Sunflower seed meal microbial protein feed and preparation method thereof
A technology of microbial protein and sunflower seed meal, applied in animal feed, animal feed, application, etc., can solve the problems of high crude fiber content, indigestible macromolecular protein, restrictions on the use of sunflower meal, etc., to improve immunity, improve Disease resistance and stress resistance, the effect of reducing livestock morbidity
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Embodiment 1
[0048] 1. Preparation of solid-state fermentation medium:
[0049] Weigh 80 parts by weight of sunflower seed meal and 20 parts by weight of wheat bran, add water and stir evenly to obtain a solid-state fermentation medium, control the water content of the solid-state fermentation medium to be 40-60%, and the sunflower seed meal can be The shelled sunflower seed meal can also be shelled sunflower seed meal, and the effect of using the shelled sunflower seed meal will be better. What adopted in this embodiment is shelled sunflower seed meal, which is provided by Haixin Oil Press Factory of Mongolian Autonomous County, Fuxin City. The wheat bran is commercially available.
[0050] 2. Preparation of mixed strains:
[0051] 1. Preparation of Kluyveromyces marx seed solution:
[0052]① Preparation of Kluyveromyces marx slant strains:
[0053] Kluyveromyces was provided by Jiangsu Institute of Microbiology, and the slant medium was YPG glucose agar medium. Prepare K. marx slant...
Embodiment 2
[0078] In this example:
[0079] When preparing the solid-state fermentation medium, control the water content of the solid-state fermentation medium to be 50%.
[0080] When preparing the Kluyveromyces maxima seed liquid, the Kluyveromyces maximus used was provided by the Jiangsu Institute of Microbiology, and Kluyveromyces maxima is a lysine-accumulating mutant strain obtained through AEC mutagenesis.
[0081] The mixed strains were sprayed on the solid-state fermentation medium, and the anaerobic fermentation was sealed for 48 hours, and the temperature was controlled at 30°C. Carry out aerobic fermentation 48 hours then, control temperature is 30 ℃. All the other are with embodiment 1.
[0082] The composition of the sunflower meal protein feed prepared by the method of the present invention is shown in Table 1
[0083] Table I
[0084]
[0085] The effect of the present invention is illustrated by experiment below:
[0086] In the present invention, the acid-base ...
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