Method for extracting undenatured type II collagen from squid cartilage

A squid cartilage and collagen technology, which is applied in the field of type II collagen extraction, can solve problems such as failure to make good use of type II collagen, and achieve the effects of complete deashing, subsequent operations, and improved extraction purity.

Inactive Publication Date: 2015-12-02
SHANGHAI FISHERIES RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since chondroitin sulfate, a polysaccharide contained in cartilage, is a health food that can effectively improve joint pain, type II collagen is not well utilized when extracting polysaccharides

Method used

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  • Method for extracting undenatured type II collagen from squid cartilage

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Comparison scheme
Effect test

Embodiment 1

[0036] A method for extracting non-denatured type II collagen from squid cartilage, comprising:

[0037] (1) Take the fresh squid to peel off the cartilage, remove the attached muscles, and freeze-dry it under vacuum at -40°C;

[0038] (2) the freeze-dried squid cartilage is pulverized into 100 mesh particle diameter cartilage powder;

[0039] (3) Weigh 100g squid cartilage powder, suspend with 3000mL pH2.0HCl solution, stir slowly at 4°C for 24h, centrifuge at 10000r / min for 30min at 4°C, and remove the supernatant;

[0040] (4) The precipitate was fully washed with deionized water at 4°C, centrifuged at 4°C and 10000r / min, and the precipitate was retained;

[0041] (5) Suspend the precipitate with 3000mL0.5 MEDTA solution (pH8.0), stir slowly at 4°C for 24h, centrifuge at 10000r / min at 4°C for 30min, and remove the supernatant;

[0042] (6) The precipitate was fully washed with deionized water at 4°C, centrifuged at 4°C and 10000r / min, and the precipitate was retained;

...

Embodiment 2

[0051] A method for extracting non-denatured type II collagen from squid cartilage, comprising:

[0052] (1) Take the fresh squid to peel off the cartilage, remove the attached muscles, and freeze-dry it under vacuum at -20°C;

[0053] (2) the freeze-dried squid cartilage is pulverized into 200 mesh particle diameter cartilage powder;

[0054] (3) Weigh 100g of squid cartilage powder, suspend in 5000mL of pH3.0HCl solution, stir slowly at 4°C for 24h, centrifuge at 10,000r / min at 4°C for 30 minutes, and remove the supernatant;

[0055] (4) The precipitate was fully washed with deionized water at 4°C, centrifuged at 4°C and 10000r / min, and the precipitate was retained;

[0056] (5) Suspend the precipitate with 5,000 mL of 1.0 MEDTA solution (pH 8.0), stir slowly at 4°C for 24 hours, centrifuge at 10,000 r / min for 30 minutes at 4°C, and remove the supernatant;

[0057] (6) The precipitate was fully washed with deionized water at 4°C, centrifuged at 4°C and 10000r / min, and the ...

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Abstract

The invention provides a method for extracting undenatured type II collagen from squid cartilage. The method includes: freeze-drying squid cartilage; crushing the freeze-dried squid cartilage, and sieving to obtain cartilage powder; suspending the cartilage powder in HCL solution, EDTA solution and NaOH solution to obtain precipitate; adding acetic acid solution and protease into the precipitate, stirring, centrifuging and collecting supernate; adding saturated NaCl solution into the supernate until NaCl concentration is 1-1.5M, allowing standing for 16-24h, centrifuging, and removing supernate to obtain first precipitate; dissolving the first precipitate by using acid, performing dialysis, and performing freeze-drying to obtain the undenatured type II collagen. The purity of the undenatured type II collagen acquired by extracting is not lower than 95%.

Description

technical field [0001] The invention relates to the technical field of extracting type II collagen, in particular to a method for extracting non-denatured type II collagen from squid cartilage. Background technique [0002] Type II collagen (collagentype II, CII) is one of the main components of cartilage, which has three α 1 The chains are combined in the form of triple helices and cross-linked through covalent bonds to form a special three-dimensional network structure, which plays an important role in maintaining the integrity of the cartilage structure. Type II collagen is a protein that is tightly combined with polysaccharides, which can promote the deposition of inorganic minerals such as calcium and phosphorus on bones, repair tissues, and interact with proteoglycans in tissues to make cartilage and ligaments elastic. [0003] Type II collagen is an autoantigen, anti-collagen antibody and collagen-specific T cells of rheumatoid arthritis (RA), which play an important...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 郭休玉何兰王南平
Owner SHANGHAI FISHERIES RES INST
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