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Preparation method of cultigen culture medium by taking unhusked rice as raw material

A culture medium and medium technology, applied in the field of preparation of edible fungus culture medium, can solve the problems of easy mildew, easy to absorb water, swelling, insects, etc. The effect that the ability of bacteria is strong

Inactive Publication Date: 2013-01-09
安徽天都灵芝制品公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to improve the quality and yield of edible fungi, wheat, corn, and cotton husks are mostly used as the medium for the production of edible fungus original species or cultivated species, although wheat grains, corn, and cotton husks have more complete nutrients than wood chips and rice bran. , but because it is easy to absorb water and swell, it is easy to rot and worm; in addition, the culture medium made of these raw materials needs to be treated with insecticide and disinfection, and the production method is relatively complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for preparing a cultivar culture medium using paddy as raw material, comprising the following steps:

[0025] (1) Material selection: choose rice that is free from mildew, rot, insects, and cracks;

[0026] (2) Soaking: Soak the rice with 1% lime water by weight. When the temperature of the lime water is above 25°C, the soaking time is 48 hours. During the soaking process, the lime water should be changed twice;

[0027] (3) Cleaning and mixing materials: pick up the soaked rice, wash it and drain the excess water, then add 1.5% of the weight of the rice bran, and stir evenly to obtain the culture medium;

[0028] (4) Bottling and sterilization: Bottling the culture medium in (3), filling it to the shoulder of the bottle, and tying the neck of the bottle; then placing the bottle in a pressure cooker for heat sterilization. The pressure is 0.5-1.5kg / m 2 , The sterilization time is 2.5 hours. That is, when the pressure gauge pointer of the pressure cooker rise...

Embodiment 2

[0033] A method for preparing a cultivar culture medium using paddy as raw material, comprising the following steps:

[0034] (1) Material selection: choose rice that is free from mildew, rot, insects, and cracks;

[0035] (2) Soaking: Soak the rice with 1% lime water by weight. When the temperature of the lime water is below 25°C, the soaking time is 72 hours. During the soaking process, the lime water should be changed 3 times;

[0036] (3) Cleaning and mixing materials: pick up the soaked rice and wash it, drain off excess water, then add bran accounting for 3% of the weight of the rice, and stir evenly to obtain a culture medium;

[0037] (4) Bottling and sterilization: bottle the culture medium in (3), put it on the shoulder of the bottle, and tie the bottle; then place the bottle in an atmospheric pressure cooker and heat and sterilize it for 8 hours;

[0038] (5) Inoculation: first sterilize the inoculation box with aerosol, and then place the bottle sterilized in (4) ...

Embodiment 3

[0041] The cultivar culture medium with rice prepared by the method of the present invention is compared with the existing culture medium with cottonseed hulls as raw material to cultivate mycelium, and its specific differences are as follows:

[0042] project Culture medium of the present invention Cottonseed hull medium Insects and mildew in the culture medium No mildew and moth-eaten phenomena Mildew and moth-eaten occur from time to time Germination of cultured mycelia Mycelium germinates quickly, about 15 days from inoculation to full growth The germination of the bacteria is slightly slower, about 25-30 days from inoculation to full growth Appearance of cultured hyphae Mycelium grows white and strong, even without yellow water The hyphae are dark, uniform in thickness, relatively thin, and yellow water appears Mycelium survival rate 100% 98% Culture storage time Bacteria can be stored for about 2 months Bacteria can b...

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PUM

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Abstract

The invention provides a preparation method of a cultigen culture medium by taking unhusked rice as raw material. The preparation method includes the steps of material sorting, soaking, cleaning, mixing, bottling and sterilizing, inoculating and cultivating. The culture medium of edible fungus cultigen, which is prepared by taking the unhusked rice as the raw material, neither gives birth to bugs nor mildew, and has strong ability of resisting sundry fungus. After the culture medium of edible fungus cultigen is inoculated, the hypha buds quickly and grows strong with the rate of survival reaching 100%, and the strain can be preserved for a long period of two months.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and relates to a culture medium of edible fungi, in particular to a preparation method of a culture medium of edible fungi using rice as a raw material. [0002] Background technique [0003] The culture medium of the original species and cultivated species of edible fungi is generally prepared from different raw materials according to the different strains, but basically the culture medium of saprophytic fungi such as mushrooms and straw mushrooms is prepared with raw materials such as manure and forage; wood rot Bacteria such as shiitake mushrooms, oyster mushrooms, black fungus, white fungus, Ganoderma lucidum, Hericium erinaceus, Poria cocos, etc. can be used as a culture medium for bacteria such as sawdust, rice bran, seed wood or cotton shells. [0004] In order to improve the quality and yield of edible fungi, wheat, corn, and cotton husks are mostly used as the medium f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 丁伦保
Owner 安徽天都灵芝制品公司
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