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Method for preparing protein feed by using Jerusalem artichoke residues

A protein feed and slag technology, applied in animal feed, animal feed, application, etc., to reduce the discharge of waste water, reduce waste water discharge, and improve the value of feed

Inactive Publication Date: 2012-06-27
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems existing in the recycling of waste residues and distiller's grains filtrate in the prior art, the present invention provides a method for producing protein feed by fermenting Jerusalem artichoke residues, which not only solves the problems of waste disposal and resource utilization, but also Improve the feed value of Jerusalem artichoke lees, and have very important significance to environmental protection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for producing protein feed by fermenting Jerusalem artichoke dregs, the specific steps are as follows:

[0033] 1) Preparation of raw materials and cultivation of strains:

[0034] The preparation method of Jerusalem artichoke residue and supernatant concentrate is as follows: take the residue after distilling and extracting ethanol, and centrifuge it for 10 minutes under the condition of 4500r / min, and obtain the precipitate which is Jerusalem artichoke residue; the supernatant obtained in the preparation process Heat and concentrate to make the sugar content reach more than 30%, which is the supernatant concentrate;

[0035]Prepare nutrient salt solution, its composition is: MgSO 4 ·7H 2 O 30g / L, MnSO 4 ·H 2 O 0.6g / L, FeSO 4 ·7H 2 O 0.16g / L, ZnSO 4 ·7H 2 O 0.5g / L and CaCl 2 0.7g / L;

[0036] The preparation process of Kluyveromyces marxii seed liquid is as follows: take Kluyveromyces marx, activate it on a slant medium for 48-72 hours, and un...

Embodiment 2

[0051] A method for producing protein feed by fermenting Jerusalem artichoke dregs, the specific steps are as follows:

[0052] 1) Preparation of raw materials and cultivation of strains:

[0053] The preparation of raw material and the cultivation of bacterial classification are identical with embodiment 1;

[0054] 2) Fermentation culture:

[0055] Fresh dregs and bran are mixed in a ratio of 8.75: 1.25 by weight, then 1% urea and 0.5% potassium dihydrogen phosphate are added, and 5 mL of supernatant concentrate, nutrient Add 5mL of salt solution and 7.5mL of distilled water into the supernatant concentrate, nutrient salt solution and distilled water, stir and mix well, then adjust the pH value to 5.5 with 0.1mol / L hydrochloric acid or sodium hydroxide solution, and put it into an autoclave Inside, sterilize at 121°C for 30 minutes, inoculate the mixed bacterial solution after cooling, the inoculation amount is 5%, mix well with sterilized glass rods, and cultivate at a co...

Embodiment 3

[0059] A method for producing protein feed by fermenting Jerusalem artichoke dregs, the specific steps are as follows:

[0060] 1) Preparation of raw materials and cultivation of strains:

[0061] The preparation of raw material and the cultivation of bacterial classification are identical with embodiment 1;

[0062] 2) Fermentation culture:

[0063] Mix the fresh dregs and bran at a weight ratio of 8.5:1.5, then add 1% urea and 0.5% potassium dihydrogen phosphate, and then add 5mL of the supernatant concentrate, nutrient Add the supernatant concentrate, nutrient salt solution and distilled water in the ratio of 5mL of salt solution and 10mL of distilled water, stir and mix well, then adjust the pH value to 5.5 with 0.1mol / L hydrochloric acid or sodium hydroxide solution, and put it in an autoclave , sterilize at 121°C for 30 minutes, inoculate the mixed bacterial solution after cooling, the inoculation amount is 5%, mix well with sterilized glass rods, and cultivate at a co...

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PUM

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Abstract

The invention provides a method for preparing a protein feed by using Jerusalem artichoke residues. The method is as follows: after Jerusalem artichoke is fermented by alcohol yeasts for production of ethanol, distillation is carried out, then solid-liquid separation is carried out with a centrifuge on a residue solution obtained after distillation so as to obtain the Jerusalem artichoke residues, and clear liquid obtained after centrifugation is condensed through heating so as to allow total sugar content in the clear liquid to be more than 30%; the residues, clear liquid concentrate and wheat bran are mixed, urea is added, a mixed strain of Kluyveromyces marxianus, Geotrichum candidum and Candida utilis is inoculated, an obtained mixture is cultured at a temperature of 30 DEG C for 5 d and maintained at a constant temperature of 80 DEG C after culture until the mixture is dried, and the dried mixture is crushed so as to prepare the Jerusalem artichoke residue solution protein feed. According to the invention, the protein feed is produced through fermentation of the Jerusalem artichoke residues; therefore, the problems of treatment and resource utilization of waste produced in preparation of the fuel ethanol from the raw material Jerusalem artichoke are overcome, discharge of waste produced in preparation of the fuel ethanol from the raw material Jerusalem artichoke is reduced, pollution to environment is mitigated, and the value of the Jerusalem artichoke residue solution in the aspect of feeds is improved.

Description

technical field [0001] The invention relates to the recovery and utilization of waste dregs after ethanol production from Jerusalem artichoke, in particular to a method for producing protein feed by fermenting jerusalem dregs. Background technique [0002] Facing the dwindling oil resources, the aggravating energy crisis and the resulting environmental pollution, countries all over the world are seeking to replace fossil energy with renewable and clean energy. Fuel ethanol produced by fermentation from biomass is a renewable and clean energy source. In my country, fuel ethanol is mainly produced from corn. However, since corn ethanol uses grain as raw material, it must occupy a large amount of cultivated land, which is in conflict with national food security. The premise is to cultivate land, not consume food and not destroy the ecological environment. [0003] In this regard, Jerusalem artichoke has unique advantages: first, Jerusalem artichoke has strong stress resistance...

Claims

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Application Information

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IPC IPC(8): A23K1/06A23K10/38
CPCY02P60/87
Inventor 汪伦记纠敏张勇法尤晓岩李净净
Owner HENAN UNIV OF SCI & TECH
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