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Preparation method of sturgeon bone II-type collagen peptide

A collagen peptide and sturgeon technology, applied in chemical instruments and methods, specific peptides, connective tissue peptides, etc., can solve problems such as poor uniformity and achieve good joint health care functions.

Pending Publication Date: 2017-11-24
HAILISHENG GROUP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the collagen peptides extracted by the existing collagen peptide extraction and preparation methods have a wide molecular weight distribution and poor uniformity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Choose fresh sturgeon cartilage and sturgeon skull as raw materials for collagen extraction, wash them, chop them up, add 8 times the volume (w / v) of 0.05mol / L NaOH solution and stir for 2 hours, rinse with running water until neutral, and mix well. Add 4 times the volume of purified water to the slurry, treat at 110°C for 40 minutes, cool down to 75°C for 3 hours, centrifuge and filter at 4000 rpm, add 2 times the volume of water to the residue and continue to extract at 75°C for 1 hour, centrifuge at 4000rpm to remove the residue, and combine The two extracts are the sturgeon bone type II collagen extract; adjust the concentration of collagen in the concentrated solution to 4%, add trypsin to it, the addition amount is 6000U / gpro, adjust the pH to 7.5, and the reaction temperature 50°C, enzymatic hydrolysis time 4h, after enzymolysis, inactivate the enzyme at 90-100°C for 30min, cool to room temperature and centrifuge to obtain sturgeon bone type II collagen peptide so...

Embodiment 2

[0023] Choose fresh sturgeon cartilage as the raw material for collagen extraction, wash it, chop it up, add 10 times the volume (w / v) of 0.08mol / L NaOH solution and stir for 3 hours, rinse with running water until neutral, homogenate, add 5 Double the volume of purified water, treat at 120°C for 50 minutes, cool down to 65°C for extraction for 4 hours, centrifuge at 6000 rpm, add 2 times the volume of water to the residue and continue to extract at 65°C for 1 hour, remove the residue by centrifugation at 6000 rpm, and combine the two extractions solution, which is the sturgeon bone type II collagen extract; adjust the concentration of collagen in the concentrated solution to 2%, add alkaline protease to it, the addition amount is 8000U / gpro, adjust the pH to 9, and the reaction temperature is 50°C. The enzymolysis time is 4 hours. After the enzymolysis, inactivate the enzyme at 90-100°C for 30 minutes, cool to room temperature and centrifuge to obtain the sturgeon bone type II...

Embodiment 3

[0025] Frozen sturgeon cartilage and sturgeon skull were selected as raw materials for collagen extraction, thawed and washed, chopped, added 10 times the volume (w / v) of 0.1mol / L NaOH solution, stirred for 2 hours, rinsed with running water until neutral , homogenate, add 4 times the volume of purified water, treat at 125°C for 60 minutes, cool down to 60°C for extraction for 4 hours, centrifuge and filter at 8000 rpm, add 2 times the volume of water to the residue and continue to extract at 60°C for 1 hour, centrifuge at 8000rpm to remove the residue , the combined extract twice is the sturgeon bone type II collagen extract; adjust the concentration of collagen in the concentrated solution to 5%, add trypsin and neutral protease to it, the addition amount is 8000U / gpro respectively, Adjust the pH to 7.5, the reaction temperature is 50°C, and the enzymatic hydrolysis time is 3 hours. After the enzymolysis is completed, the enzyme is inactivated at 90-100°C for 30 minutes, cool...

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PUM

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Abstract

The invention belongs to the technical field of preparation of functional polypeptide, and particularly relates to a preparation method of sturgeon bone II-type collagen peptide. The preparation method sequentially comprises the following steps: preparing sturgeon bone II-type collagen, preparing sturgeon bone II-type collagen peptide, desalting, concentrating, separating and purifying. The finally prepared II collagen peptide is low-molecular weight sturgeon cartilage II collagen peptide, and has the hydrolysis degree of 9-17% and peptide chain length range of 3-24 amino acids. The collagen peptide prepared by the preparation method is further rich in chondroitin and calcium, and has a good joint health-caring function; and in addition, the preparation method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of preparation of functional polypeptides, and in particular relates to a preparation method of sturgeon bone type II collagen peptide. Background technique [0002] Our country is extremely rich in sturgeon resources. There are a total of 27 species of sturgeon in the world, and 8 species can be found in my country, among which Chinese sturgeon, Sturgeon's sturgeon and Dabry's sturgeon are the three species with the largest proportion. Sturgeon is full of treasures. Collagen can be extracted from fish skin and swim bladder, and high-quality fish protein peptides can be extracted from fish meat. Sturgeon not only has rich nutritional value, but also unique medical and health value, known as "shark fin, sturgeon bone". Sturgeon has a large body, and cartilage accounts for a relatively large proportion (about 5.7% of the body). Except for the partially ossified fin and a piece of true bone in the head, the re...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K14/78
Inventor 郑平安王加斌夏志国袁军岳王高峰袁法磊袁超璐滕芳芳唐嘉
Owner HAILISHENG GROUP
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