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Improved adipose-derived stem cells for cartilage injury repair

A technology for fat stem cells and cartilage damage, applied to cells modified by introducing foreign genetic material, plant genetic improvement, and methods based on microorganisms. It can solve the problems of short half-life and inconvenient clinical treatment, and achieve the effect of promoting growth and prolonging repair Effect

Inactive Publication Date: 2013-05-08
GUANGZHOU LAND BIOLOGY TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these growth factors can be used by injection, their half-life is very short, requiring a large amount of one-time injection or repeated injections to achieve the desired concentration, which causes inconvenience to clinical treatment

Method used

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  • Improved adipose-derived stem cells for cartilage injury repair
  • Improved adipose-derived stem cells for cartilage injury repair
  • Improved adipose-derived stem cells for cartilage injury repair

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Embodiment Construction

[0018] In order to describe the technical content of the present invention, the objectives and effects achieved, the following will be described in detail in conjunction with the embodiments and accompanying drawings. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. In addition, it should be understood that after reading the authorization of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

[0019] 1. Isolation and culture of human adipose-derived stem cells

[0020] 1) About 10 days before cartilage sampling, about 20ml of fat was extracted from the patient's thigh or abdomen, and the cells of the fat interstitial blood vessels were separated and cultured and passaged: the fat tissue was taken under ...

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Abstract

The adipose-derived stem cells are derived to express bone morphogenetic protein (BMP-4) or transforming growth factor (TCF-beta3) through a gene transduction technology. The performance-enhanced stem cell has high multiplication capacity and cartilage repair capacity and is a good tool for repairing cartilage injury. Considering the functions of the BMP-4 and TCF-beta3 on stem cell regulation and nutrition, the trophic factors are fully expressed by the stem cells through a virus vector transduction method, the capacity of culturing the improved adipose-derived stem cells and cartilage cells in a cartilage induction medium for differentiating into cartilage cells is higher than that of the common adipose-derived stem cells, more type II collagens are generated, and the formed cartilage sphere is large and dense; and meanwhile, the survival cycle of the transplanted stem cells in vivo is prolonged, the repair function of the growth factors and trophic factors on the damaged part is prolonged, and cartilage growth and wound healing are promoted.

Description

technical field [0001] The invention relates to modifying stem cells by genetic technology to improve their performance and the expression of secreted proteins, and the modified cells can be used in the repair of cartilage damage. Background technique [0002] Cartilage damage is a difficult disease to heal, especially superficial cartilage damage. Cartilage damage can be caused by a variety of reasons, such as external impact, autoimmune diseases such as rheumatoid arthritis, and body degeneration caused by aging. The self-repair ability of cartilage is very weak, even a small cartilage defect is difficult to heal. Because there is no blood supply in the cartilage tissue, the synthesis activity of the endochondral matrix is ​​low. But if the wound goes deep to the subchondral bone layer, the wound has the ability to heal itself. This is because the blood in the subchondral bone seeps out to form a blood clot with stem cells attached, which may be activated to promote woun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/867A61L27/38C12R1/91
Inventor 付擎昊康贤通章戴荣
Owner GUANGZHOU LAND BIOLOGY TECH
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