0.1 - 0.3):(0.2 - 0.5).

[0017] The second aspect of the present invention provides a preparation process of the plant growth regulator described in the first aspect of the present invention, including the following steps: S1, prepare Agent A: First, prepare the soluble solution of 14-hydroxy brassinolide as required. Then, at a temperature of 25-30 °C, mix the soluble solution of 14-hydroxy brassinolide and the seaweed extract, and stir to obtain Agent A; S2. Prepare Agent B: Mix and stir peat soil, milk vetch, and porphyrin compounds, and then add humic acid and propolis solution for mixing, and stir for 5-10 minutes to obtain Agent B; S3. Prepare Agent C: Prepare the culture solution, and then inoculate each strain according to the ratio to obtain Agent C.

[0018] The present invention has at least the following beneficial effects: A plant growth regulator for promoting crop growth and its preparation process provided by the present invention. The obtained product has a good plant growth regulation effect, and the preparation process is simple, the cost is low, and it is easy to promote and apply. Compared with the existing plant growth regulators on the market, the plant growth regulator provided by the present invention has more diverse functions. It can not only promote the growth of crops, improve the stress resistance and yield of crops, but also improve the soil structure, provide the trace elements and organic matter required by crops, and further promote the healthy growth of crops. Specific embodiments

[0019] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention. Example 1

[0020] This example provides a plant growth regulator for promoting crop growth, including Agent A, Agent B, and Agent C. The mass ratio of Agent A, Agent B, and Agent C is 2:2:0.6.

[0021] ①. Calculated by mass fraction, the components and contents of Agent A are as follows: 0.4 part of the soluble solution of 14-hydroxy brassinolide and 2 parts of the seaweed extract.

[0022] Among them, the components and mass percentages of the soluble solution of 14-hydroxy brassinolide are as follows: 0.01% of 14-hydroxy brassinolide, 10% of ethylene glycol, 0.1% of sodium benzoate, 2.7% of AEO-9 (fatty alcohol polyoxyethylene ether), 0.1% of SAG1572, and the balance is water.

[0023] ②. Calculated by mass fraction, the components and contents of Agent B are as follows: 3 parts of porphyrin compound, 2 parts of peat soil, 1 part of humic acid, 1.5 parts of calcium carbonate, 0.3 part of milk vetch, 1 part of propolis liquid, 0.3 part of trace elements.

[0024] Among them, the porphyrin compound is magnesium protoporphyrin IX.

[0025] Among them, the trace elements include iron, zinc, potassium, phosphorus, calcium and magnesium, and the mass ratio of iron, zinc, potassium, phosphorus, calcium and magnesium is 0.1:0.5:0.2:0.5:0.1:0.2.

[0026] ③. The content of each component of agent C is as follows: Rhizobium 1×10 10 CFU / ml, mycorrhizal fungi 0.4×10 10 CFU / ml, culture solution 500 ml, yeast 1×10 10 CFU / ml.

[0027] The components and mass ratio in the culture solution are: 1.5% sucrose, 0.08% potassium dihydrogen phosphate, 0.03% magnesium sulfate, 0.2% yeast extract, 0.01% sodium chloride, and the balance is water.

[0028] The preparation process of the plant growth regulator provided above includes the following steps: S1. Prepare agent A: First, prepare the soluble solution of 14-hydroxy brassinolide as required, and then at a temperature of 25°C, mix and stir the soluble solution of 14-hydroxy brassinolide and the seaweed extract to obtain agent A; S2. Prepare agent B: Mix and stir the peat soil, milk vetch, and porphyrin compound, and then add humic acid and propolis liquid for mixing and stirring for 5 minutes to obtain agent B; S3. Prepare agent C: Prepare the culture solution, and then inoculate each strain according to the ratio to obtain agent C.

[0029] Example 2 This example provides a plant growth regulator for promoting crop growth, including agent A, agent B and agent C, and the mass ratio of agent A, agent B and agent C is 3:2.5:1.

[0030] ①. By mass, the components and content of agent A are as follows: 1 part of soluble solution of 14-hydroxy brassinolide, 3 parts of seaweed extract.

[0031] Among them, the components and mass percentages of the soluble solution of 14-hydroxy brassinolide are as follows: 0.01% brassinolide, 10% ethylene glycol, 0.1% sodium benzoate, 2.7% AEO-9 (fatty alcohol polyoxyethylene ether), 0.1% SAG1572, balance water.

[0032] ②. By mass, the components and contents of agent B are as follows: 4 parts of porphyrin compound, 3 parts of peat soil, 6 parts of humic acid, 2 parts of calcium carbonate, 0.9 part of milk vetch, 2 parts of propolis liquid, 0.5 part of trace elements.

[0033] Among them, the porphyrin compound is heme.

[0034] Among them, the trace elements include iron, zinc, potassium, phosphorus, calcium and magnesium, and the mass ratio of iron, zinc, potassium, phosphorus, calcium and magnesium is 0.2:0.6:0.4:0.7:0.2:0.3.

[0035] ③. The content of each component of agent C is as follows: Rhizobium 2×10 10 CFU / ml, mycorrhizal fungi 1×10 10 CFU / ml, culture solution 500 ml, yeast 1.5×10 10 CFU / ml.

[0036] The components and mass ratio in the culture solution are: 2% sucrose, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.3% yeast extract, 0.02% sodium chloride, balance water.

[0037] The preparation process of the above-provided plant growth regulator includes the following steps: S1. Prepare agent A: First, prepare the brassinolide soluble agent as required, and then at a temperature of 27°C, mix and stir the brassinolide soluble agent and the seaweed extract to obtain agent A; S2. Prepare agent B: Mix and stir peat soil, milk vetch, and porphyrin compound, and then add humic acid and propolis liquid for mixing and stirring for 8 min to obtain agent B; S3. Prepare agent C: Prepare the culture solution, and then inoculate each strain in proportion to obtain agent C.

[0038] Example 3 This example provides a plant growth regulator for promoting crop growth, including agent A, agent B and agent C, and the mass ratio of agent A, agent B and agent C is 5:3:1.5.

[0039] ①. By mass, the components and contents of agent A are as follows: 1.1 parts of soluble 14 - hydroxy brassinolide and 4 parts of seaweed extract.

[0040] Among them, the components and mass percentages of the soluble 14 - hydroxy brassinolide are as follows: 0.01% of 14 - hydroxy brassinolide, 10% of ethylene glycol, 0.1% of sodium benzoate, 2.7% of AEO - 9 (aliphatic alcohol polyoxyethylene ether), 0.1% of SAG1572, and the balance is water.

[0041] ②. By mass, the components and contents of agent B are as follows: 7 parts of porphyrin compound, 5 parts of peat soil, 7 parts of humic acid, 2.5 parts of calcium carbonate, 1.1 parts of milk vetch, 4 parts of propolis liquid, and 0.8 part of trace elements.

[0042] Among them, the porphyrin compound is tetraphenylporphyrin.

[0043] Among them, the trace elements include iron, zinc, potassium, phosphorus, calcium, and magnesium, and the mass ratio of iron, zinc, potassium, phosphorus, calcium, and magnesium is 1:0.8:0.6:1:0.3:0.5.

[0044] ③. The contents of the components of agent C are as follows: Rhizobium 3×10 10 CFU / ml, mycorrhizal fungi 1.2×10 10 CFU / ml, culture medium 500 ml, yeast 2×10 10 CFU / ml.

[0045] The components and mass ratio in the culture medium are: 2.5% of sucrose, 0.12% of potassium dihydrogen phosphate, 0.06% of magnesium sulfate, 0.5% of yeast extract, 0.03% of sodium chloride, and the balance is water.

[0046] The preparation process of the above - provided plant growth regulator includes the following steps: S1. Prepare agent A: First, prepare the soluble 14 - hydroxy brassinolide according to requirements, and then at a temperature of 30°C, mix and stir the soluble 14 - hydroxy brassinolide and the seaweed extract to obtain agent A; S2. Prepare agent B: Mix and stir peat soil, milk vetch, and porphyrin compound, and then add humic acid and propolis liquid for mixing and stir for 10 min to obtain agent B; S3. Prepare agent C: Prepare the culture medium, and then inoculate each strain according to the ratio to obtain agent C.

[0047] As a plant hormone, the 14-hydroxy brassinolide soluble concentrate in Agent A of the present invention has the function of promoting plant cell division and growth, and can effectively improve the stress resistance and yield of crops; the seaweed extract is rich in various nutrients and growth factors required for plant growth, and can enhance the photosynthesis and nutrient absorption capacity of crops. The porphyrin compound in Agent B can enhance the photosynthesis efficiency of plants and improve the energy conversion ability of crops; components such as peat soil, humic acid and calcium carbonate can improve the soil structure and provide trace elements and organic matter required for crop growth; milk vetch and propolis liquid are rich in various bioactive substances, which can further promote the growth and development of crops. Rhizobia and mycorrhizal fungi in Agent C can form a symbiotic relationship with crops and promote the absorption and utilization of nutrients in the soil by crops; yeast can provide rich organic substances and microbial metabolites, and promote the ecological balance of the soil and the healthy growth of crops.

[0048] The relevant performance tests were carried out on the products prepared in the above Examples 1-3, and the results are as follows: Test on regulating the growth of citrus trees: Test crop: Citrus tree, variety: Satsuma mandarin.

[0049] Time and frequency of applying the growth regulator: At the early flowering stage of citrus, apply the growth regulator for the first time; at the young fruit stage of citrus, apply the growth regulator for the second time; at the fruit swelling stage of citrus, apply the growth regulator for the third time.

[0050] Application amount: The application amount per tree each time is 1200 ml - 1500 ml.

[0051] Set a blank control group (i.e., do not apply any medicament).

[0052] The results are shown in the following table: Test Items Blank Control Group Example 1 Example 2 Example 3 Number of Full-bloom Flowers (pcs) 56.84 60.31 61.05 60.56 Fruit-bearing Trees (pcs) 3.81 5.06 5.35 5.19 Fruit-setting Rate (%) 6.70 8.39 8.76 8.57 Single Fruit Weight of Fruit (g) 95.02 98.02 98.77 98.22 Yield (kg / tree) 25.11 27.35 27.64 27.40 Yield Increase Rate (%) / 8.92 10.07 9.12 Among them: The fruit setting rate and yield increase rate refer to "GB / T 17980.145-2004 Pesticide field efficacy test guidelines (part 2) - Part 145: Plant growth regulators for promoting flower formation and fruit setting in fruit trees":

[0053]

[0054] The number of full-bloom flowers was investigated during the full-bloom stage of citrus, and the single fruit weight of the fruit was the average weight of citrus fruits.

[0055] In summary, the product prepared by the present invention has a good yield increasing effect.

[0056] The foregoing has shown and described the basic principles, main features and advantages of the present invention. For those skilled in the art, it is obvious that the present invention is not limited to the details of the above-mentioned exemplary embodiments, and without departing from the spirit or basic features of the present invention, the present invention can be implemented in other specific forms. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, it is intended to embrace all changes that fall within the meaning and scope of the equivalent elements of the claims in the present invention.

[0057] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A plant growth regulator for promoting crop growth, characterized in that, It includes Agent A, Agent B and Agent C, and the mass ratio of Agent A, Agent B and Agent C is (2 - 5):(2 - 3):(0.6 - 1.5); Agent A includes 14 - hydroxy brassinolide soluble solution and seaweed extract; Agent B includes porphyrin compound, peat soil, humic acid, calcium carbonate, milk vetch, propolis liquid, trace elements; Agent C includes rhizobia, mycorrhizal fungi, culture solution, yeast; By mass, the contents of the components of Agent A are as follows: 0.4 - 1.1 parts of 14 - hydroxy brassinolide soluble solution and 2 - 4 parts of seaweed extract; By mass, the contents of the components of Agent B are as follows: 3 - 7 parts of porphyrin compound, 2 - 5 parts of peat soil, 1 - 7 parts of humic acid, 1.5 - 2.5 parts of calcium carbonate, 0.3 - 1.1 parts of milk vetch, 1 - 4 parts of propolis liquid, 0.3 - 0.8 parts of trace elements; The contents of the components of Agent C are as follows: Rhizobia (1 - 3)×10 10 CFU / ml, arbuscular mycorrhizal fungi (0.4 - 1.2)×10 10 CFU / ml, culture medium 500 ml, yeast (1 - 2)×10 10 CFU / ml; The components and mass ratio in the culture solution are as follows: 1.5% - 2.5% of sucrose, 0.08% - 0.12% of potassium dihydrogen phosphate, 0.03% - 0.06% of magnesium sulfate, 0.2% - 0.5% of yeast extract, 0.01% - 0.03% of sodium chloride, and the balance is water.

2. A plant growth regulator for promoting crop growth according to claim 1, characterized in that, The mass fraction of 14 - hydroxy brassinolide in the 14 - hydroxy brassinolide soluble solution is 0.01%.

3. The plant growth regulator for promoting crop growth according to claim 2, wherein The components and mass percentages of the 14 - hydroxy brassinolide soluble solution are as follows: 0.01% of 14 - hydroxy brassinolide, 10% of ethylene glycol, 0.1% of sodium benzoate, 2.7% of AEO - 9, 0.1% of SAG1572, and the balance is water.

4. A plant growth regulator for promoting crop growth according to claim 1, characterized in that, The porphyrin compound is one or more of magnesium protoporphyrin IX, heme or tetraphenylporphyrin.

5. A plant growth regulator for promoting crop growth according to claim 1, characterized in that, The trace elements include iron, zinc, potassium, phosphorus, calcium and magnesium.

6. A plant growth regulator for promoting crop growth according to claim 5, characterized in that, The mass ratio of iron, zinc, potassium, phosphorus, calcium and magnesium is (0.1 - 1):(0.5 - 0.8):(0.2 - 0.6):(0.5 - 1):(0.1 - 0.3):(0.2 - 0.5).

7. A preparation process of the plant growth regulator according to any one of claims 1 to 6, characterized in that, It includes the following steps: S1, preparing Agent A: First, prepare 14 - hydroxy brassinolide soluble solution as required, and then at a temperature of 25 - 30 °C, mix and stir the 14 - hydroxy brassinolide soluble solution and seaweed extract to obtain Agent A; S2, preparing Agent B: Mix and stir peat soil, milk vetch and porphyrin compound, and then add humic acid and propolis liquid for mixing and stirring for 5 - 10 min to obtain Agent B; S3, preparing Agent C: Prepare the culture solution, and then inoculate each strain according to the ratio to obtain Agent C.

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