Kluyveromyces marxianus strain culture medium and strain culture method thereof

A cultivation method and yeast technology, applied in the field of microorganism cultivation, can solve the problems of uneven distribution of medium and waste of manpower, etc.

Active Publication Date: 2021-01-12
XINJIANG TIANRUN BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to make up for the deficiencies of the prior art and solve the problem of using the fed-batch method for high-density cultivation of Kluyveromyces marx strains in the prior art, it is necessary to manually add the medium, which is not only a wast

Method used

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  • Kluyveromyces marxianus strain culture medium and strain culture method thereof
  • Kluyveromyces marxianus strain culture medium and strain culture method thereof
  • Kluyveromyces marxianus strain culture medium and strain culture method thereof

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Embodiment approach

[0040] As an embodiment of the present invention, the sealing cover 41 is provided with a first groove 8 on the side close to the feeding box 4; a moving plate 81 is slidably connected in the first groove 8; the moving plate 81 is close to One side of the sealing cover 41 is fixedly connected with an auxiliary rod 82; the auxiliary rod 82 runs through the sealing cover 41; the end of the auxiliary rod 82 located at the outer side of the sealing cover 41 is fixedly connected with a gravity box 83; a gravity ball is placed in the gravity box 83 84; During work, since the process of inflating the booster chamber 52 into the fluid addition box 4 is intermittent, the air pressure in the fluid addition box 4 increases during inflation, and when the inflation is stopped, the airflow in the fluid addition box 4 passes through The third through-slot 7 gradually leaks out, thereby gradually reducing the air pressure in the feed tank 4. With the rotation of the motor 21, the airflow in th...

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Abstract

The invention belongs to the technical field of microorganism culture, and particularly relates to a kluyveromyces marxianus strain culture medium and a strain culture method thereof. The kluyveromyces marxianus strain culture medium comprises a base, a box body and a motor, wherein the box body is designed to be in a disc shape; the base is fixedly connected with the bottom of the box body; a culture cavity is formed in the box body; the motor is fixedly connected to one side of the box body; an output shaft of the motor penetrates through the box body and extends into the culture cavity; a rotating wheel is fixedly connected to one end positioned in the culture cavity, of the output shaft of the motor; and an annular groove is formed in one side close to the motor, of the culture cavity.By increasing air pressure in a feeding box, a liquid culture medium drips into the culture cavity through the micropores under the action of pressure and gravity and falls into culture dishes on bearing plates in the dripping process in the culture cavity, and liquid drops carry dissolved oxygen and nutrient substances to drip into the culture dishes. Nutrients and oxygen are provided for kluyveromyces marxianus strains in the culture dishes.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, in particular to a Kluyveromyces marx strain culture medium and a strain cultivation method thereof. Background technique [0002] In the prior art, by controlling the addition of carbon sources in batches, it is possible to effectively accelerate the breeding speed and enhance the activity of the strains during the cultivation of Kluyveromyces marxe strains. Add the carbon source in the dish, and then use stirring to make the carbon source evenly distributed in the culture dish. In the process of high-density culture, stirring can not make the added carbon source and the original medium well. Mixing, and the stirring effect can easily affect the normal reproduction of the strain, resulting in a decrease in the growth and reproduction rate of the strain. At the same time, the Kluyveromyces marx strain is an aerobic bacteria, and its growth and reproduction rate are in a low-spee...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12M1/00C12M1/22C12M1/12C12M1/04C12R1/645
CPCC12M23/02C12M23/10C12M23/38C12M23/48C12M29/04C12M29/06C12N1/16
Inventor 李新玲牟光庆罗晓红尤宏杨迎春李福赞张亚南安美玲李梦
Owner XINJIANG TIANRUN BIOTECH
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