39results about How to "Strong production" patented technology

The invention relates in part to plant HMG2 HMGR genes and in part to the "post-harvest" production method of producing gene product of interest in plant tissues and cultures. The HMG2 promoter elements are responsive to pathogen-infection, pest-infestation, wounding, or elicitor or chemical treatments. The HMG2 elements are also active in specialized tissues of the plant including pollen and mature fruits. HMG2 promoter elements and HMG2-derived promoters can be advantageously used to drive the expression of disease and pest resistance genes, whereby transgenic plants containing such gene constructs would be resistant to the targeted disease and pest. In particular, the HMG2 gene expression system can be utilized in developing nematode resistant plants. The post-harvest production method of the invention utilizes plant tissues and cell cultures of plants or plant cells engineered with a expression construct comprising an inducible promoter, such as the HMG2 promoter, operably linked to a gene of interest. Production of the desired gene product is obtained by harvesting, followed by inducing and processing the harvested tissue or culture. The post-harvest production method may be advantageously used to produce direct or indirect gene products that are labile, volatile, toxic, hazardous, etc.

The invention pertains to the use of (i) one or more C2-C6 alpha-hydroxy acids, salts of these acids, esters of these acids and combinations thereof; and (ii) one or more compounds selected from—the group of amino acid derivatives consisting of γ-glutamyl amino acids, pyroglutamyl amino acids, glutamate-containing or proline-containing dipeptides, oxo-aminoacids, homo-amino acids, N-acetyl amino-acids and glycyl-glycine; -phenolic acid derivatives; -linear C2-C6 or cyclic C6 sugar alcohols; -pyridinic acid derivatives; and -nucleobases and / or nucleotides chosen from uracil, adenine, adenosine 3′-monophosphate (3′-AMP) and adenosine 5′-monophosphate (AMP), as a growth-and production promoting ingredient, in culture media for culturing eukaryotic cells. The invention further pertains to culture media containing these components at levels of at least 0.001 mg / l.

The invention relates to a method for grafting mulberries in cold regions. The main problem influencing mulberry farming and silkworm rearing in northern areas is the mulberry breeding in winter. At present, grandifoliate mulberries which can survive below the temperature of 20 DEG are not available so silkworm breeding industries in the northern areas have larger restrictions, and tussahs are bred in only a few areas. The process of the invention comprises the following steps of: (1) breeding wild mulberry seeds in northeast cold regions, selecting and using well-developed seeding roots having no freezing injury and cold injury as cold resistance root stocks, and adopting mulberry shoots subjected to three times of winter training as scion to graft; (2) putting the grafted mulberry seeding into the environment with the temperature of 22-25 DEG temperature and the humidity of 75% to carry out seeding germination; (3) moving seeding stocks successfully germinated into fertilized nursery bases to plant, wherein three million seeding stocks are planted in the nursery base per mu by keeping the line spacing of 25 cm and the row spacing of 3-5 cm, and covering the seeding stocks by plastic films for 20-30 days; (4) and performing nursery base weeding and field management for about one year. The method can be used for breeding the mulberries with strong cold resistance.

The invention discloses a plastic profile all-steel type cooling water tank which comprises a tank body, a cover plate, inserting plates and water cooling shaping blocks. The inserting plates are vertically arranged in the tank body. Each water cooling shaping block is connected into the corresponding inserting plate in an inserted manner. The lengths of inserting grooves in the two sides of each inserting plate are different. An S-shaped water path is formed by the inserting plates. Equal-length stop blocks are arranged in the inserting grooves of each inserting plate. The stop blocks abut against the edges of the water cooling shaping blocks in the inserting grooves. Shaping holes in the water cooling shaping blocks are in one-to-one correspondence. According to the plastic profile all-steel type cooling water tank, heat expansion and cold contraction caused by the air temperature influence is avoided, and the sealing performance is high; the inner water path is designed to be the S circulation water flow direction, and therefore water flowability is increased; it is guaranteed that a plastic profile can be fast cooled and shaped, and the forming quality of the profile is improved; and meanwhile, the all-steel water tank is integrated, the sealing performance is high, a manufacturing process is simplified, water tank assembly errors are small, and the sealing performance is good. Resources are saved, cost is reduced, and therefore production is improved.

The invention discloses a maize transgenic tissue culture seedling transplanting method. The method comprises the following steps: (1) adding sterile water into tissue culture seedlings, and hardening the seedlings in a culture room for 1-2 days; (2) taking out and cleaning the seedlings, and soaking the parts from the roots to the parts, higher than the joints of the roots and the root stocks by 1-1.5 cm, of the seedlings in a solution containing thiophanate-methyl and copper calcium sulfate for 1 minute; (3) planting the tissue culture seedlings in nutrient soil prepared from humus soil, plant ash and vermiculite, adding thiophanate-methyl and copper calcium sulfate, adding bio-organic fertilizers, covering the seedlings with a film and standing for 2-3 days; and (4) respectively spraying an oxine-copper solution once from the fifth to seventh days and from the tenth to twelfth days after transplanting. According to the maize tissue culture seedling transplanting method provided in the invention, the transplanting survival rate of the maize tissue culture seedlings is improved to be over 97 percent, transplanted maize plants are vigorous in growth and can normally blossom and fruit, and the 25-40 fruits grow on each plant averagely. Moreover, the nutrient soil preparation and a sterilization method are improved, and the economic and manual cost is saved.

The invention provides a preparation method of a cultigen culture medium by taking unhusked rice as raw material. The preparation method includes the steps of material sorting, soaking, cleaning, mixing, bottling and sterilizing, inoculating and cultivating. The culture medium of edible fungus cultigen, which is prepared by taking the unhusked rice as the raw material, neither gives birth to bugs nor mildew, and has strong ability of resisting sundry fungus. After the culture medium of edible fungus cultigen is inoculated, the hypha buds quickly and grows strong with the rate of survival reaching 100%, and the strain can be preserved for a long period of two months.