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Construction and application of high-temperature high-yield engineering strain for producing ethanol

A technology of engineering strains and ethanol, applied in the direction of fungi, microorganism-based methods, microorganisms, etc., can solve the problems of fermentation rate, yield and insufficient ability to utilize xylose

Active Publication Date: 2014-11-26
UNIV OF SCI & TECH OF CHINA
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Problems solved by technology

The above studies have not fully exploited the potential of thermotolerant yeast to ferment xylose to produce ethanol at high temperature, and their rate, yield and ability to utilize xylose fermentation are far from enough (Table 1)

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  • Construction and application of high-temperature high-yield engineering strain for producing ethanol
  • Construction and application of high-temperature high-yield engineering strain for producing ethanol
  • Construction and application of high-temperature high-yield engineering strain for producing ethanol

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Embodiment Construction

[0023] The technical solution of the present invention will be described in further detail below. It should be noted that the various embodiments of the present invention can be combined in any way as desired.

[0024] The first aspect of the present invention provides a heat-resistant engineering yeast strain that can efficiently utilize xylose for fermentation. In one embodiment, the bacterial strain is obtained by the following method: using the heat-resistant yeast K. marxianus strain with knockout of xylose reductase gene and xylitol dehydrogenase gene as host, and NcXYL1, PsXYL2-ARS, KmXYL3 was recombinantly expressed in the host.

[0025] In a preferred embodiment, the strain contains the xylitol reductase gene NcXYL1 of Neurospora crassa.

[0026] In a preferred embodiment, the mutant gene PsXYL2-ARS has a D207A / I208R / F209S mutation on the basis of the wild-type xylitol dehydrogenase gene.

[0027] In a preferred embodiment, the strain contains two copies of the mut...

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Abstract

The invention relates to construction and an application of a high-temperature high-yield engineering strain for producing ethanol; specifically, the xylose reductase gene derived from neurospora crassa, the xylitol dehydrogenase mutant gene of scheffersomyces stipitis, and a series of downstream genes are efficiently expressed or knocked out in heat-resistant kluyveromyces marxianus, so that the strain is capable of efficiently utilizing and fermenting xylose, and rapidly producing ethanol in large quantity at a high temperature (greater than 42 DEG C) through an xylose metabolism path. The yeast strain is capable of rapidly and efficiently fermenting high-concentration xylose at 42 DEG C for producing ethanol; and both of the yield and the fermentation speed exceed those of the reported heat-resistant strain.

Description

technical field [0001] The present invention relates to the field of biotechnology. In particular, it relates to the technical field of improving the production of ethanol by fermenting xylose under high temperature through the transformation of engineering strains. Specifically, the present invention constructs a heat-resistant engineering yeast strain capable of producing ethanol by fermenting xylose under high-temperature conditions and greatly increasing the ethanol production rate and yield. Background technique [0002] Xylose is the main product of the hydrolysis of hemicellulose biomass and is the second most abundant fermentation feedstock in the world (Zhang et al., 2011). As the most abundant five-carbon sugar in lignohemicellulose hydrolyzate, xylose is undoubtedly the key to produce high value-added products from renewable biomass (Sasaki et al., 2010). [0003] Ethanol is a kind of alcohol and the main component of wine, so it is also commonly known as alcoho...

Claims

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Application Information

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IPC IPC(8): C12N1/19C12P7/06C12R1/645
CPCY02E50/10
Inventor 洪泂张佳张标
Owner UNIV OF SCI & TECH OF CHINA
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