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Microbial preparation prepared by fermentation of stalk hydrolysate and use thereof

A microbial agent and ethanol technology, applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, can solve the problems of increasing machinery, labor, energy consumption and cost, increasing production procedures, increasing process flow, etc., and achieve strong resistance to miscellaneous bacteria Pollution characteristics, save fermentation cost, and shorten the effect of process flow

Inactive Publication Date: 2009-03-11
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the point of view of practical operation, the concentration of hydrolyzate will increase the production process; if the fermentation requires higher sugar concentration, it will greatly increase the cost of producing ethanol from cellulosic materials
[0005] In addition, for the fermentation of hydrolyzate, it generally needs to go through lime (overliming) or sulfite detoxification process. While increasing the process flow, it increases machinery, labor, energy consumption and cost, and causes sugar loss at the same time.

Method used

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  • Microbial preparation prepared by fermentation of stalk hydrolysate and use thereof
  • Microbial preparation prepared by fermentation of stalk hydrolysate and use thereof
  • Microbial preparation prepared by fermentation of stalk hydrolysate and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, isolation and identification of bacterial strain

[0020] 1. Isolation of strains

[0021] In September 2006, five orchards near Xiangshan, Haidian District were selected, and surface soil samples within the projection range of the canopy under the fruit trees were collected respectively, and put into clean plastic bags for retrieval.

[0022] Selective culture was carried out with dilute acid hydrolyzate of straw as substrate to enrich ethanol fermenting bacteria. The dilute acid hydrolyzate of straw is obtained as follows: corn straw is crushed through a 20-mesh sieve, 1% by volume of dilute sulfuric acid is added, and the solid content of 10% by mass is hydrolyzed at 110° C. for 40 minutes. Weigh 5g of the recovered soil samples, add the prepared dilute acid hydrolyzate in an amount of 5% by mass, group them according to the numbers of different sampling points, and then cultivate them. The culture temperature is 34°C, the rotation speed is 70r / min, a...

Embodiment 2

[0037] Embodiment 2, the preparation of microbial bacterial agent

[0038] Trichoderma konigii Oudem ACCC 30167 was inoculated in potato dextrose agar liquid medium at 150r / min, and activated at 30°C for 10h. Yeast (Trichosporon aquatic) M04 CGMCC2443 was inoculated in liquid YEPD medium at 150r / min, and activated at 30°C for 24h. Kluyveromyces (Saccharomyces kluyveri) M02 CGMCC 2442 was inoculated in liquid YEPD medium at 150r / min, and activated at 30°C for 24h. Trichoderma konigii Oudem (Trichoderma konigii Oudem) ACCC 30167 bacterium liquid, saccharomycete (Trichosporon aquatile) M04 CGMCC 2443 bacterium liquid and Kluyveromyces (Saccharomyces kluyveri) M02 CGMCC 2442 bacterium liquid are mixed to prepare microbial inoculum, The colony-forming unit ratio of Trichoderma konigii Oudem ACCC 30167, Trichosporonaquatile M04 CGMCC 2443 and Saccharomyces kluyveri M02 CGMCC2442 in the agent was 1:1:1.

Embodiment 3

[0039] Embodiment 3, the preparation of microbial bacterial agent

[0040] Trichoderma konigii Oudem ACCC 30167 was inoculated in potato dextrose agar liquid medium at 150r / min, and activated at 30°C for 18h. Yeast (Trichosporon aquatic) M04 CGMCC2443 was inoculated in liquid YEPD medium at 150r / min, and activated at 30°C for 24h. Kluyveromyces (Saccharomyces kluyveri) M02 CGMCC 2442 was inoculated in liquid YEPD medium at 150r / min, and activated at 30°C for 24h. Trichoderma konigiiOudem (Trichoderma konigiiOudem) ACCC 30167 bacterium liquid, saccharomycete (Trichosporon aquatile) M04 CGMCC 2443 bacterium liquid and Kluyveromyces (Saccharomyces kluyveri) M02 CGMCC 2442 bacterium liquid are mixed to prepare microbial inoculum. The colony forming unit ratio of Trichoderma konigii Oudem ACCC30167, Trichosporonaquatile M04 CGMCC 2443 and Saccharomyces kluyveri M02 CGMCC2442 in the bacterial agent was 1:2:2.

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Abstract

The invention discloses a microbial inoculum for manufacturing ethanol by fermentation of straw hydrolyzate and application thereof. The microbial inoculum disclosed by the invention is inoculum using Trichoderma konigii Oudem, Trichosporon aquatile and Saccharomyces kluyveri as active ingredients. The invention also discloses a method for producing ethanol, which is to use the microbial inoculum to ferment the straw hydrolyzate to produce the ethanol. Ethanol is the main metabolite generated by fermentation of the microbial inoculum by using the straw hydrolyzate as substrate, and accounts for higher proportion reaching 12.19 gram ethanol / liter fermented solution, thereby creating excellent precondition for further extraction and purification of ethanol.

Description

technical field [0001] The invention relates to a microbial agent for producing ethanol by fermenting straw hydrolyzate and its application. Background technique [0002] In the production of ethanol by simultaneous saccharification and fermentation (Simultaneous Saccharification and Fermentation (SSF) method), the biggest bottleneck lies in the difference between the enzymatic hydrolysis temperature and the ethanol fermentation temperature. Secondly, in the SSF method, live fungi are used to hydrolyze cellulose, and the fungi require a higher oxygen concentration, which will affect the normal function of the ethanol fermentation strain, because the sugar generated by enzymatic hydrolysis is mainly used for cell growth Proliferate with quantity, rather than ferment to produce ethanol. Again, the ethanol fermentation strains in the SSF method are sensitive to the inhibitory factors in the fermentation broth. Due to the above-mentioned defects in the existing ethanol ferment...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/14C12N1/16C12P7/10C12R1/885C12R1/645C12R1/85
CPCY02E50/16Y02E50/10
Inventor 李国学路鹏沈世华任丽梅江滔
Owner CHINA AGRI UNIV
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