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Fluorescein rhodamine B double-Schiff alkali compound ultraviolet molecular probe for Fe<3+> detection as well as synthesis and application thereof

A technology of molecular probes and base compounds, which is applied in the measurement of color/spectral properties, luminescent materials, organic chemistry, etc., can solve the problems that ultraviolet spectroscopy is easily interfered by other metal ions, and the detection instruments of fluorescence spectroscopy are expensive. The effect of high rate, low cost and strong anti-interference ability

Inactive Publication Date: 2017-11-03
QIQIHAR UNIVERSITY
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Problems solved by technology

[0004] The present invention is to solve the existing detection Fe 3+ The fluorescence spectrometry detection instrument is expensive, and the ultraviolet spectrometry is easily interfered by other metal ions. It is provided for Fe 3+ Detection of Fluorescein Rhodamine B Bis-Schiff Base Compound UV Molecular Probe and Its Synthesis and Application Using a UV Spectrophotometer to Realize Fe 3+ detection

Method used

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  • Fluorescein rhodamine B double-Schiff alkali compound ultraviolet molecular probe for Fe&lt;3+&gt; detection as well as synthesis and application thereof
  • Fluorescein rhodamine B double-Schiff alkali compound ultraviolet molecular probe for Fe&lt;3+&gt; detection as well as synthesis and application thereof
  • Fluorescein rhodamine B double-Schiff alkali compound ultraviolet molecular probe for Fe&lt;3+&gt; detection as well as synthesis and application thereof

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specific Embodiment approach 1

[0024] Specific embodiment one: the present embodiment is used for Fe 3+ The structural formula of the detected fluorescein rhodamine B double Schiff base compound ultraviolet molecular probe is:

[0025]

specific Embodiment approach 2

[0026] Specific embodiment two: specific embodiment one described for Fe 3+ The synthetic method of the fluorescein rhodamine B bis-Schiff base compound ultraviolet molecular probe of detection is as follows:

[0027] 1. According to the molar ratio of monoimino fluorescein aldehyde and rhodamine B hydrazine as 1: (1 ~ 3), add monoimino fluorescein aldehyde and rhodamine B hydrazine to alcohol solvent respectively, and heat to Boiling and reflux reaction for 3 to 10 hours, after the reaction is completed, cool and concentrate to obtain a mixture;

[0028] 2. The mixture is separated by gradient elution of column chromatography, wherein the column is filled with 200-300 mesh silica gel, and the eluent is a mixture of petroleum ether and ethyl acetate to obtain 3+ Detection of Fluorescein Rhodamine B Bis-Schiff Base Compound UV Molecular Probe.

specific Embodiment approach 3

[0029] Embodiment 3: The difference between this embodiment and Embodiment 2 is that the alcohol solvent in step 1 is methanol, ethanol, propanol, isopropanol or butanol; the others are the same as Embodiment 2.

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Abstract

The invention discloses a fluorescein rhodamine B double-Schiff alkali compound ultraviolet molecular probe for Fe<3+> detection as well as synthesis and application thereof, and relates to a Fe<3+> probe as well as synthesis and application thereof. The technical problems that an apparatus required by an existing Fe<3+> fluorescent molecular probe is expensive and the ultraviolet probe is susceptible to interference are solved. A structural formula of the ultraviolet molecular probe disclosed by the invention is that the formula (1) is shown in the description. A preparation method comprises the following steps: carrying out condensation reaction between single-imino fluorescein aldehyde and rhodamine B hydrazine, and carrying out column chromatography separation on a mixed solvent of petroleum ether and ethyl acetate to obtain the ultraviolet molecular probe; dissolving the probe into a mixed solution of DMF (N,N-Dimethyl Formamide) and an HEPES (2-[4-(2-Hydroxyethyl)-1-Piperazinyl] Ethanesulfonic Acid) buffer solution; detecting ultraviolet spectral absorbance values before and after addition of to-be-detected samples, and judging that a sample solution contains Fe<3+> if the absorbance values of parts on which the wavelengths are 323nm and 358nm are increased. The method is simple and quick.

Description

technical field [0001] The present invention relates to Fe 3+ Synthesis and application of ultraviolet molecular probes for detection. Background technique [0002] Iron is widely distributed in nature and is one of the most abundant metal ions in organisms. It plays a very important role in biochemical processes such as oxygen transport, electron transfer, DNA synthesis and repair, and photosynthesis. It is indispensable for organisms. One of the missing metal elements. Iron deficiency and excess can induce corresponding diseases. For example, iron deficiency can lead to insufficient oxygen delivery to cells, leading to diseases such as anemia, hemochromatosis, liver damage, diabetes and cancer. Currently, for Fe 3+ Most of the detected chemical sensors are fluorescent probes. That is, the detection of metal ions is realized by using the change of the fluorescence spectrum when the fluorescent chemical probe interacts with the metal ions. For example, the Chinese paten...

Claims

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Application Information

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IPC IPC(8): C07D519/00C09K11/06G01N21/33
CPCC07D519/00C09K11/06C09K2211/1007C09K2211/1029C09K2211/1088G01N21/33
Inventor 阚伟李少鑫卜凡强赵冰李慧婷阚宏泽刘双义
Owner QIQIHAR UNIVERSITY
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