119results about How to "For subsequent application" patented technology

The invention discloses controllable vector elimination method as well as an easy-to-use CRISPR-Cas9 tool. The invention provides a controllable vector elimination tool, which is composed of a control module and an elimination module, the control module including: a core zone which contains an elaborately-controlling promoter and a homing endonuclease encoding gene, two terminators respectively at two ends of the core zone, and homing endonuclease recognition sequences on the outer ends of the two terminators; and the elimination module including: an antibiotic resistance gene expression cassette, and homing endonuclease recognition sequences on the two ends; the tool can easily, quickly and high-effectively remove a plasmid vector in one step. A further developed easy-to-use CRISPR-Cas9 system can achieve one-step quick elimination to Cas9 and sgRNA encoding plasmids from a host cell. The easy-to-use CRISPR-Cas9 system is very convenient and practical, greatly saves experiment time, reduces experiment intensity, and is a very practical gene editing tool.

The invention provides a preparation method of graphene, which belongs to the technical field of synthesis of graphene and nano materials. The graphene is prepared by utilizing graphite oxide as a raw material and adopting environmental-friendly tea polyphenol / green tea juice as a reducing agent through a chemical reduction method without adding any stabilizing agent / dispersing agent. A graphene aqueous solution prepared by the invention has favorable dispersibility and stability without settling after standing for several months or generating an agglomerating phenomenon. The preparation method provided by the invention has advantages of simple process, easy popularization, low cost of the selected reducing agent, environmental protection and adaption of the requirements for low cost, large scale and green production. Any organic stabilizing agent / dispersing agent is not added in the process of preparing the graphene, which is beneficial to the following application of the graphene; and the adopted reducing agent and a reduzate which are environment-friendly have biocompatibility, and the prepared graphene is expected to be applied to the field of biochemistry.

The invention belongs to the field of the preparation of metal powder and relates to a method for preparing spherical porous silver powder. The spherical porous silver powder is prepared by a method combining gas atomization powder preparation and de-alloying. The method comprises the following steps of: firstly, smelting pure metal silver and magnesium according to a certain proportion; secondly, atomizing alloy melt with gas to prepare spherical alloy powder; and finally, performing de-alloying on micro-powder in salt solution and washing and drying the de-alloyed micro-powder. In the method, spherical porous micro-powder is obtained by combining spray powdering and de-alloying, selected etchant solution is non-toxic and harmless neutral salt solution, operation process is simple and process is environmentally-friendly and suitable for industrial mass production. By the method, components of the alloy and a metal phase removing process can also be designed according to requirements on the porosity and specific surface area of a final product, so the structure, size and hole pattern of the porous silver powder are controlled.

The invention discloses a gas-liquid pressurization cylinder. The gas-liquid pressurization cylinder is formed by the fact that a gas pressure cylinder body and a hydraulic cylinder body are in series connection through a middle cover with a middle hole and are separated into a pressurization gas cavity, a pre-pressing gas cavity, a pre-pressing oil storage cavity, a pressurization oil cavity and a stroke returning gas cavity through an upper end cover with a gas guide pipe, a pressurization piston with a hollow pressurization rod, a gas separation piston, the middle cover with the hole, a working piston and a lower end cover. The pressurization gas cavity, the pre-pressing gas cavity and the stroke returning gas cavity are respectively communicated with an exterior gas circuit through respective connectors, and the working processes of the pre-pressing stroke, the pressurization stroke and the reset stroke can be achieved through the control over the gas circuit. Compared with an existing gas-liquid pressurization cylinder, wherein the existing gas-liquid pressurization cylinder is provided with three or more cylinder bodies, and the cylinder bodies are in parallel connection or in serial connection, the gas-liquid pressurization cylinder has the advantages of being exquisite in structure, convenient to machine and high in use efficiency of a gas source. In addition, the design of the structure of conducting reasonable guiding and preventing rotation is added on an output working piston rod of the cylinder, and the installation and application of subsequent use are simplified.

The invention discloses a preparation method of pomegranate-shaped organic-inorganic nano-composite microspheres, which comprises the following steps: mixing an aqueous dispersion solution of silicon dioxide modified by proper amount of double-bond silane coupling agent in a coupling method with a phenylpropyl monomer, anionic emulsifier, non-anionic emulsifier and water, and stirring simply, so that a milky emulsified liquid is obtained; adding initiator to perform emulsion polymerization in the presence of silicon dioxide, so that the pomegranate-shaped organic-inorganic nano-composite microspheres with multiple silicon dioxide particles wrapped in phenylpropyl emulsion particles are obtained finally and are stably dispersed in the emulsified liquid. The preparation method has the advantages that the sources of the raw materials are extensive, the raw materials are easy to obtain, the preparation process is simple, and moreover, in the prepared pomegranate-shaped organic-inorganic nano-composite microspheres, the wrapping rate of silicon dioxide is high, the structure is neat and the placement stability is good. The adhesion property and the film forming performance of the composite microsphere particles are adjustable, the adhesive force and the transparency of the corresponding glue film are good, and the organic-inorganic nano-composite microspheres can be applied to the fields of coating, textile, rubber, plastics, biology, medicine and the like.

The invention provides a nanometer fibrils cellulose surface hydrophobic modification method. Alkenyl succinic anhydride or alkyl ketene dimer is grafted onto nanometer cellulose surface; partial hydrophile groups such as hydroxyl and carboxyl are replaced, so that the goal of changing the surface energy of the nanometer fiber is achieved, so that the super-hydrophilicity performance is convertedinto hydrophobic performance; finally, the application field can be expanded. The process has the advantages that the process operation is simple; the cost is low; the effect is obvious.

The invention belongs to the technical field of storage, regeneration and repair of cells and discloses low-temperature storage liquid for cells. The low-temperature storage liquid is mainly preparedfrom the following components in percent by mass: 0.8%-1.5% of mannitol, 2%-3% of human serum albumin, 3%-7% of low-molecular dextran, 0.1-0.3% of glutathione, 0.5%-2% of epigallocatechin gallate, 3%-5% of adenosine, 0.5%-1% of glucose, 0.8% of NaCl, 0.2% of KCl, 0.1% of CaCl2 and the balance of water. The invention also provides sodium alginate gel, which contains sodium alginate, calcium chloride, hyaluronic acid and the low-temperature storage liquid for the cells and the like, and also provides cell gel and a preparation method thereof as well as application of the low-temperature storageliquid for the cells, the sodium alginate gel and the cell gel. The invention has the advantages that the low-temperature storage liquid for the cells can maintain cell activity for longer time at temperature of 2-8 DEG C, the sodium alginate gel can be used as temporary short-term storage materials of cells from various sources and brackets of cell tissue engineering, and the cell gel can be usedas a medical wet wound dressing and can be used for repairing soft tissues, refractory-healing wound skins and mucosal wound injuries.

The invention discloses a disambiguation-free unsupervised part-of-speech tagging method based on error-correcting output codes. The disambiguation-free unsupervised part-of-speech tagging method comprises two main steps: (1) on the basis of a part-of-speech directory, generating training data; and (2) on the basis of the error-correcting output codes, carrying out training and testing. The disambiguation-free unsupervised part-of-speech tagging method does not need to tag corpora, can be applied to a part-of-speech tagging problem of a language which can not easily obtain the tagged corpus, and does not need to carry out disambiguation so as to avoid an error propagation problem in an iterative disambiguation process. A neuro-linguistic model is adopted to automatically generate characteristics used by training and testing so as to avoid the manual selection and construction of characteristics.

The invention relates to the technical field of energy and materials, in particular to a method and device for recycling metal from a waste lithium battery. The method comprises the following steps that the electrode material of the waste lithium battery is used as a cathode, and a noble electrode is used as an anode; and the cathode and the anode are placed in an electrolyte to carry out an electrochemical reaction, a mixed solution containing recycling metal ions is obtained, and the recycling metal ions comprise at least one kind of nickel ions, cobalt ions and manganese ions. The electrochemical reaction is utilized, and lithium, nickel, cobalt, manganese and other metal with high utilization value can be effectively recycled from a waste lithium battery electrode material.

The invention provides Annexin V-FITC exosome capture affinity magnetic beads, a preparation method thereof and an extraction method. The extraction method mainly comprises the following steps: (1) expression and purification of Annexin V, preparation of Annexin V-FITC, and construction of Annexin V-FITC exosome capture affinity magnetic beads; (2) cell culture and supernatant separation and concentration: separating out cell culture supernatant after the cell culture, and carrying out concentration treatment; (3) exosome extraction: co-incubating the cell culture supernatant obtained in the step (2) with the exosome capture affinity magnetic beads to obtain an affinity magnetic bead-exosome compound; and (4) exosome eluting: co-incubating the obtained affinity magnetic bead-exosome compound with an eluent, and collecting the eluent so as to obtain an extracted and separated exosome product. The exosome capture affinity magnetic beads disclosed by the invention can be repeatedly used and are convenient to extract, and the purity of the extracted exosome is high.

The invention provides an efficient preparation method of a boron nitride nanosheet and organic dispersion liquid thereof. The efficient preparation method comprises the following steps: a first step, adding boron nitride powder A, polymer and an organic solvent D in proportion, sealing, and then carrying out ultrasonic treatment to obtain initial dispersion liquid E, wherein the polymer is at least one of hyperbranched polyethylene B and hyperbranched polyethylene copolymer C; a second step, fully standing or centrifuging the initial dispersion liquid E obtained from the step 1 at low speed to obtain BNNSs dispersion liquid F containing excessive polymer; a third step, carrying out high-speed centrifuging or vacuum suction filtration and drying on the BNNSs dispersion liquid F containing the excessive polymer obtained from the step 2 to obtain a boron nitride nanosheet G; and a fourth step, adding the boron nitride nanosheet powder G and an organic solvent H which are obtained from the step 3 in the container, sealing and then carrying out ultrasonic treatment to obtain the boron nitride nanosheet dispersion liquid. By the efficient preparation method of the boron nitride nanosheet and the organic dispersion liquid thereof, the low-defect boron nitride nanosheet and the dispersion liquid thereof are effectively prepared controllably at low cost.

The invention discloses a method and system for preparing a winding-free high-efficiency amorphous ribbon. The method comprises the steps that a plurality of small-bore small melt spraying holes are formed in a melt nozzle at certain intervals, and after a mother alloy molten mass injected out of the small-bore small spraying holes is sprayed to a rapid quenching cooling roll face, the amorphous ribbon is formed independently; the system for preparing the winding-free high-efficiency amorphous ribbon comprises a blending device, a throw-preparing device and an amorphous ribbon automatic collector. The mother alloy is heated to be molten through the high-frequency induction technology, and the alloy melt is sprayed to the cooling roll face which is provided with a transverse groove and rotates fast through the spraying holes with the diameter smaller than 1 mm, wherein the surface of the cooling roll face is polished. The alloy melt sprayed to the cooled roll face is fast cooled on the smooth roll face to form the amorphous ribbon with even thickness and width.

The invention provides a stainless steel product surface passivation method and belongs to the technical field of stainless steel surfaces. According to the stainless steel product surface passivation method, a citric acid type passivation process replaces a strong acid and strong oxidant oriented passivation process. When the stainless steel product surface passivation method is utilized to perform stainless steel product surface passivation, complicated acid pickling or alkali washing activation process is not needed, only simple oil removing pretreatment is needed, and a stainless steel product with about 80 cm<2> passivation area can be passivated by using a 200 ml passivation solution.

The invention discloses shooting equipment and a shooting method. The shooting equipment comprises a supporting base, an optical lens, an image sensor, and a camera. The optical lens is arranged on the supporting base. The image sensor is arranged on the supporting base, and is used for converting an optical image entering the optical lens into an electric signal. The camera is arranged on the supporting base, and is coupled with the image sensor. The camera is used for shooting. The application performance of the shooting equipment is improved in order to facilitate the subsequent application.

The invention belongs to the technical field of preparation of nano materials, and particularly relates to multi-element doped graphene fibers and preparation and application thereof. Ionic liquid isused as a coagulation bath of graphene oxide, then uniformly loaded ionic liquid-graphene fibers are prepared by bonding active groups on the surface of graphene with ionic liquid, non-metal element co-doped graphene fibers are prepared through pyrolysis, and the ionic liquid serves as a nitrogen source, a boron source and a phosphorus source, so that the graphene fibers with good conductivity andhigh electrochemical activity are prepared. The multi-element doped graphene fibers can be used as a microelectrode in an electrochemical sensing system to detect small biological molecules. The problems that in the preparation method of the doped porous graphene fibers in the prior art, the quantity demand on a doping source is large, the process is complex, pollution is caused, the cost is high, and in actual detection, powder needs to coat an electrode are solved.

The invention provides bisphenol A polyether and a preparation method thereof. The preparation method of the bisphenol A polyether is simple in process; and a bisphenol A polyether product is obtained through reaction of bisphenol A and epoxyalkane, curing, degassing and antioxidant addition. By adopting a specific process, the uniform bisphenol A polyether product with low hydroxyl value, low viscosity and low chromaticity can be obtained. The bisphenol A polyether product is low in hydroxyl value, low in viscosity and low in chromaticity, can be kept uniform and stable for a long period of time, subsequent application can be better carried out, and mixing with other materials is facilitated.

The invention discloses a preparation method of nanometer fibrillated cellulose capable of being re-dispersed in water after drying, and belongs to the technical field of nanometer cellulose preparation. According to the present invention, nanometer fibrillated cellulose is physically modified with an anionic polymer electrolyte sodium carboxymethyl cellulose, and the obtained nanometer fibrillated cellulose and sodium carboxymethyl cellulose mixed solution is dried by an experimental fluidized bed granulator and a freeze drying machine to prepare powdery or microparticle-like nanometer fibrillated cellulose solid, wherein the nanometer fibrillated cellulose solid can be uniformly re-dispersed in an aqueous medium due to the weakening of the agglomeration caused by hydrogen bonding; and the preparation of the re-dispersible nanometer fibrillated cellulose provides convenience in storage, transportation and subsequent applications.

The invention provides a remote sensing image cloud detection method based on feature multi-scale perception and adaptive aggregation, and the method comprises the steps: a feature multi-scale perception module perceives context information of different scales through parallel expansion convolution pairs between an encoder and a decoder because the semantic information of clouds and cloud shadows on different scales is different; the feature adaptive aggregation module adaptively learns contributions of features of different scales to cloud and cloud shadow detection based on a self-attention mechanism, and realizes weighted aggregation of multi-scale features; the vector post-processing flow comprises operations such as hole filling, buffer area analysis, edge smoothing and erasing, so that the problems that a part of areas of a deep learning network detection result are fine and are zigzag are solved, an image effective area closer to manual production is finally obtained, and even the image effective area is more accurate than the image effective area obtained by manual production; a large amount of time and labor cost are saved for remote sensing image production, the finally obtained detection effect is good, and subsequent application of the image is facilitated.

The invention discloses a method for preparing a water-soluble polymer by auto-deposition polymerization initiated by ultraviolet light, belonging to the technical field of aqueous dispersion polymerization. The method comprises the following steps of: firstly, preparing reaction liquid: proportionally mixing a photoinitiator, a water-soluble monomer and a solvent in a straight tube glass reaction flask to prepare the reaction liquid, and introducing nitrogen for removing oxygen for 20-40 minutes, wherein the concentration of the photoinitiator in the reaction liquid is 0.1-10mmol / L, and the concentration of the monomer in the reaction liquid is 0.5-6mmol / L; and then, carrying out illumination reaction: putting the reaction flask containing the reaction liquid below an ultraviolet lamp for irradiating for 10-120 minutes. According to the invention, polymers formed in the reaction process are continuously precipitated out, gathered and deposited at the bottom of the reactor, so that the light shielding effect is relieved or eliminated; and meanwhile, the supernatant liquid can be recycled, so that the reaction efficiency is high, and the method is economic and rapid. Through simple decantation, a product can be separated, the method is simple and convenient, and the product is pure and can be dried easily, thereby being favorable for subsequent applications.

The invention relates to carbon nanotube conductive slurry and a preparation method and an application thereof. The preparation method comprises the steps of (1) mixing a surfactant and a solvent, andcarrying out preliminary pre-mixing dispersion to obtain a mixed solution; (2) adding nanotubes into the mixed solution obtained in the step (1) under a vacuum condition, and carrying out deep pre-mixing dispersion to obtain mixed slurry; and (3) carry out high-speed centrifugal dispersion on the mixed slurry obtained in the step (2) to obtain the carbon nanotube conductive slurry. Through the mutual cooperation of preliminary pre-mixing dispersion, deep pre-mixing dispersion and high-speed centrifugal dispersion, the dispersion effect of the carbon nanotubes is improved, and the viscosity ofthe conductive slurry is reduced, thereby improving the conductivity of the conductive slurry, and being conducive to the subsequent application of the conductive slurry. In addition, the conductiveslurry prepared according to the preparation method provided by the invention has the advantages of low viscosity, high solid content and excellent conductivity.

The present invention belongs to the technical fields of environmental microbiology and bioremediation, and specifically relates to an application of a bacillus PL-10 strain in low-temperature degradation of petroleum hydrocarbons. The use of the strain PL-10 with an ability to degrade the petroleum hydrocarbons at low temperatures is to degrade petroleum hydrocarbon pollutants in a microorganism-enhanced manner and for bioremediation of petroleum hydrocarbon-contaminated soil and water bodies; and the strain PL-10 and a corresponding nutrient solution thereof can effectively remove the petroleum hydrocarbon pollutants at 5-20 DEG C and have advantages of low cost, environmental friendliness, etc.

The invention provides a method and device for dividing a stratum of a target well, and relates to the technical field of stratum division. The method comprises the steps of determining a comprehensive well logging curve according to each well logging curve of the target well, specifically, the comprehensive well logging curve is used for synthesizing stratum property information corresponding tothe target well; carrying out similarity comparison on the comprehensive well logging curve and a preset comprehensive well logging curve of a reference well to respectively obtain a first similar well logging curve section of the comprehensive well logging curve and a second similar well logging curve section of the preset comprehensive well logging curve of the reference well; and according to astratum corresponding to the second similar well logging curve section, determining the stratum division condition in the first similar well logging curve section. According to the method and devicefor dividing the stratum of the target well, the stratum can be finely divided, and subsequent applications such as oil and gas reservoir development are facilitated.

The invention discloses a preparation method of a glycidyl methacrylate communicating porous material with high epoxy group content. The preparation method is characterized in that through high internal phase emulsion polymerization of glycidyl methacrylate (GMA) initiated by constant-temperature radiation, a communicating porous material of glycidyl methacrylate with high epoxy group content is obtained, and raw materials comprise glycidyl methacrylate, cross-linking agents, pore-foaming agents, emulsifying agents, water, inorganic salt and initiating agents. Compared with the reported glycidyl methacrylate communicating porous material, the glycidyl methacrylate communicating porous material prepared by using the method has the advantages of relatively high epoxy group content and relatively narrow cell apertures, and subsequent application of the glycidyl methacrylate communicating porous material is facilitated.

The invention provides a non-supervision and Fourier kernel function based remote sensing image pixel purity identification method. The non-supervision and Fourier kernel function based remote sensing image pixel purity identification method comprises performing segmentation on the original remote sensing image to obtain P objects; performing clustering operation on the P objects to obtain n block masses; setting any block mass ti to comprise d objects and executing following operation which comprises calculating the Fourier kernel function hull convexity which is corresponding to the block mass ti, calculating the distance from the specified object Vf to every object in the b objects to obtain the object T which has the smallest distance from the specified object Vf, wherein the distance from the specified object Vf to the object T is M1, calculating the distance M2 from the specified object Vf to the center of the hull convexity, calculating the distance M3 from the object T to the center of the hull convexity, and calculating the mapping distance value M2 / (M3+M1) which serves as the integral purity value of the specified object Vf. The non-supervision and Fourier kernel function based remote sensing image pixel purity identification method serves as a quantitative method and accordingly the pixel purity of a remote sensing image can be identified accurately.

The invention provides a preparation method of high-dispersibility soybean protein concentrate. The preparation method of the high-dispersibility soybean protein concentrate comprises the following steps: mixing low-temperature skimmed soybean meal with alkali water of which the pH value is 9.0-12.0, performing high-speed continuous shearing, adjusting the pH value to 4.0-5.0 so as to be subjectedto acid precipitation, and performing solid-liquid separation so as to obtain a mixture of soybean proteins and bean dregs; adjusting the pH value to 7.4-8.0 and the sugar content to 8.0-11.0; and then, carrying out sterilization, flash distillation, high-pressure homogenization and spraying-drying so as to obtain the high-dispersibility soybean protein concentrate, wherein coating liquid used for the spraying-drying process is prepared from phospholipids, sucrose fatty acid ester and polydimethylsiloxane as main raw materials. The preparation method of the high-dispersibility soybean proteinconcentrate is simple in production processes and capable of ensuring low soybean protein concentrate degradation degree. The prepared soybean protein concentrate preserves original flavor and functional characteristics, and has the characteristics of high dispersibility. The soybean protein concentrate is relatively good in dispersibility and solubility, so that the soybean protein concentrate can quickly dissolve in water; moreover, the soybean protein concentrate is not liable to cake, so that the soybean protein concentrate is beneficial for application in the fields of milk products, nutritious formula products, meat products, health products and the like.

The invention provides a preparation method of graphene, which belongs to the technical field of synthesis of graphene and nano materials. The graphene is prepared by utilizing graphite oxide as a raw material and adopting environmental-friendly tea polyphenol / green tea juice as a reducing agent through a chemical reduction method without adding any stabilizing agent / dispersing agent. A graphene aqueous solution prepared by the invention has favorable dispersibility and stability without settling after standing for several months or generating an agglomerating phenomenon. The preparation method provided by the invention has advantages of simple process, easy popularization, low cost of the selected reducing agent, environmental protection and adaption of the requirements for low cost, large scale and green production. Any organic stabilizing agent / dispersing agent is not added in the process of preparing the graphene, which is beneficial to the following application of the graphene; and the adopted reducing agent and a reduzate which are environment-friendly have biocompatibility, and the prepared graphene is expected to be applied to the field of biochemistry.

The invention discloses a nitrogen elastic pressing type composite edge cutting device and a production process thereof. The nitrogen elastic pressing type composite edge cutting device comprises a sample clamping system, a lofting system, a sample moving system, an opening system, a cooling system, fixed cutting systems, a movable cutting system, a cleaning system and a polishing system. According to the nitrogen elastic pressing type composite edge cutting device, by adopting manual intervention, on one hand, the probability of production accidents is greatly reduced, and on the other hand,the sample injection and material moving processes are standardized and unified, and the subsequent processing process can be subjected to quality control. According to the nitrogen elastic pressing type composite edge cutting device, a ceramic material plate is opened by the opening system before cutting and trimming, and an opening is formed in the surface of the ceramic material plate, so thatnot only is a large amount of loss of cutting lines and the like caused by factors such as surface stress in the subsequent cutting process avoided, but also the material plate is prevented from beingdamaged by a large amount of extra heat generated in the machining process due to abraded cutting lines.

The invention provides 4,4-diaminodiphenyl methane polyether and a preparation method thereof. The preparation method of 4,4-diaminodiphenyl methane polyether is simple in process and spends a short time. The preparation method comprises the steps of enabling 4,4-diaminodiphenyl methane to react with alkyleneoxide, adding an antioxidant, and carrying out ripening, degassing and neutralization, so as to obtain the 4,4-diaminodiphenyl methane polyether product. The 4,4-diaminodiphenyl methane polyether product synthesized by virtue of a specific process is low in hydroxyl value, viscosity and chromaticity and high in reaction activity, does not contain water, can remain uniform and stable for a long time, can be well applied to subsequent use and can be well mixed with other materials.

The invention discloses a dynamic updating method for three-dimensional live-action data of a large scene. The method comprises the following steps of sequentially performing coordinate conversion framing and spatial coding on the effective three-dimensional live-action data to be input and updated, calculating an effective range, and performing data fusion updating according to spatial coding andthe effective range to realize dynamic updating of the three-dimensional live-action data; according to the invention, full automation of the dynamic updating process is truly realized, the risk of errors caused by manual operation is reduced, the cost of data management is reduced, and the invention is suitable for dynamic updating of three-dimensional live-action data of a large scene; standardized processing of various three-dimensional live-action data is achieved, and the method can adapt to various types of three-dimensional live-action data produced by different software and differententerprises and has extremely high adaptability. More importantly, organic fusion of the historical three-dimensional live-action data and the effective three-dimensional live-action data to be inputand updated is realized, and high quality and high precision of the data are ensured.

The present disclosure provides a process for synthesizing 2, 2, 4, 4-tetramethyl-1, 3-cyclobutanedione, comprising the steps of (a) cleaving isobutyric anhydride to form a dimethyl ketene-containinggas; (b) introducing gas containing dimethyl ketene into an ester solvent to dissolve the dimethyl ketene in the ester solvent to form an ester solution containing the dimethyl ketene; and (c) standing the ester solution containing the dimethyl ketene under inert gas, so that the dimethyl ketene is dimerized to form the 2, 2, 4, 4-tetramethyl-1, 3-cyclobutanedione. The synthetic method of the present invention is a synthetic method of collecting DMK generated by cracking IBAN in a new manner and dimerizing DMK to form TMCD, thereby being capable of basically overcoming various defects in the prior art.