Bioremediation method for petroleum-polluted soil

A technology of oil pollution and bioremediation, applied in the field of bioremediation of oil-contaminated soil, can solve the problems of low degradation efficiency, long duration, and low cost, and achieve good repair effect, short repair time and simple method

Active Publication Date: 2015-12-23
李磊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the characteristics of low cost, good effect, and no secondary pollution, but it also has certain limitations. The survival rate and survival time of the functional microorganisms put in are greatly affected by the changing environmental physical and chemical factors, which directly determine The utilization rate of the functional microorganisms and the degradation rate of pollutants
[0005] At present, when microorganisms are used to remediate oil-contaminated soil, it is often only to add some kind of active bacteria or a mixed bacterial solution at the beginning of the treatment, and to use a single control condition from the beginning to the end, and no adj

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Bioremediation of oil-based mud with oil content up to 30 wt%

[0029] The first repair stage: firstly, add the above-mentioned biomass carrying biomass-degrading bacteria to the oil-based mud to be treated and mix it. The percentage of the added amount of biomass carrying biomass-degrading bacteria in the oil-based mud to be treated is 30wt%, while keeping the water content of the oil-based mud at 30wt%, the first degrading bacteria were added on the 12th day after plowing, wherein the dosage of bacteria was 10 10 CFU / m 2 Around the soil, the dosage of fungi is 35mg dry weight / m 2 Soil, bacteria mixture of P. aeruginosa and Enterobacter cloacae, fungus mixture of Phanerochaetechrysosporium and Cunninghamellaechinulata; tillage mix weekly 2 times, three weeks later, the oil content in the mud was measured to be about 10%.

[0030] The second repair stage: firstly add potassium nitrate and potassium hydrogen phosphate to the mud after the first repair stage,...

Embodiment 2

[0034] Example 2 Bioremediation of oil-based mud with oil content up to 30 wt%

[0035] The first repair stage: firstly, add the above-mentioned biomass carrying biomass-degrading bacteria to the oil-based mud to be treated and mix it. The percentage of the added amount of biomass carrying biomass-degrading bacteria in the oil-based mud to be treated is 25wt%, while keeping the water content of the oil-based mud at 30wt%, the first degrading bacteria were added on the 10th day after plowing, wherein the dosage of bacteria was 10 10 CFU / m 2 Around the soil, the dosage of fungi is 35mg dry weight / m 2 Soil, the bacteria is Pseudomonas aeruginosa (P.Aeruginosa), the fungus is a mixture of Phanerochaetechrysosporium and Cunninghamellaechinulata; plow and mix once a week, after three weeks The oil content in the mud was determined to be about 15%.

[0036] The second repair stage: firstly add potassium nitrate and potassium hydrogen phosphate to the mud after the first repair sta...

Embodiment 3

[0039] Example 3 Bioremediation of oil-based mud with oil content up to 30 wt%

[0040] The first repair stage: firstly, add the above-mentioned biomass carrying biomass-degrading bacteria to the oil-based mud to be treated and mix it. The percentage of the added amount of biomass carrying biomass-degrading bacteria in the oil-based mud to be treated is 30wt%, while keeping the water content of the oil-based mud at 30wt%, the first degrading bacteria were added on the 12th day after plowing, wherein the dosage of bacteria was 10 10 CFU / m 2 Around the soil, the dosage of fungi is 35mg dry weight / m 2 In the soil, the bacterium is Micrococcus luteus, and the fungus is Phanerochaetechrysosporium (Phanerochaetechrysosporium). Plow and mix twice a week, and the oil content in the mud is measured to be about 18% after three weeks.

[0041] The second repair stage: firstly add potassium nitrate and potassium hydrogen phosphate to the mud after the first repair stage, so that the mol...

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Abstract

The invention discloses a bioremediation method for petroleum-polluted soil. The method comprises the steps that 1, biomass containing biomass degrading bacteria is added in the petroleum-polluted soil with the petroleum content higher than 18 wt%, and ploughing is performed to mix the soil with the biomass, the water content of the petroleum-polluted soil is simultaneously kept at 10-30 wt%, and first degrading bacteria are added in the soil in 10-15 days after ploughing is performed to reduce the petroleum content of the soil to 8-18 wt%; 2, second degrading bacteria are added in the soil to reduce the petroleum content of the soil to 5-8 wt%; 3, third degrading bacteria are added in the soil to reduce the petroleum content of the soil to 1-5 wt%; 4, forth degrading bacteria are added in the soil to reduce the petroleum content of the soil below 1 wt%. The method can be applied to both in-situ remediation and ex-situ remediation, the remediation effect is good, the remediation time is short, the method is simple, and the cost is low.

Description

technical field [0001] The invention relates to a restoration method of polluted soil, in particular to a bioremediation method of petroleum-contaminated soil. Background technique [0002] With the prosperity and development of our country's social economy, people's demand for oil is increasing, thus promoting the development of oil exploration. However, due to technical and management defects, a large amount of crude oil directly or indirectly flows into the soil, thereby polluting the soil, so that the oil fills the gaps in the soil at a certain depth, affects the permeability of the soil, and destroys the soil water, gas and solids of crude oil. The three-phase structure affects the growth of microorganisms in the soil, and also affects the respiration of plant roots in the soil and the absorption of water and nutrients, and even causes the root system of plants to rot and necrosis, seriously endangering the growth of plants. And the oil in the soil runs along with the ...

Claims

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Application Information

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IPC IPC(8): B09C1/10
CPCB09C1/105
Inventor 李磊
Owner 李磊
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