Method for detecting urea based on metal organic framework material fluorescent sensor

A metal-organic framework and fluorescent sensor technology, applied in the field of analysis and detection, can solve the problems of large interference of coexisting substances, high false positive rate, cumbersome and time-consuming operation, etc., and achieve high specificity

Inactive Publication Date: 2016-10-12
SHENZHEN POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the shortcomings of the existing urea detection technology, such as large interference of coexisting substances, low sensitivity, cumbersome and time-consuming operation, and high false positive rate, and to apply metal-organic framework materials to

Method used

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  • Method for detecting urea based on metal organic framework material fluorescent sensor
  • Method for detecting urea based on metal organic framework material fluorescent sensor
  • Method for detecting urea based on metal organic framework material fluorescent sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 (the inventive method detects urea)

[0035] Synthesis of zirconium-containing organic framework material UiO-66-NH 2 , the process is as follows:

[0036] Step 1: ZrOCl 2 2.5H 2 O (0.645g, 2mmol), BDC-NH 2 (0.362g, 2mmol), benzoic acid (4.885g, 40mmo) and concentrated hydrochloric acid (12mol / L, 0.347mL) were ultrasonically dissolved in 34mL DMF, after dissolution, the solution was transferred to a 100mL Teflon-lined high pressure reaction kettle, put it in an oven, react at 120°C for 24 hours, then cool to room temperature, filter the product to obtain a yellow powder product, rinse with 20mL DMF and 10mL acetone three times, and dry at 80°C. spare.

[0037] Step 2: Put the solid powder obtained in step 1 in 20mL DMF and centrifuge for 12 hours (replace the solvent every 4 hours), then soak it in 10mL ethanol for 1 day (replace the solvent every 8 hours), and then dry it in a vacuum oven. Dry at 80°C for 20 hours to obtain the activated UiO-66-NH 2...

Embodiment 2

[0043] Embodiment 2 (application of the inventive method in detecting urea and other common coexisting substances)

[0044] For evaluating the specificity of the method of the present invention, common coexistence substance such as calcium ion Ca in the food is chosen 2+ , Potassium ion K + , Sodium ion Na + , glutamic acid Glu, bovine serum albumin BSA, lactose Lac and casein Casein, these substances are measured according to the detection steps described in Example 1, and the respective fluorescent signals are recorded and compared, the results are as follows image 3 shown. Since the target of urease is urea, the fluorescence signal of urea (6.0mg / L) is the strongest, and when the concentration of other coexisting substances (300mg / L) is 50 times that of urea, the fluorescence intensity is one-third of the urea signal or even lower. This result indicates that based on the metal-organic framework material UiO-66-NH 2 The method for detecting urea with a fluorescent sens...

Embodiment 3

[0045] Embodiment 3 (the inventive method is applied to the detection of urea in liquor)

[0046] Five liquor samples were purchased from local supermarkets, and 25 mL of the five liquor samples were put into five round-bottomed flasks, placed on a rotary evaporator, and evaporated to dryness at a temperature of 80 °C and a speed of 70-80 r / min. After the wine sample is completely evaporated to dryness, remove the flask, cool to room temperature, add 5 mL of distilled water, shake fully to wash out the residue, and filter the eluate through a 0.22 μm water phase filter membrane to obtain five parts of the treated sample liquid. Each sample was detected three times in parallel.

[0047] According to the analysis steps described in Example 1, the five processed liquor samples were detected respectively, and urea was detected in all five liquor samples, and the results are shown in Table 1.

[0048] Add different concentration (0.1mg / L~0.3mg / L) urea respectively in five parts of...

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Abstract

The invention provides a method for detecting urea based on a metal organic framework material fluorescent sensor. The method comprises the following steps that 1, a zirconium metal organic framework material UiO-66-NH2 is prepared; 2, the zirconium metal organic framework material UiO-66-NH2 is uniformly ground, ground powder is weighed and subjected to ultrasonic oscillating to be dispersed in distilled water, and a UiO-66-NH2 material solution is obtained and stored at 4 DEG C in a lucifugal mode for use; 3, urease is taken to be dissolved in a hydroxyethyl piperazine ethanesulfonic acid buffer solution, and a urease solution is prepared and stored at 4 DEG C in a lucifugal mode for use; 4, the UiO-66-NH2 material solution and the urease solution are taken separately, fully mixed and kept for 10 min at 37 DEG C, and a fluorescence signal is detected; 5, urea with different concentrations is added, incubation is conducted for 30 min at 37 DEG C, and fluorescence signals in the presence of the urea with different concentrations are detected; 6, qualitative and quantitative analyses are conducted on urea in a sample according to the fluorescence signals and a working curve. The method for detecting urea based on the metal organic framework material fluorescent sensor is a urea detection method which is rapid, accurate, reliable, sensitive and high in specificity.

Description

technical field [0001] The invention relates to the fields of analysis and detection of food safety, water quality monitoring, etc., and in particular to a method for detecting urea by utilizing the fluorescence characteristics of metal organic framework materials. Background technique [0002] Urea is the final product of amino acid metabolism. When the content of urea exceeds its own absorption capacity, it will cause harm to the liver, kidneys, and alveoli of the body. In recent years, in the processing and production of milk, bean sprouts and other foods, the problem of illegal addition of urea to increase production and income has emerged endlessly. Incidents of illegal addition of urea to livestock feed such as cattle and sheep have also occurred from time to time, causing livestock deaths. At the same time, there are also excessive urea in the environment. The high concentration of urea in bathing beaches and swimming pools will also affect people's health. Therefore...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 蒋晓华丁文捷
Owner SHENZHEN POLYTECHNIC
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