53results about How to "Increase total nitrogen" patented technology

The invention discloses a special compound fertilizer for watermelons and a preparation method of the special compound fertilizer. The special compound fertilizer for watermelons comprises the following substances in parts by weight: 10-26 parts of high-quality candy pomace, 2-4 parts of fulvic acid, 1-4 parts of adhesive, 10-28 parts of urea, 8-20 parts of monoammonium phosphate, 2-15 parts of potassium sulfate, 20-30 parts of ammonium sulfate, 1-4 parts of trace elements, 2-5 parts of charcoal and 3-5 parts of traditional Chinese medicine. The preparation method comprises the following steps: grinding the previous substances, uniformly mixing, and granulating, thereby obtaining the special compound fertilizer for watermelons. The special compound fertilizer for watermelons disclosed by the invention has the quick effect of an inorganic fertilizer and also has the long-acting and slow-release effects of an organic fertilizer, the volume weight of the soil is reduced, the granular structure in the soil is increased, the pH value of the soil is regulated, the special compound fertilizer has the effects of effectively expelling insects and sterilizing, the sugar content of the watermelon fruits is improved, and the solid content is increased, so that the yield and quality of the watermelons are improved. Meanwhile, the orchard soil can be improved, the environment is protected, and the special compound fertilizer has wide application prospects in green fruit production.

The invention provides a method for composting and returning crop straws to a field. The method comprises the following steps: digging a pit in suitable size at a corner of the field; stacking the straws in the pit in a layering way and treading down the straws; spreading a right amount of straw ageing agents and urea in each layer; after finishing stacking the straws, uniformly covering the top of the stack with the soil dug from the pit; after composting for several months, digging out the rotten straws from the pit and returning the straws to the field after harvesting crops in next season; and uniformly spreading the rotten straws in the field and using the rotten straws as base fertilizers, wherein organic matters got after the straws are rotten become clean green fertilizers. Under the condition of using the fertilizers, the hardened land becomes soft and loose, the fertilizer cost of peasants for farming is saved, and the environmental pollution problem caused by burning the straws is relieved.

The invention discloses a planting method for increasing rice yield by returning rape as green manure during the flowering stage. The method comprises the steps of: ditching a rice field as furrows in late October, and directly planting rape seeds without tillage; spraying phytocide once only during the (3-4)-leaf stage in growth of the rape, and further fertilizing the rape once during the (3-4)-leaf stage and the peduncle-growing period respectively; cutting off the rape during the flowering stage, and mechanically turning over the soil to restore the field after irrigating the soil; planting the stubbled rice in ridge furrows, controlling water irrigation during the growth period, retaining the shallow water layers during the seedling establishment stage, the disease and pest prevention and treatment stage and the head sprouting and flowering stage, keeping the ditch to have water while keeping the furrows to have no water on surface during a period except the periods above, wherein the fertilizing amount of the rice is reduced by 15%. The planting method for increasing rice yield by returning rape as green manure during the flowering stage combines rice production with biologic cultivation and fertilization, not only reduces the fertilizing amount of the rice but also increases the yield of the rice and improves the physical properties of the soil of the rice field, so theplanting method is characterized by saving labor and cost and achieving high yield and high efficiency.

The invention provides a microbial agent YF, wherein the microbial agent YF comprises four strains. The four strains are Bacillus soniensis B-2, Bacillus paralicheniformis B-26, Bacillus soniensis N-16 and Bacillus pumilus N-17. The four strains are all preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation date is March 5,2020, wherein the preservation number of the Bacillus soniensis B-2 is CGMCC NO.19454, the preservation number of the Bacillus paralicheniformis B-26 is CGMCC NO.19449, the preservation number of the Bacillus sorola N-16 is CGMCC NO.19448, and the preservation number of the Bacillus pumilus N-17 is CGMCC NO.19450. The microbial inoculum provided by the invention can effectively prevent the corn stalk rot in saline soil, improve soil quality of the saline soil, and obviously promote growth of corns.

The invention discloses a technology for preparing bio-fertilizer from garbage. The technology provided by the invention adopts raw materials of 45% of garbage, 35% of turf, 13% of rice husk powder, 5% of danty, 1.5% of rice bran and 0.5% of a composite microbial inoculant, and comprises carrying out composting through uniformly mixing the crushed garbage, the crushed turf, the crushed rice bran and the crushed danty to obtain mixed raw materials, carrying out micropropagation of the composite microbial inoculant which is a home-made composite microbial inoculant, uniformly mixing the composite microbial inoculant subjected to micropropagation and the mixed raw materials, and piling up the mixture by a turning ventilation method so that the mixture forms a pile having the length of 5m, the width of 4m and the height of 1.5m. The technology provided by the invention selects a novel strain, utilizes novel inoculants, optimizes production processes, realizes preparation of organic bio-fertilizer satisfying the national standard through selection of a raw material ratio, confirms field application effects of the organic bio-fertilizer, lays a firm foundation for popularization of the organic bio-fertilizer and wide application of the organic bio-fertilizer in production, and is suitable for the technical field of environmental protection.

The invention relates to a sowing technique, in particular to a stub no-tillage mechanical sowing cultivation method. The wheat stub no-tillage rape cultivation method comprises the steps of mechanically harvesting wheat, mixing selected rape seeds, chemical fertilizers and sheep dung, sowing rape seeds, performing rape field management, harvesting and the like. The method has the advantages of increasing soil moisture, reducing wind erosion and water and soil loss, regulating ground temperature, increasing nutrients in soil, improving activity of soil enzymes, preventing lodging, increasing forage grass and the like.

The invention provides a preparation method of a compound photosynthetic bacterial fertilizer. An ectothiorhodospira shaposhnikovii strain of chromatiaceae and a rhodocyclus tenuis strain of rhodospirillaceae are subjected to culture steps such as seed activation culture, seed culture, illumination culture in an anaerobic fermentation tank and the like, and the high-concentration compound photosynthetic bacterial fertilizer containing ectothiorhodospira shaposhnikovii and rhodocyclus tenuis is obtained. The compound photosynthetic bacterial fertilizer can realize disease prevention, fertilization, medicinal residue degradation and soil remediation, takes effect fast and has a stable effect, and the use amount is small. The preparation method of the compound photosynthetic bacterial fertilizer obtained after mixed culture of ectothiorhodospira shaposhnikovii and rhodocyclus tenuis which are high in growth speed, high in thallus content and short in culture time is provided.

This invention relates to a fermental shoyu and preparation of levure species in the process of producing levure from shoyu product. The preparation of saccharifying levure of increasing incense, its characteristic is that including following steps: 1). Preparation of esterifying red rice; 2): preparation of fermental red rice;3): preparation of black levure;4): preparation of saccharifying levure of increasing incense: a). preparation of saccharifying levure of increasing incense which is used for shoyu: it is composed of fermental red rice, esterifying red rice and black levure, the above-mentioned fermental red rice, esterifying red rice and black levure are compounded according to proportion by weight ratio is 1:0.5-1:0.03-0.07, mixing uniformly; getting saccharifying levure of increasing incense which is used for shoyu; b). preparation of saccharifying levure of increasing incense which is used for shoyu product: it is composed of fermental red rice and esterifying red rice, the above-mentioned fermental red rice, esterifying red rice and are compounded according to proportion by weight ratio is 1:0.3-0.7, mixing uniformly; getting saccharifying levure of increasing incense which is used for shoyu product.

The invention provides an automatic oxygen increasing type constructed wetland device adopting hollow bricks, and relates to a constructed wetland system device used for treating wastewater. The automatic oxygen increasing type constructed wetland device comprises a water distributing pipe, wetland filling substrates (sandy soil, furnace slag and gravel), the shale hollow bricks, a water collecting tank and a water outlet, wherein the shale hollow bricks in the wetland system are placed transversely; no bonding substance is filled between the hollow bricks; and the hollow brick layer is gradually concave downwards at an angle of 20 DEG to 30 DEG, so that the wastewater infiltrates downwards and cannot fill the hollow bricks to reduce the oxygen supply capability. The hollow bricks on the two sides of the wetland are communicated with atmosphere, so that oxygen can be supplied conveniently and automatically; and meanwhile, the particle sizes and the filling thicknesses of the filling substrates are also screened to find out the best particle sizes and the best filling thicknesses of the substrates, so that the adsorption ability of the wetland on phosphorus in the wastewater is improved and the purification ability of the wetland system is enhanced. In summary, the automatic oxygen increasing type constructed wetland device has a simple and reasonable structure and achieves an ideal effect on eutrophic water body treatment.

The invention discloses an agricultural-piscatorial comprehensive planting-breeding method used for saline and alkaline land and relates to a saline and alkaline land improving method, in particular to adopted agricultural-piscatorial comprehensive planting-breeding saline and alkaline land and aims to solve the problem that an existing saline and alkaline land improving method is low in improvingefficiency, large in construction scale, high in investment, operation and maintenance cost and poor in improvement effect. The agricultural-piscatorial comprehensive planting-breeding method used for saline and alkaline land comprises the steps of saline and alkaline land layout, soaking and flushing, breeding pond preparation, fingerling stocking, breeding management including feeding putting,water oxygen content control, water quality regulation and control, periodical adding of bacillus powder towards breeding ponds, and soaking and flushing of a plurality of plot to be treated by utilizing pond breeding water in the breeding ponds. The agricultural-piscatorial comprehensive planting-breeding method is used for improving the saline and alkaline land.

The invention provides a preparation method of a compound photosynthetic bacterial fertilizer, and particularly provides a preparation method of the compound photosynthetic bacterial fertilizer obtained after mixed culture of thiocapsa roseopersicina and rhodopseudomonas sphaeroides which are high in growth speed, high in thallus content and short in culture time. A thiocapsa roseopersicina strain of purple sulfur photosynthetic bacteria and a rhodopseudomonas sphaeroides strain of purple non-sulfur photosynthetic bacteria are subjected to culture steps such as seed activation culture, seed culture, illumination culture in an anaerobic fermentation tank and the like, and the compound photosynthetic bacterial fertilizer containing thiocapsa roseopersicina and rhodopseudomonas sphaeroides is obtained. The compound photosynthetic bacterial fertilizer can realize disease prevention, fertilization, medicinal residue degradation and soil remediation, takes effect fast and has a stable effect, and the use amount is small.

The invention provides a preparation method of photosynthetic bacterial manure, and particularly provides the preparation method of photosynthetic bacterial manure for preventing and controlling hydrogen sulfide poisoning of rice. According to the preparation method, a thiocapsa roseoppersicina strain in purple sulfur photosynthetic bacterium is adopted and treated by steps of semi-solid seed activated culture, seed culture, illumination culture in an anaerobic fermentation tank and the like to obtain high-concentration bacterium liquid of the thiocapsa roseoppersicina; the high-concentration bacterium liquid can be capable of rapidly degrading hydrogen sulfide in a rice field, promoting root development of the rice and restoring soil so as to play an role in increasing the yield. According to the preparation method disclosed by the invention, the prescription of a culture medium for culturing the thiocapsa roseoppersicina and the culture condition are optimized a lot of times, so that the culture time is greatly shortened, 2 thousand of million cfu / ml of photosynthetic bacterium thiocapsa roseoppersicina bacterial manure can be obtained just after 7-10 days of fermentation, the production efficiency is improved and the production cost is lowered. In the photosynthetic bacterial manure disclosed by the invention, the thiocapsa roseoppersicina is high in hydrogen sulfide degrading speed and low in used amount for a large field; the effect of increasing the yield can be achieved by applying 100ml of the photosynthetic bacterial manure per mu every time.

The invention discloses a cadmium-contaminated soil modifier and a preparation method thereof and an improvement method for the cadmium-contaminated soil. The cadmium-contaminated soil modifier is prepared from the following raw materials in percentage by weight: 1 to 7 percent of polyaluminum chloride, 3 to 28 percent of ferric sulfate, 7 to 28 percent of calcium hydrogen phosphate, 35 to 85 percent of citric acid, 2 to 15 percent of polyacrylamide and 0.5 to 8 percent of L-proline. The invention also discloses the preparation method of the cadmium-contaminated soil modifier and the improvement method for the cadmium-contaminated soil. According to the cadmium-contaminated soil modifier and the preparation method thereof and the improvement method for the cadmium-contaminated soil, disclosed by the invention, the prepared modifier has the characteristics of surface adsorption, surface complexation and the like; the migration and effectiveness of cadmium in heavy metal contaminated soil can be significantly reduced, the absorption of the cadmium by plants is reduced, the physiological resistance of crops is improved, structure and properties of the soil are improved and the uniformity of soil microbes is improved; the cadmium-contaminated soil modifier is especially suitable for calcareous soil. The modifier has the advantages of easiness in raw material obtaining, low cost andsimple preparation; the improvement method for the cadmium-contaminated soil is simple and stable in effect.

The invention discloses a treatment method for reclamation soil fertility improvement of a coal mining subsidence area. The treatment method comprises the following steps: processing biomass charcoalgenerated by thermal cracking carbonization of straw in a certain degree, according to 1%-2% of an application amount, uniformly spreading on the surface of soil with an inorganic fertilizer mixture,and adequately mixing with the soil. The treatment method has the effects of improving a reclamation area soil structure, increasing soil fertility and remarkably increasing the yield of wheat crops.Through the combined application of the inorganic fertilizer and the biomass charcoal, organic carbon, total nitrogen, available phosphorus and available potassium ingredients of the soil can be remarkably improved, the recycling utilization of farmland crop straw can be realized, technical problems of improving the reclamation soil fertility and the crop yield can be solved, and the treatment method is provided for the soil fertility improvement of the coal mining subsidence area in China.

The invention discloses a method for realizing ecological cycle by combining planting and breeding in a sugarcane field. The method comprises the following steps: interplanting corns in the sugarcanefield at the early growth stage of sugarcane, processing fresh silage corns and sugarcane tail tips into a pig feed, and preparing biochar from withered straws; building a hog house and a chicken house beside the sugarcane field for raising pigs and chickens, wherein a cleaning waste liquid and pig urine of the hog house and the chicken house are used for drip irrigation of the sugarcane field, and excrement of the pigs and the chickens is used for raising hermetia illucens; feeding the chickens with produced hermetia illucens, after the chickens are hatched, breeding the chickens in the chicken house in a captive mode to a sugarcane elongation stage, and stocking the chickens to the sugarcane field; and preparing the hermetia illucens treated excrement and the biochar into a bio-organic fertilizer, and applying the bio-organic fertilizer to the sugarcane field so as to provide nutritional ingredients for growth of the sugarcane and the silage corns. By using of the method provided bythe invention, the sugarcane and the corns are planted; the pigs, chickens and hermetia illucens are bred; low input and high output of the sugarcane field can be achieved; and the ecological conceptof cyclic utilization is met.

The invention relates to a blast furnace hot blast stove coal gas system high-efficiency coal gas dispelling method. The blast furnace hot blast stove coal gas system high-efficiency coal gas dispelling method comprises the steps of connecting a three-way pipe to an outlet valve of a coal gas preheater before damping down of a blast furnace, connecting one end of the three-way pipe with an original coal gas drainer, connecting one end of the three-way pipe with a nitrogen hose, and communicating a port of the nitrogen hose with a nitrogen pipeline at a coke oven gas blending combustion valve;fully turning on a nitrogen purging valve to conduct purging on a coal gas pipeline after damping down of the blast furnace and burnout of a hot blast stove; turning off a front ball valve of a crudegas preheater drainer and turning on a nitrogen valve after no accumulated water continuously flows out from the coal gas preheater drainer, and introducing nitrogen into the colas gas preheater; andturning on the nitrogen valve in the position again after closing a lower blind plate of the coke oven gas blending combustion valve and introducing nitrogen into a coke oven gas blending combustion pipeline and a mixed coal gas main. Through the blast furnace hot blast stove coal gas system high-efficiency coal gas dispelling method, the total volume of nitrogen introduced into a coal gas systemis increased, effective replacement of nitrogen and coal gas in the pipelines is accelerated, and the purging time is substantially shortened.

The invention relates to an EM (Effective Microorganisms) tree nutrient solution. The EM tree nutrient solution comprises a nutrient bag A and a nutrient bag B. A preparation method of the EM tree nutrient solution is characterized by comprising the following steps: dissolving 1 part of the nutrient bag A and 0.1 part of the nutrient bag B in 1000 parts of pure water, stirring and dissolving uniformly to prepare the nutrient solution. A using method of the EM tree nutrient solution comprises the steps of irrigating the nutrient solution into a trickle irrigation nutrient bag, hanging the trickle irrigation nutrient bag on a transplanted tree for trickle irrigation or directly irrigating the nutrient solution into the root. Through the formula of the EM tree nutrient solution, the defect of the existing tree nutrient solution is solved.

The invention relates to the field of agricultural fertilizers, and specifically relates to a special organic and inorganic compound fertilizer for wheat. The special organic and inorganic compound fertilizer for wheat comprises an organic and inorganic compound material and a microorganism compound fungicide, and the organic and inorganic compound material comprises the following raw materials inpercentages by weight: 30% to 45% of urea, 18% to 30% of monoammonium phosphate, 3% to 10% of potassium chloride, 10% to 20% of organic matters, 5% to 10% of calcium sulfate, 2% to 6% of an adhesive,0.1% to 0.5% of an enveloping agent and 0.2% to 1% of an anti-blocking agent. According to the special organic and inorganic compound fertilizer for wheat, the content of chemical fertilizer is reduced, in addition, the organic matters and the microorganism compound fungicide are added, so that the supply for major elements P2O5 and K2O is guaranteed, the utilization of the organic matters and the activity of the microorganism compound fungicide are also guaranteed, the soil is improved, the soil fertility is fertilized, the using amount of chemical fertilizer is also reduced, and the purposeof improving the yield and quality of wheat is realized.

The invention belongs to the technical field of agricultural fertilization, and particularly discloses a fast fertilization method for newly-regulated soil, which includes the following steps: (1) optimizing the regulated soil; (2) organic fertilization; (3) biological fertilization: laying out earthworms according to 40-45kg / mu in each area after the step (2), watering 130-150kg / mu after stocking, covering rice straws with a thickness of 8-10cm, standing for 10-15 days, and carrying out biological fertilization by using the earthworms; (4) rotation and fertilization: adopting a sorghum and broad bean rotation mode in each area after step (3), and applying a compound fertilizer and a microbial fertilizer with strong soil fertility during sorghum planting; and (5) straw crushing and returning to the field: crushing the straws and returning to the field in each area in the step (4), and re-promoting the improved soil organic matters. The method is mainly used for agricultural fertilization and solves the problems of poor soil configuration, large difference in physical properties, non-uniform nutrients and unhealthy risks of soil quality in cultivated land after soil replacement in newly-regulated areas in the prior art.

The invention provides a preparation method of a microbial agent JF for preventing and treating corn stalk rot. Characterized in that it is characterized in that it comprises, the method comprises thefollowing steps: 1, preparation of 1S microbial agent powder; the preparation method comprises the following steps: adding activated bacillus sonorensis B-2, 3, 4-thiadiazole into a fermentation tank; the bacillus licheniformis strain is prepared from bacillus licheniformis B-26, bacillus licheniformis B-26, bacillus licheniformis B-26, bacillus licheniformis B-26, bacillus licheniformis B-26 andbacillus licheniformis B-26. The preparation method comprises the following steps: respectively carrying out seed culture and fermentation culture on single colonies, namely bacillus solani N-16 andbacillus pumilus N-17, and adsorbing the four cultured bacterial solutions by using an adsorbent, so as to obtain microbial agent powder; mixing the four microbial agent powders according to a mass ratio of 1: 1: 1: 1 to obtain a mixed powder; and 2, preparation of a 2S microbial agent JF: mixing the microbial agent powder prepared in step 1 with auxiliary materials to prepare the microbial agentJF. The microbial agent JF prepared by the invention can effectively improve saline-alkali soil and prevent corn stalk rot of the saline-alkali soil.

The invention provides an organic fertilizer. The organic fertilizer comprises, by mass, 1-5parts of potassium carbonate, 1-10 parts of humic acid, 1-5 parts of chlorobromoisocy anuric acid and 20-70 parts of Derris trifoliata Lour. stem leaf dry powder. The organic fertilizer has desinsection and disinfection effects, can substantially improve the fertility of soil, and greatly increases the output of crops.

The invention discloses an improvement method for acid red soil in dry land, improved red soil and application of the improved red soil. The improvement method comprises the following steps that mud cakes obtained after river sediments are subjected to dehydration treatment are mixed with biomass charcoal, and then the obtained mixture is applied to the acid red soil; and the acid red soil is subjected to manual or mechanical ploughing, so evenly-mixed mixed soil is obtained. Alternatively, the method comprises the following steps: respectively applying the mud cakes obtained by dehydrating the river sediments and the biomass charcoal to the acid red soil; and manually or mechanically ploughing the acid red soil so as to obtain the mixed soil which is evenly mixed. According to the improvement method, recyclable components in solid waste are utilized; the improvement method is friendly to environment, simple in process, low in cost and suitable for large-area application; the contents of total nitrogen and available phosphorus in the acid red soil of dry land can be increased; the pH value of the soil is increased; the physicochemical property of the dry land acid red soil and the growth condition of crops are effectively improved; and the improved red soil has a growth promoting effect on various crops.

The invention discloses multifunctional organic pesticide. The multifunctional organic pesticide comprises, by weight, 20-30 parts of bamboo vinegar, 1-3 parts of allicin oil, 9-11 parts of rotenone, 3-10 parts of auricularia auricular extract, 2-5 parts of defoamer, 3-5 parts of a disintegrating agent, 0.5-1 part of antioxygen and 40-50 parts of water. The multifunctional organic pesticide has the advantages that the bamboo vinegar, the allicin oil, the rotenone and the auricularia auricular extract are adopted, raw materials are easy to obtain, good permeability and absorbability of plants on tissue are achieved, application effect of the pesticide and fertilizer can be improved, application dosage can be reduced, and meanwhile, total nitrogen and available phosphorus content in soil can be increased with the bamboo vinegar.

The invention relates to a complex microbial inoculant which comprises the following components in parts by weight: 40-50 parts of nitrifying bacteria, 10-12 parts of denitrifying bacillus, 1-5 partsof colorless sulfur bacteria, 3-5 parts of photosynthetic bacteria, 3-8 parts of anaerobic bacteria, 3-8 parts of facultative bacteria, 3-8 parts of aerobic bacteria, 4-8 parts of a biological enzyme,and 45-60 parts of a nutritional agent. According to the complex microbial inoculant disclosed by the invention, multiple strains are composed of decomposable bacteria, synthetic bacteria, anaerobicbacteria, facultative bacteria and aerobic bacteria, a coexisting biological system is composed of the complex microbial inoculant and the multiple strains by adding a proper amount of biological enzymes and nutritional agents, functions are exerted synergistically according to mutual survival, and the complex microbial inoculant can be directly added into wastewater during use; activated sludge,flour, urea and other substances are not needed, and time and labor are saved.

The invention belongs to the technical field of sewage treatment and particularly relates to a reinforcing system for reducing nitrate nitrogen concentration in sewage. The reinforcing system consists of a reaction tank and an aquatic plant recovery tank and is characterized in that the reaction tank consists of a contact area and a reaction area; the aquatic plant recovery tank consists of a recovery tank front end and a recovery tank rear end; and the reaction tank and the aquatic plant recovery tank, the contact area and the reaction area and the recovery tank front end and the recovery tank rear end are all spaced by a bank respectively. In the invention, by adding a carbon source into sewage, the removal rate of total nitrogen and total phosphorus in sewage is increased; and moreover, the design is reasonable and skillful, the cost is low, the raw materials are easily available, and environmental pollution is avoided. The system provided by the invention realizes a good treatment effect on the nitrate nitrogen in sewage and can reduce the nitrate nitrogen concentration in sewage to the greatest degree.

The invention discloses a cultivation method for interplanting fructus lycii and medicago sativa. The cultivation method for interplanting the fructus lycii and the medicago sativa comprises the steps that the medicago sativa is interplanted among fructus lycii rows for field planting, the medicago sativa is cut for 2-5 times every year, the harvested medicago sativa is smashed, turned and pressed into green manure for field application, the medicago sativa is cut for the last time in a third interplanting year, roots of the medicago sativa is broken, turned and pressed, and the medicago sativa is interplanted again in the next year. According to the cultivation method for interplanting the fructus lycii and the medicago sativa, the fructus lycii and the medicago sativa are interplanted, so that the soil nutrient is improved, the soil fertility is improved, the use amount of chemical fertilizer is reduced, and meanwhile, the yield and quality of the fructus lycii are effectively improved.

The invention discloses a tobacco NtMLO6-1 gene as well as a knockout method and application thereof. The base sequence of the tobacco NtMLO6-1 gene is as shown in SEQ ID No. 1. The method comprises the following steps: knocking out a gene editing plant of an NtMLO6-1 gene by utilizing a CRISPR / Cas9 mediated gene editing technology; the total nitrogen content and the amino acid content in the NtMLO6-1 gene knockout edited plant leaves are remarkably increased, and genetic materials and references are provided for tobacco MLO gene family regulation mechanism research and tobacco leaf total nitrogen and amino acid content regulation.

The invention discloses a leuciscus waleckii-pasture comprehensive planting and breeding saline-alkali soil improving method, and relates to a saline-alkali soil improving method, in particular to a method adopting leuciscus waleckii-pasture comprehensive planting and breeding for saline-alkali soil improving. The problems that according to existing saline-alkali soil improving methods, the improving efficiency is low, the project scale is large, the investment and operation cost is high, and the improvement effect is poor are solved. The leuciscus waleckii-pasture comprehensive planting and breeding saline-alkali soil improving method comprises the steps of 1 laying out the saline-alkali soil; 2, performing soaking and flushing; 3, preparing a culture pond; 4, performing fingerling stock;5, performing culture management, wherein feed feeding is performed, the oxygen content in water is controlled, the water quality is regulated, and bacillus powder is added into the culture pond periodically; 6, utilizing pond culture water in the culture pond for performing soaking and flushing in a plurality of land parcels to be treated. The method is used for saline-alkali soil improvement.