Method for preparing lactose-free dairy product by using immobilized lactase

Abstract

The invention discloses a method for preparing lactose-free dairy products by using immobilized lactase, and belongs to the technical field of lactose-free dairy product preparation. In the present invention, the crude enzyme liquid is prepared by fermenting and culturing Kluyveromyces brittleness, the purified lactase is obtained through salting out and desalting, and the purified lactase is cross-linked and fixed on the attapulgite with glutaraldehyde to obtain the immobilized lactase , and then use it in the enzymatic hydrolysis of raw milk, so as to obtain a method for preparing lactose-free dairy products by using immobilized lactase. Examples prove that the present invention is easy to operate, not only improves the stability of the enzyme by using immobilized lactase, reduces the content of lactose in fermented milk, and reaches the lactose-free standard stipulated by the state, but also produces lactose-free fermented milk products with excellent taste Good, rich in nutrition, easily absorbed by the human body, suitable for large-scale production and application.

Description

technical field [0001] The invention discloses a method for preparing lactose-free dairy products by using immobilized lactase, and belongs to the technical field of lactose-free dairy product preparation. Background technique [0002] Lactose-free refers to the use of a certain process (lactase) to remove the lactose in the milk. People with too little lactase secretion should eat lactose-free milk products. [0003] Lactose is a unique disaccharide in animal milk. It cannot be directly absorbed by the human body. It must be hydrolyzed into galactose and glucose by lactase. However, because Asians do not have the habit of drinking milk on a daily basis, a large part of the population lacks lactase in their bodies. Drinking milk After the typical performance is diarrhea, that is, lactose intolerance. In addition, during the fermentation process of fermented milk, although part of the lactose is decomposed, there is still a certain amount of lactose residue. People with seve...

AI Technical Summary

Problems solved by technology

[0006] The technical problem mainly solved by the present invention: in the process of preparing lactose-free dairy products, free lactase is often used to enzymatically hydrolyze raw milk, resulting in reduced enzyme activity or even failure in the fermentation stage, and greatly reduced enzymatic hydrolysis efficiency , even if the use of lactase is increased, the current situation of lactose-free fermented milk limited by the national standard cannot be produced. A crude enzyme solution obtained by fermentation and cultivation of Kluyveromyces fragilis is provided, which is obtained by salting out and desalting Purify lactase, cross-link it with glutaraldehyde and immobilize it on attapulgite to obtain immobilized lactase, and then use it in the enzymatic hydrolysis of raw milk to obtain lactose-free dairy products using immobilized lactase Methods