Transplanting method for peanut tissue cultured seedlings and nutrient soil

A technology for tissue culture seedlings and nutrient soil, applied in planting methods, horticultural methods, botanical equipment and methods, etc., can solve the problems of high tissue culture cost, low transplant survival rate, etc., and achieves improved transplant survival rate, significantly The effect of economic and social benefits

Inactive Publication Date: 2011-10-19
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of the deficiencies in the prior art, the first purpose of the present invention is to provide a method for transplanting peanut tissue cultured seedlings by improving the traditional peanut tissue culture technology, so as to overcome the problem of transplanting peanut tissue cultured seedlings in the prior art. Problems of low survival rate and high cost of tissue culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The transplanting of embodiment 1 peanut tissue culture seedling

[0016] Before the peanut tissue culture seedlings are transplanted, harden the Huayu No. 23 tissue culture seedlings in the culture bottle, take the culture bottle from the culture room to the field, and then remove the seal of the culture bottle. Add 2 mm sterile water to maintain humidity and temperature, replace the sterile water every 2 days, and harden the seedlings at room temperature for 6 days. While hardening the seedlings, mix the vegetable garden soil, humus soil, plant ash, and sieved river sand evenly according to the ratio of 1:1:1:1 by mass, sterilize them in an autoclave, and then use 0.01% mass ratio Add 50% carbendazim wettable powder and mix evenly, then put the nutrient soil into a 11cm×11cm soft plastic nutrient bowl. When transplanting, first take out the tissue culture seedlings from the culture bottle, carefully operate with tweezers, do not damage the root system, and then rinse...

Embodiment 2

[0017] The transplanting of embodiment 2 peanut tissue culture seedlings

[0018] Before transplanting the peanut tissue culture seedlings, harden the Huayu No. 30 tissue culture seedlings in the culture bottle, take the culture bottle from the culture room to the field, and then remove the seal of the culture bottle. Add 3 mm of sterile water to maintain humidity and temperature, replace the sterile water every 2 days, and harden the seedlings at room temperature for 7 days. While hardening the seedlings, mix the vegetable garden soil, humus soil, plant ash, and sieved river sand evenly according to the ratio of 1:1:1:1 by mass, sterilize them in an autoclave, and then use 0.01% mass ratio Add 50% carbendazim wettable powder and mix evenly, then put the nutrient soil into a 15cm×15cm soft plastic nutrient bowl. When transplanting, first take out the tissue culture seedlings from the culture bottle, carefully operate with tweezers, do not damage the root system, and then rins...

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Abstract

The invention discloses a transplanting method for peanut tissue cultured seedlings and nutrient soil. The transplanting method comprises the steps of seedling adaptation before transplanting, preparation of transplanting nutrient soil, selection of transplanting containers, reasonable transplanting, maintenance management after transplanting and the like. Meanwhile, the invention also provides the nutrient soil for transplanting the peanut tissue cultured seedlings. By using the transplanting method for the peanut tissue cultured seedlings and the nutrient soil, the transplanting survival rate can reach over 95 percent, the transplanted peanut plants are robust and can normally flower and fruit, and average 7 to 13 fruits are grown in each plant. The bottleneck problem of low survival rate of the peanut tissue cultured seedlings is solved, and a foundation is laid for the development of peanut tissue culture.

Description

technical field [0001] The invention relates to a method for transplanting peanut tissue culture seedlings and a nutrient soil used for transplanting peanut tissue culture seedlings, belonging to the technical field of peanut tissue culture. Background technique [0002] Peanut is an important oil crop, economic crop and edible crop in my country. The annual peanut planting area in China is more than 65 million mu, the average yield per mu is about 225 kg, and the total output is about 14 million tons. my country's research on peanut breeding, cultivation, plant protection and processing is at an international leading level. However, due to the narrow genetic basis of peanut, poor stress resistance, and susceptibility to various diseases and insect pests, the rooting rate of peanut tissue culture regenerated seedlings is low. Can take root, and its transplanting survival rate is also very low, especially the regenerated peanut seedlings with excellent traits obtained through ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01C14/00A01H4/00
Inventor 杨庆利禹山林朱凤陈明娜迟晓元杨珍潘丽娟陈娜
Owner SHANDONG PEANUT RES INST
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