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39results about How to "Strong production" patented technology

HMG2 promoter expression system and post-harvest production of gene products in plants and plant cell cultures

The invention relates in part to plant HMG2 HMGR genes and in part to the "post-harvest" production method of producing gene product of interest in plant tissues and cultures. The HMG2 promoter elements are responsive to pathogen-infection, pest-infestation, wounding, or elicitor or chemical treatments. The HMG2 elements are also active in specialized tissues of the plant including pollen and mature fruits. HMG2 promoter elements and HMG2-derived promoters can be advantageously used to drive the expression of disease and pest resistance genes, whereby transgenic plants containing such gene constructs would be resistant to the targeted disease and pest. In particular, the HMG2 gene expression system can be utilized in developing nematode resistant plants. The post-harvest production method of the invention utilizes plant tissues and cell cultures of plants or plant cells engineered with a expression construct comprising an inducible promoter, such as the HMG2 promoter, operably linked to a gene of interest. Production of the desired gene product is obtained by harvesting, followed by inducing and processing the harvested tissue or culture. The post-harvest production method may be advantageously used to produce direct or indirect gene products that are labile, volatile, toxic, hazardous, etc.
Owner:VIRGINIA TECH INTPROP INC

Cultivation method for iodine-containing spinach with high survival rate

The invention discloses a cultivation method for iodine-containing spinach with high survival rate. The cultivation method for iodine-containing spinach with high survival rate is characterized in that the cultivation method comprises the following steps: (1) sprouting the spinach seed; (2) fertilizing the solid iodine organic fertilizer into the humus soil surface, adding the smashed maize straw, plant ash, shale ash for forming the nutrition medium; (3) seedling in the nutrition medium; (4) transplanting the sprout to the shade; (5) fertilizing the compound fertilizer comprising 7-8% of long-lasting fertilizer additive. The cultivation method for iodine-containing spinach with high survival rate has the advantages that the seed quality is raised, the plant diseases and insect pests can be prevented and treated, the seed dormancy is destroyed, the seed is promoted for evenly sprouting and the seedling is promoted for growing strongly. The iodine fertilizer is extracted from the kelp, so that the iodine can be easily absorbed by the crops, the fertilizing method is simple and convenient and the persistent effect is good. The compound fertilizer comprising long-lasting fertilizer additive is used for raising the effectiveness of the soil phosphate. The transplanting survival rate of the method is high, the transplanted plant can grow strongly and remarkable economic benefit and social benefit can be achieved.
Owner:QINGDAO XINRUNTU NURSERY STOCK SPECIALIZED COOP

Broom and manufacturing method thereof

The invention discloses a broom and a manufacturing method thereof. The broom of the invention comprises a broom head and broom strips fixed on the bottom end of the broom head, wherein, the broom strips can be coconut palm fibre or cocoa fibre. The bottom end of the broom head is provided with a fixed groove in the shape of straight line and equal sign or the shape of Chinese character of hui, and the broom strips are integrally stuck in the fixed groove. The manufacturing method of the invention comprises the following steps: 1) the bottom end surface of the broom head is provided with the fixed groove with proper depth; 2) the connecting end of bundles of broom strips is dipped with glue to be consolidated into blocks; 3) bundles of broom strips which are consolidated into blocks are sliced into pieces according to the width of the fixed groove; 4) glue is filled into the fixed groove; 5) the connecting end of the broom strips which are sliced into pieces is inserted into the fixed groove to ensure that the connecting end and the fixed groove are fixedly connected. The broom of the invention has favourable tiny dust adsorbing effect, through cleaning, simple production, high efficiency, hardiness and low cost and is suitable for manual production, especially for manufacturing by families and small factories.
Owner:广州市日有日用品有限公司

Production method for strawberry seedling propagation and celery crop rotation in northern area

The invention discloses a production method for strawberry seedling propagation and celery crop rotation in a northern area and solves the problems of high seedling propagation cost, unstable seedlingquality and low efficiency existing in existing strawberry production. The production method comprises the steps: 1, cultivating strawberry mother plants, namely 1) preparing a strawberry mother plant culture medium; 2) selecting virus-free robust strawberry plants as mother plants in early April, and carrying out field planting on the virus-free robust strawberry plants in the mother plant culture medium; and 3) recovering the mother plants, and then, additionally applying a nitrogen-phosphorus-potassium balanced fertilizer once every other 15 days according to the growth vigor of the plants; 2, acquiring creeping stem seedlings, and preparing for celery germination acceleration and seedling culture, namely 1) acquiring the creeping stem seedlings; and 2) carrying out celery germinationacceleration and seedling culture, namely starting celery seedling culture work when creeping stems of the strawberry mother plants sprout; 3, cultivating robust strawberry seedlings; 4, harvesting the strawberry seedlings, and carrying out field planting on celeries; 5, cultivating the strawberries in a greenhouse; and 6, carrying out harvesting, namely, harvesting fresh strawberries to make thefresh strawberries appear on the market from the new year to the spring festival, and harvesting the celeries in good time before or after the new year according to market demands.
Owner:JILIN AGRICULTURAL UNIV

Method for grafting mulberries in cold region

The invention relates to a method for grafting mulberries in cold regions. The main problem influencing mulberry farming and silkworm rearing in northern areas is the mulberry breeding in winter. At present, grandifoliate mulberries which can survive below the temperature of 20 DEG are not available so silkworm breeding industries in the northern areas have larger restrictions, and tussahs are bred in only a few areas. The process of the invention comprises the following steps of: (1) breeding wild mulberry seeds in northeast cold regions, selecting and using well-developed seeding roots having no freezing injury and cold injury as cold resistance root stocks, and adopting mulberry shoots subjected to three times of winter training as scion to graft; (2) putting the grafted mulberry seeding into the environment with the temperature of 22-25 DEG temperature and the humidity of 75% to carry out seeding germination; (3) moving seeding stocks successfully germinated into fertilized nursery bases to plant, wherein three million seeding stocks are planted in the nursery base per mu by keeping the line spacing of 25 cm and the row spacing of 3-5 cm, and covering the seeding stocks by plastic films for 20-30 days; (4) and performing nursery base weeding and field management for about one year. The method can be used for breeding the mulberries with strong cold resistance.
Owner:吴庭贵

A plastic profiled all-steel cooling water tank

The invention discloses a plastic profile all-steel type cooling water tank which comprises a tank body, a cover plate, inserting plates and water cooling shaping blocks. The inserting plates are vertically arranged in the tank body. Each water cooling shaping block is connected into the corresponding inserting plate in an inserted manner. The lengths of inserting grooves in the two sides of each inserting plate are different. An S-shaped water path is formed by the inserting plates. Equal-length stop blocks are arranged in the inserting grooves of each inserting plate. The stop blocks abut against the edges of the water cooling shaping blocks in the inserting grooves. Shaping holes in the water cooling shaping blocks are in one-to-one correspondence. According to the plastic profile all-steel type cooling water tank, heat expansion and cold contraction caused by the air temperature influence is avoided, and the sealing performance is high; the inner water path is designed to be the S circulation water flow direction, and therefore water flowability is increased; it is guaranteed that a plastic profile can be fast cooled and shaped, and the forming quality of the profile is improved; and meanwhile, the all-steel water tank is integrated, the sealing performance is high, a manufacturing process is simplified, water tank assembly errors are small, and the sealing performance is good. Resources are saved, cost is reduced, and therefore production is improved.
Owner:ANHUI NAIKE EXTRUSION SCI & TECH

A kind of transplanting method of corn transgenic tissue culture seedling

The invention discloses a maize transgenic tissue culture seedling transplanting method. The method comprises the following steps: (1) adding sterile water into tissue culture seedlings, and hardening the seedlings in a culture room for 1-2 days; (2) taking out and cleaning the seedlings, and soaking the parts from the roots to the parts, higher than the joints of the roots and the root stocks by 1-1.5 cm, of the seedlings in a solution containing thiophanate-methyl and copper calcium sulfate for 1 minute; (3) planting the tissue culture seedlings in nutrient soil prepared from humus soil, plant ash and vermiculite, adding thiophanate-methyl and copper calcium sulfate, adding bio-organic fertilizers, covering the seedlings with a film and standing for 2-3 days; and (4) respectively spraying an oxine-copper solution once from the fifth to seventh days and from the tenth to twelfth days after transplanting. According to the maize tissue culture seedling transplanting method provided in the invention, the transplanting survival rate of the maize tissue culture seedlings is improved to be over 97 percent, transplanted maize plants are vigorous in growth and can normally blossom and fruit, and the 25-40 fruits grow on each plant averagely. Moreover, the nutrient soil preparation and a sterilization method are improved, and the economic and manual cost is saved.
Owner:BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI

Edible fungus culture medium containing astragalus membranaceus stems and leaves and using method

The invention provides an edible fungus culture medium containing astragalus membranaceus stems and leaves and a using method. The edible fungus culture medium is prepared from the following components in parts by weight: 60-100 parts of astragalus membranaceus stems and leaves, 75 parts of corncobs, 20 parts of bran, 2 parts of gypsum powder, 1 part of soybean flour, 1 part of calcium superphosphate and 1 part of white sugar. The using method comprises the following steps that after autumn astragalus membranaceus medicinal materials are harvested, stems and leaves on the ground are collected, cleaned, aired, crushed and uniformly mixed with other components in proportion, and material covering, bagging, sterilizing and cooling are carried out to obtain the edible fungus culture medium containing the astragalus membranaceus stems and leaves. A common edible mushroom culture medium is scientifically optimized, the ratio of astragalus membranaceus stems and leaves is strictly tested and can be applied to production of common edible fungus in the market, and resource utilization of agricultural production waste of astragalus membranaceus is achieved. The edible fungus culture medium containing the astragalus membranaceus stems and leaves and the using method have the advantages of low cost, yield increase, period shortening, safety and environmental protection, and has wide market space and application prospect.
Owner:INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI

A high-yield cultivation device with an annual output of 3‑4 crops of low-carbon energy raw materials

ActiveCN104488596BThe temperature of the growing environment is stableStable temperatureClimate change adaptationGreenhouse cultivationGreenhouseEnvironmental engineering
The invention provides a cultivation device for low-carbon energy raw materials with a high annual yield of 3 to 4 crops. The device comprises a sunlight heated greenhouse and a skeleton thereof, wherein the sunlight heated greenhouse is provided with a space heating unit, a soil heating unit and a multifunctional auxiliary unit; the space heating unit is composed of a heat-supplying heating pipe, a circulating pump 1, a circulating pump 2, a waste water pool and a natural hot water pool; the soil heating unit is composed of a heat-supplying heating pipe, a circulating pump 3, a circulating pump 4, the waste water pool and the natural hot water pool; and the multifunctional auxiliary unit is composed of a spray water tank and spay pipes, wherein the spray water tank is arranged out of the sunlight heated greenhouse, the spay pipes with spay heads and spray pumps are installed in and out of the sunlight heated greenhouse, one ends of the spray pipes are connected with the spay water tank, and the other ends of the spray pipes extend into the inner space of the sunlight heated greenhouse and are arranged fully in a parallel plane with a height of 2 m in a straight-line arrangement way or arranged fully in a vertical plane with the horizontal height as reference in any way.
Owner:贺先文

Broom and manufacturing method thereof

The invention discloses a broom and a manufacturing method thereof. The broom of the invention comprises a broom head and broom strips fixed on the bottom end of the broom head, wherein, the broom strips can be coconut palm fibre or cocoa fibre. The bottom end of the broom head is provided with a fixed groove in the shape of straight line and equal sign or the shape of Chinese character of hui, and the broom strips are integrally stuck in the fixed groove. The manufacturing method of the invention comprises the following steps: 1) the bottom end surface of the broom head is provided with the fixed groove with proper depth; 2) the connecting end of bundles of broom strips is dipped with glue to be consolidated into blocks; 3) bundles of broom strips which are consolidated into blocks are sliced into pieces according to the width of the fixed groove; 4) glue is filled into the fixed groove; 5) the connecting end of the broom strips which are sliced into pieces is inserted into the fixedgroove to ensure that the connecting end and the fixed groove are fixedly connected. The broom of the invention has favourable tiny dust adsorbing effect, through cleaning, simple production, high efficiency, hardiness and low cost and is suitable for manual production, especially for manufacturing by families and small factories.
Owner:广州市日有日用品有限公司
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