Nucleic acid protective liquid for long-term storage and transportation of tissue sample in constant temperature condition

A technology for nucleic acid protection and normal temperature, which is applied in the application field of nucleic acid protection solution and its storage and transportation of tissue samples at normal temperature, which can solve the problems of operation error and high cost of low temperature storage, and achieve simple operation, long storage time and good storage effect Good results

Active Publication Date: 2015-12-16
无锡泛生子生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has relatively large limitations. First, the cost of low temperature storage is high; second, it is reflected in the demand for special equipment, such as liquid nitrogen tanks. If freshly collected samples cannot be placed in liquid nitrogen immediately, nucleic acid Molecules may be degraded, or there may be other influencing factors that change the state of the nucleic acid, causing operational errors in subsequent experiments

Method used

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  • Nucleic acid protective liquid for long-term storage and transportation of tissue sample in constant temperature condition
  • Nucleic acid protective liquid for long-term storage and transportation of tissue sample in constant temperature condition
  • Nucleic acid protective liquid for long-term storage and transportation of tissue sample in constant temperature condition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take the following components in the formula amount and add them to the sterilized container:

[0032] D-glucose 4100mg, 4-hydroxyethylpiperazineethanesulfonic acid 2500mg;

[0033] NaCl3700mg, NaHCO 3 2200mg, NaH 2 PO 4 110mg, KCl400mg, CaCl 2 180mg, MgCl 2 85 mg;

[0034] L-lysine hydrochloride 135mg, L-histidine hydrochloride 85mg, L-isoleucine 90mg, L-leucine 110mg, L-threonine 85mg, L-phenylalanine 70mg, L-valine 85mg, L-tyrosine 75mg;

[0035] Choline chloride 3mg, folic acid 5mg, nicotinamide 3mg, inositol 5mg, vitamin A0.15mg, vitamin H0.2mg, vitamin E0.15mg;

[0036] Holo-Human Transferrin 90mg, Bovine Serum Albumin 110mg, Glycine Dipeptide 400mg, Insulin 20mg;

[0037] Epinephrine 1.5mg, linoleic acid 4.0mg, linolenic acid 2.5mg, progesterone 0.13mg;

[0038] Penicillin 0.9×10 5 Unit, Streptomycin 100mg, Amphotericin B 0.2mg;

[0039] Add water to make up to 1L.

Embodiment 2

[0041] Take the following components in the formula amount and add them to the sterilized container:

[0042] D-glucose 4300mg, 4-hydroxyethylpiperazineethanesulfonic acid 2400mg;

[0043] NaCl3900mg, NaHCO 3 2000mg, NaH 2 PO 4 125mg, KCl350mg, CaCl 2 210mg, MgCl 2 70 mg;

[0044]L-lysine hydrochloride 150mg, L-histidine hydrochloride 75mg, L-isoleucine 110mg, L-leucine 90mg, L-threonine 95mg, L-phenylalanine 60mg, L-valine 95mg, L-tyrosine 65mg;

[0045] Choline chloride 5mg, folic acid 3mg, nicotinamide 5mg, inositol 3mg, vitamin A0.2mg, vitamin H0.15mg, vitamin E0.2mg;

[0046] Holo-110mg of human transferrin, 90mg of bovine serum albumin, 450mg of glutamic dipeptide, 15mg of insulin;

[0047] Epinephrine 2.0mg, linoleic acid 3.5mg, linolenic acid 3.0mg, progesterone 0.07mg;

[0048] Penicillin 1.0×10 5 Unit, Streptomycin 90mg, Amphotericin B 0.3mg;

[0049] Add water to make up to 1L.

Embodiment 3

[0051] Take the following components in the formula amount and add them to the sterilized container:

[0052] D-glucose 4200mg, 4-hydroxyethylpiperazineethanesulfonic acid 2450mg;

[0053] NaCl3800mg, NaHCO 3 2083 mg, NaH 2 PO 4 118mg, KCl380mg, CaCl 2 190mg, MgCl 2 73.2 mg;

[0054] L-Lysine Hydrochloride 138mg, L-Histidine Hydrochloride 79.5mg, L-Isoleucine 100mg, L-Leucine 100mg, L-Threonine 90mg, L-Phenylalanine 62.5 mg, L-valine 90mg, L-tyrosine 68mg;

[0055] Choline chloride 4mg, folic acid 4mg, nicotinamide 4mg, inositol 4mg, vitamin A0.19mg, vitamin H0.19mg, vitamin E0.19mg;

[0056] Holo-Human Transferrin 100mg, Bovine Serum Albumin 100mg, Propandipeptide 435mg, Insulin 18.8mg;

[0057] Epinephrine 1.9mg, linoleic acid 3.8mg, linolenic acid 2.8mg, progesterone 0.1mg;

[0058] Penicillin 1.0×10 5 Unit, Streptomycin 100mg, Amphotericin B 0.25mg;

[0059] Add water to make up to 1L.

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Abstract

The invention discloses a nucleic acid protective liquid and an application thereof for long-term storage and transportation of a tissue sample at constant temperature. The nucleic acid protective liquid comprises the following components: D-glucose, 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid, inorganic salt, amino acids, choline chloride, folic acid, nicotinamide, inositol, vitamin A, vitamin H, vitamin E, Holo-human transferrin, bovine serum albumin, N-(2)-L-alanyl-L-glutamine, insulin, adrenalone, linoleic acid, linolenic acid, progesterone, penicillin, streptomycin, amphotericin B and water. The nucleic acid protective liquid is low in cost. By using the nucleic acid protective liquid, nucleic acid in the tissue sample is long in storage time at room temperature, and the obtained nucleic acid is high in concentration and great in total amount. A preparation method for the nucleic acid protective liquid is simple and fast.

Description

technical field [0001] The invention relates to a nucleic acid protection solution and its application, in particular to a nucleic acid protection solution and its application in preserving and transporting tissue samples at normal temperature. Background technique [0002] Nucleic acid is an important macromolecular compound unique to organisms, which widely exists in various biological cells. Nucleic acid carries biological genetic information, and accurate detection of nucleic acid is an important aspect of molecular biology sample analysis. However, nucleic acids can be degraded under many conditions. External physical factors that cause nucleic acid degradation such as temperature, humidity, ultraviolet light, etc.; chemical factors such as pH value, hydrolysis reaction, oxidation reaction, etc.; biological factors such as enzymatic hydrolysis and microbial infection. [0003] For tissue samples, the current way to store samples is mainly to store samples directly at ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 阎海焦雨辰王晓月王思振熊梓锴
Owner 无锡泛生子生物科技有限公司
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