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Preparation method for I/II collagen double-layer composite collagen membrane

A double-layer composite and collagen membrane technology, applied in coatings, medical science, prostheses, etc., can solve problems such as unsatisfactory clinical effects and inability to achieve hyaline cartilage repair, and achieve dense pore size, uniform distribution, and a good growth environment. Effect

Active Publication Date: 2013-10-09
张仲文 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used treatment methods include arthroscopic debridement, articular cartilage drilling and fracture, and autologous or allogeneic bone-cartilage transplantation, but the clinical effect is not satisfactory, and none of them can achieve hyaline cartilage repair.

Method used

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  • Preparation method for I/II collagen double-layer composite collagen membrane
  • Preparation method for I/II collagen double-layer composite collagen membrane
  • Preparation method for I/II collagen double-layer composite collagen membrane

Examples

Experimental program
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Effect test

Embodiment 1

[0045] The method for preparing I / II collagen bilayer composite collagen film comprises:

[0046] (1) Preparation of type I collagen membrane:

[0047] (1) Wash 10 g of bovine Achilles tendon 3 times with ultrapure water, trim to remove impurities, wash, soak the tissue in 95% ethanol for 10 minutes, and wash 5 times with ultrapure water;

[0048] (2) mash the bovine Achilles tendon of step (1) with tissue scissors, soak in 0.05mol / L acetic acid for 10min;

[0049] (3) Bovine Achilles tendon swells in acetic acid, becomes transparent jelly, homogenizes, and operates at 4°C;

[0050] (4) Put the bovine Achilles tendon of step (3) into a beaker, add 400ml 0.05mol / L acetic acid and 200mg pepsin, stir at 4°C for 72h for enzymolysis;

[0051] (5) Centrifuge the enzymatic hydrolysis solution in step (4) at 15,000 r / m for 30 minutes, collect the supernatant, adjust the pH value to neutral, and operate at 4°C;

[0052] (6) Centrifuge the solution in step (5) at 12000r / m for 30min, co...

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Abstract

The invention relates to a preparation method for an I / II type double-layer composite collagen membrane and the I / II type double-layer composite collagen membrane prepared by using the preparation method. The preparation method comprises the following steps: 1, extracting type-I collagen and preparing a type-I collagen membrane; 2, extracting type-II collagen and preparing a type-II collagen membrane; and 3, with the type-I collagen membrane as a bottom layer and the type-II collagen membrane as a top layer, carrying out crosslinking so as to form the I / II type double-layer composite collagen membrane. The I / II type double-layer composite collagen membrane provides a good growth environment for chondrocytes and has a good cartilage repair effect.

Description

technical field [0001] The invention relates to a preparation method of a type I / II double-layer composite collagen film and a type I / II double-layer composite collagen film prepared by the method. Background technique [0002] Osteoarthritis, sports injuries, osteochondritis dissecans and many other causes can lead to articular cartilage damage. Articular cartilage itself has no blood supply, lymphatic drainage, and innervation. It is a highly differentiated tissue. The division ability of mature cartilage tissue is very limited, and it is difficult to repair once damaged. Therefore, how to solve the problem of articular cartilage repair has become increasingly important. At present, the commonly used treatment methods include arthroscopic debridement, articular cartilage drilling and fracture, and autologous or allogeneic bone-cartilage transplantation, but the clinical effect is not satisfactory, and hyaline cartilage repair cannot be achieved. With the development and ...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/28
Inventor 张仲文刘爱兵靳凯锋许云飞
Owner 张仲文
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