Application of bacillus in low-temperature degradation of petroleum hydrocarbons

A technology of Bacillus and Bacillus subtilis, which is applied in the field of bioremediation and environmental microbiology, can solve the problems of insufficient resources of low-temperature degrading bacteria strains, and is beneficial to industrial production and subsequent applications, with strong growth and colonization capabilities, and growth good reproductive effect

Active Publication Date: 2020-10-02
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a kind of bacillus PL-10 in the present situation that low-temperature degrading bacteria strain resources

Method used

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  • Application of bacillus in low-temperature degradation of petroleum hydrocarbons
  • Application of bacillus in low-temperature degradation of petroleum hydrocarbons
  • Application of bacillus in low-temperature degradation of petroleum hydrocarbons

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0047] Example 1

[0048] (1) Screening of strain PL-10

[0049] Take 15g of oily sludge, put it into a 250mL Erlenmeyer flask pre-filled with 100mL seed liquid culture medium, and then shake and cultivate for 72h on a constant temperature shaker at 30°C and 180rpm;

[0050] After 72h, take the standing upper bacterial solution and spread it on the screening medium plate after 10-fold dilution. The crude oil content in the screening medium is 1.0g / L;

[0051] The composition of the seed liquid medium is: glucose 9g, (KH 4 ) 2 SO 4 10.0g, KCl 1.1g, NaCl 1.1g, KH 2 PO 4 3.4g, K 2 HPO 4 4.4g, MgSO 4 0.5g, yeast extract 0.4g, trace element solution 0.5mL, vitamin solution 1.5mL, distilled water 1000mL, adjust the pH to 7.

[0052] The composition of the inorganic salt medium is: (KH 4 ) 2 SO 4 9.0g, KCl 1.0g, NaCl 1.2g, KH 2 PO 4 3.3g, K 2 HPO 4 4.0g, MgSO 4 0.2g, yeast extract 0.5g, trace element solution 0.5mL, vitamin solution 1.5mL, distilled water 1000mL, adjust the pH to 7.

[0...

Example Embodiment

[0060] Example 2

[0061] Take the above-obtained PL-10 strain, inoculate it into a 250 mL conical flask containing 100 mL of seed liquid culture medium, and culture it on a constant temperature shaker at 30° C. and 180 rpm for 48 hours. After 48h, take the PL-10 bacterial solution, inoculate it into the screening medium according to the inoculation ratio of 2%, and then incubate at 20℃, 10℃, 5℃ for 150 days, and transfer to the newly prepared screening medium every 50 days Medium; Repeat 3 rounds of constant temperature culture process to obtain low-temperature acclimation seed liquid of strains for use.

[0062] Test of using PL-10 to degrade petroleum hydrocarbon pollutants in soil at 20℃

[0063] The domesticated bacterial solution obtained by the above-mentioned 20°C culture was inoculated into petroleum hydrocarbon contaminated soil at a 5% inoculation ratio; another treatment was not to inoculate the PL-10 bacterial solution. Adjust the water content of the mixed sample to 3...

Example Embodiment

[0066] Example 3

[0067] Test of using PL-10 to degrade petroleum hydrocarbon pollutants in soil at 10℃

[0068] The domesticated bacteria liquid obtained by the above-mentioned 10°C cultivation is inoculated into petroleum hydrocarbon contaminated soil according to the inoculation ratio of 5%; another treatment is not to inoculate the PL-10 bacterial liquid. Adjust the water content of the mixed sample to 30% with an inorganic salt medium, and adjust the pH to 7.5. After sealing with a gas-permeable sealing film, place the plastic beaker in a constant temperature incubator at 10°C and let it stand for constant temperature culture. Sampling every 30d, the Soxhlet extraction method-gravimetric method is used to determine the total petroleum hydrocarbon content in the sludge (see Figure 4 ). Among them, petroleum hydrocarbons pollute the soil, and its initial oil content is 2.0%.

[0069] by Figure 4 The recorded measurement results show that the total petroleum hydrocarbon degra...

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Abstract

The present invention belongs to the technical fields of environmental microbiology and bioremediation, and specifically relates to an application of a bacillus PL-10 strain in low-temperature degradation of petroleum hydrocarbons. The use of the strain PL-10 with an ability to degrade the petroleum hydrocarbons at low temperatures is to degrade petroleum hydrocarbon pollutants in a microorganism-enhanced manner and for bioremediation of petroleum hydrocarbon-contaminated soil and water bodies; and the strain PL-10 and a corresponding nutrient solution thereof can effectively remove the petroleum hydrocarbon pollutants at 5-20 DEG C and have advantages of low cost, environmental friendliness, etc.

Description

technical field [0001] The invention belongs to the technical fields of environmental microbiology and bioremediation, and in particular relates to the application of a strain of bacillus PL-10 in low-temperature degradation of petroleum hydrocarbons. Background technique [0002] Petroleum is a very important energy and chemical raw material. With the rapid development of industry in human society, the global consumption of oil is increasing day by day. There is a certain risk of crude oil leakage in the process of oil field exploration, oil extraction, and oil transportation. Incidents related to oil spills and pollution occur frequently around the world, causing oil to enter the soil and water environment, causing serious pollution. Petroleum is mainly composed of about 200 kinds of hydrocarbon compounds, which contain a variety of carcinogenic and teratogenic compound components, which pose a serious threat to the quality and safety of the ecological environment. [0...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/125
CPCC12N1/20B09C1/10C12R2001/125C12N1/205
Inventor 张颖雷丽莹韩斯琴史荣久
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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