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Application of bacillus in low-temperature degradation of petroleum hydrocarbons

A technology of Bacillus and Bacillus subtilis, which is applied in the field of bioremediation and environmental microbiology, can solve the problems of insufficient resources of low-temperature degrading bacteria strains, and is beneficial to industrial production and subsequent applications, with strong growth and colonization capabilities, and growth good reproductive effect

Active Publication Date: 2020-10-02
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a kind of bacillus PL-10 in the present situation that low-temperature degrading bacteria strain resources are insufficient in the current petroleum hydrocarbon pollution biological treatment technology Applications in Low Temperature Degradation of Petroleum Hydrocarbons

Method used

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  • Application of bacillus in low-temperature degradation of petroleum hydrocarbons
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  • Application of bacillus in low-temperature degradation of petroleum hydrocarbons

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Screening of strain PL-10

[0049] Take 15g of oily sludge, put it into a 250mL Erlenmeyer flask pre-installed with 100mL of seed liquid medium, and then shake it on a constant temperature shaker at 30°C and 180rpm for 72h;

[0050] After 72 hours, the supernatant bacterial solution after standing still was taken and spread on the screening medium plate after 10-fold serial dilution. The content of crude oil in the screening medium is 1.0g / L;

[0051] The composition of seed liquid culture medium is: glucose 9g, (KH 4 ) 2 SO 4 10.0g, KCl 1.1g, NaCl 1.1g, KH 2 PO 4 3.4g, K 2 HPO 4 4.4g, MgSO 4 0.5g, 0.4g of yeast extract, 0.5mL of trace element solution, 1.5mL of vitamin solution, 1000mL of distilled water, and adjust the pH to 7.

[0052] The composition of the inorganic salt medium is: (KH 4 ) 2 SO 4 9.0g, KCl 1.0g, NaCl 1.2g, KH 2 PO 4 3.3g, K 2 HPO 4 4.0g, MgSO 4 0.2g, 0.5g of yeast extract, 0.5mL of trace element solution, 1.5mL of vitamin...

Embodiment 2

[0061] The PL-10 strain obtained above was inoculated into a 250mL Erlenmeyer flask containing 100mL seed liquid medium, and cultured on a constant temperature shaker at 30°C and 180rpm for 48h. After 48 hours, take the PL-10 bacterial liquid, inoculate it into the screening medium according to the inoculation ratio of 2%, and then culture it at 20°C, 10°C, and 5°C for 150 days, and transfer it to the newly prepared screening medium every 50 days Middle; Repeat the constant temperature culture process for 3 rounds to obtain the low-temperature acclimated seed solution of the strain, which will be used later.

[0062] Test of Degradation of Petroleum Hydrocarbon Pollutants in Soil Using PL-10 at 20°C

[0063] Inoculate the acclimatized bacterial solution obtained from the above 20°C culture into the petroleum hydrocarbon-contaminated soil according to the inoculation ratio of 5%; another treatment without inoculation of the PL-10 bacterial solution is set. The water content of...

Embodiment 3

[0067] Test of Degradation of Petroleum Hydrocarbon Pollutants in Soil Using PL-10 at 10℃

[0068] Inoculate the acclimatized bacterial solution obtained from the above 10°C culture into the petroleum hydrocarbon-contaminated soil according to the inoculation ratio of 5%; another treatment without inoculation of the PL-10 bacterial solution is set. The water content of the mixed sample was adjusted to 30% with inorganic salt medium, and the pH was adjusted to 7.5. After sealing with an air-permeable sealing film, the plastic beaker was placed in a constant temperature incubator at 10°C for static cultivation. Sampling every 30 days adopts Soxhlet extraction method-gravimetric method to measure the total petroleum hydrocarbon content in the oil sludge (see Figure 4 ). Among them, petroleum hydrocarbons pollute the soil, and its initial oil content is 2.0%.

[0069] Depend on Figure 4 It can be seen from the recorded measurement results that the total petroleum hydrocarbon...

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Abstract

The present invention belongs to the technical fields of environmental microbiology and bioremediation, and specifically relates to an application of a bacillus PL-10 strain in low-temperature degradation of petroleum hydrocarbons. The use of the strain PL-10 with an ability to degrade the petroleum hydrocarbons at low temperatures is to degrade petroleum hydrocarbon pollutants in a microorganism-enhanced manner and for bioremediation of petroleum hydrocarbon-contaminated soil and water bodies; and the strain PL-10 and a corresponding nutrient solution thereof can effectively remove the petroleum hydrocarbon pollutants at 5-20 DEG C and have advantages of low cost, environmental friendliness, etc.

Description

technical field [0001] The invention belongs to the technical fields of environmental microbiology and bioremediation, and in particular relates to the application of a strain of bacillus PL-10 in low-temperature degradation of petroleum hydrocarbons. Background technique [0002] Petroleum is a very important energy and chemical raw material. With the rapid development of industry in human society, the global consumption of oil is increasing day by day. There is a certain risk of crude oil leakage in the process of oil field exploration, oil extraction, and oil transportation. Incidents related to oil spills and pollution occur frequently around the world, causing oil to enter the soil and water environment, causing serious pollution. Petroleum is mainly composed of about 200 kinds of hydrocarbon compounds, which contain a variety of carcinogenic and teratogenic compound components, which pose a serious threat to the quality and safety of the ecological environment. [0...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/125
CPCC12N1/20B09C1/10C12R2001/125C12N1/205
Inventor 张颖雷丽莹韩斯琴史荣久
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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