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Chromatographic separation method of trehalose and maltose

A chromatographic separation and trehalose technology, applied in the field of biochemical separation, can solve the problems of difficult construction of special chromatographic media, high energy consumption, high equipment requirements, etc., and achieve the effect of low cost, high product purity and high separation degree

Active Publication Date: 2015-04-01
EAST CHINA UNIV OF SCI & TECH
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The application number is CN200710013078.7, and the Chinese patent application with publication number CN101230407A is a method for improving the extraction yield of trehalose. The disclosed technology mainly uses hydrogenation technology to convert maltose into maltitol, and then utilizes chromatographic separation technology to convert maltitol and Trehalose separation, this method is more complicated to operate, requires the use of expensive catalysts and high temperature and high pressure conditions, requires high equipment and consumes a lot of energy
At present, there are still no reports on the direct and efficient separation of trehalose and maltose by chromatographic technology at home and abroad. The main reason is that it is very difficult to construct a special chromatographic medium filled with a significant difference in the adsorption capacity of trehalose and maltose in the chromatographic column.

Method used

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  • Chromatographic separation method of trehalose and maltose
  • Chromatographic separation method of trehalose and maltose

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Experimental program
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Effect test

Embodiment 1

[0034] The feed aqueous solution of maltose and trehalose (mass percentage 1% maltose, 99% trehalose) (the mass percentage concentration of the total content of maltose and trehalose is 1%), and the injection diameter-to-height ratio is 1:1 to fill calcium-type cations Chromatographic resin chromatographic column, the column temperature is controlled at 90°C, the feed flow rate is 0.01BV / h; deionized water is used as the eluent, the ratio of material to water is 1:2.5, and the column temperature is controlled at 90°C (the column temperature is controlled during the whole separation process keep constant) and the flow rate is 0.025BV / h; the eluate and residual liquid are collected respectively to obtain aqueous solutions of maltose and trehalose, and the separation rate of the two substances is 98.0%.

[0035] The calculation method of the above separation rate is:

[0036] Separation rate: K=(H-h) / H×100%, K—separation rate of peak height, H—peak height of small peak, h—height ...

Embodiment 14

[0041] Filter the trehalose material liquid produced by maltase conversion through membrane filtration (the filtration conditions of membrane filtration are: microfiltration membrane (CA membrane), temperature: 25-30°C, operating pressure: 0.01-0.2MPa), and concentrate through the membrane (Membrane concentration conditions are: nanofiltration membrane (NF-1-D-4040), temperature 25-30°C, pressure 0.5-1MPa) to make an aqueous solution with a concentration of 1% (w / w), and the injection diameter-to-height ratio is 1: 1 Fill a calcium-type cation chromatography resin column, control the column temperature at 90°C, and feed the flow rate at 0.01BV / h; use deionized water as the eluent, the ratio of material to water is 1:2.5, and control the column temperature at 90°C (column Temperature remains constant throughout the separation process) and the flow rate is 0.025BV / h conditions for eluting; respectively collect the eluate and residual liquid to obtain the aqueous solutions of the ...

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Abstract

The invention relates to a chromatographic separation method of trehalose and maltose, and belongs to the technical field of biochemical separation. The method comprises the following steps: carrying out membrane filtration, centrifugal clarification or plate and frame filtration on a mycose feed liquid produced by maltase transformation, and preparing a 1%-71% (w / w) water solution through heat concentration or membrane concentration; feeding to a chromatographic column filled with a special chromatographic medium at 20-90 DEG C, eluting with water as an eluting agent, and respectively collecting an eluting liquid and a residual liquid, so as to obtain two products pure mycose and maltase, and thus efficient separation of the mycose and the maltase is achieved. Compared with the prior art, the chromatographic separation method has the advantages of low cost, high separation efficiency and the like, and is simple to operate and environmental friendly.

Description

technical field [0001] The invention relates to a method for separating trehalose and maltose by using chromatographic technology, and belongs to the technical field of biochemical separation. Background technique [0002] Trehalose is a non-reducing sugar composed of two glucose molecules with α,α-1,1 glycosidic bonds. Due to the presence of non-reducing ends of trehalose, it has the function of protecting biological cells and biologically active substances from damage in adverse environments. It is widely used in food, medicine, cosmetics, agriculture and other industries. It can be used as a food additive and sweetener in the food industry while maintaining food storage life; in the pharmaceutical industry, it can replace plasma protein as a stabilizer for biologically active substances such as blood products and vaccines; in cosmetics, it can be used as a super sunscreen moisturizing factor, Used in skin care products and lipsticks, etc.; used for seed preservation in c...

Claims

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Application Information

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IPC IPC(8): C07H3/04C07H1/06C13K7/00
Inventor 赵黎明何灯军邱勇隽周家春夏泉鸣
Owner EAST CHINA UNIV OF SCI & TECH
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