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Kluyveromyces and application thereof in production of xylitol

A Kluyveromyces, xylitol technology, applied in the field of microorganisms, can solve the problems of large environmental pollution and high requirements for chemical equipment

Active Publication Date: 2021-03-19
苏州聚维元创生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, microbial fermentation still uses xylose produced by acidification of corncobs as raw material, and the xylose preparation process has great environmental pollution and high requirements on chemical equipment

Method used

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  • Kluyveromyces and application thereof in production of xylitol
  • Kluyveromyces and application thereof in production of xylitol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] This example is used to illustrate the screening, purification and identification methods of Kluyveromyces.

[0048] The applicant collected decaying leaves of sweet potato plants in the intertidal zone of the mangrove area in Haikou, Hainan in May 2020, and used the following method to isolate 12 strains of Kluyveromyces, and screened 3 strains with high xylitol production.

[0049] (1) Preparation of separation medium

[0050] GYPS medium (g / L): 4 glucose, 4YE, 4 peptone, 14 agar, 25 sea salt. Sterilize at 115°C for 15 minutes. After the sterilization is completed, the plate is poured into the ultra-clean bench to obtain the Kluyveromyces isolation medium.

[0051] (2) Isolation, purification and identification of Kluyveromyces

[0052] Collected decayed leaves of mangrove plants shake off the silt on the leaves, wipe the scissors with 75% ethanol, cut them into 1cm square pieces, wash them with sterile seawater for 1-2 times, stick them flat on the GYPS plate, and...

Embodiment 2

[0069] In this example, the production of xylitol is described in detail by using the tail liquid of wheat straw straw sugar fermentation by Corynebacterium glutamicum as raw material. The straw sugar mentioned in the present invention is a mixture of glucose and xylose. Specific steps are as follows:

[0070] (1) Preparation of straw sugar

[0071]Preparation of wheat straw sugar: Wash and chop the wheat straw raw material, and pretreat the wheat straw by steam explosion. The wheat straw is then treated with sodium hydroxide and sodium sulfite, the weight of sodium hydroxide: dry weight of wheat straw raw material is 1:6, the weight ratio of sodium sulfite: sodium hydroxide is 1:8, and the treatment conditions are: the reaction temperature is 150°C , the time is 1h. After fully removing lignin, extract wheat straw fibers, dehydrate and fully wash away residual lye; soak straw fibers in acetic acid-sodium acetate (or similar buffer pair with buffering effect) buffer solution...

Embodiment 3

[0085] This example describes in detail the production of xylitol from the rice straw sugar fermentation tail liquid of chlorella. The straw sugar in the present invention is a mixture of glucose and xylose. Specific steps are as follows:

[0086] (1) Preparation of straw sugar:

[0087] Preparation of rice straw sugar: Wash and chop the raw material of rice straw, and pretreat the rice straw by steam explosion. The wheat straw is then treated with sodium hydroxide and sodium sulfite, the weight of sodium hydroxide: the absolute dry weight of rice straw raw material is 1:10, the weight ratio of sodium sulfite: sodium hydroxide is 1:5, and the treatment conditions are: the reaction temperature is 160°C , the time is 1h. After fully removing lignin, extract rice straw fibers, dehydrate and fully wash off residual lye; soak straw fibers in acetic acid-sodium acetate (or similar buffer pair with buffering effect) buffer solution, add cellulase, Hydrolyze the fiber pulp into a h...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to Kluyveromyces. The Kluyveromyces is Kluyveromyces siamensis THUZTY2041, the preservation date is October 19, 2020, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), the preservation address is institute of microbiology, Chinese Academy of Sciences, 3#, 1# yard, West Beichen road, Chaoyang District, Beijing, and the preservation number is CGMCC NO. 20917. The invention further provides application of the Kluyveromyces in production of xylitol. Straw sugar fermentation tail liquid is used as a raw material, the Kluyveromyces can be cultured at high temperature to obtain the xylitol under the condition of low cost, the condition constraint of traditional industrial production is eliminated, the advantages of high-temperature fermentation production are exerted, and the comprehensive utilization value of agricultural straw is improved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a kluyveromyces and its application in producing xylitol. Background technique [0002] Xylitol is a five-carbon sugar alcohol widely used in chemical, food, pharmaceutical, leather and feed industries. As a food additive, its sweetness is equivalent to that of sucrose, and it is non-cariogenic. Its metabolism in the body does not require the participation of insulin and will not cause an increase in blood sugar. It has important application value in diabetics and anti-caries foods. [0003] At present, the source of xylitol is mainly industrial production, which is catalyzed by hydrogenation of D-xylose under high temperature and high pressure. This method has high requirements for raw materials, high energy consumption, and complicated process, which limits the large-scale production of xylitol. At the same time, nickel needs to be added in the production pr...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/36C12N1/02C12P7/18C07C29/74C07C31/18C12R1/645
CPCC12N1/16C12N1/36C12N1/02C12P7/18C07C29/74C07C31/18
Inventor 张天元李振
Owner 苏州聚维元创生物科技有限公司
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