A kind of transplanting method of corn transgenic tissue culture seedling

Abstract

The invention discloses a maize transgenic tissue culture seedling transplanting method. The method comprises the following steps: (1) adding sterile water into tissue culture seedlings, and hardening the seedlings in a culture room for 1-2 days; (2) taking out and cleaning the seedlings, and soaking the parts from the roots to the parts, higher than the joints of the roots and the root stocks by 1-1.5 cm, of the seedlings in a solution containing thiophanate-methyl and copper calcium sulfate for 1 minute; (3) planting the tissue culture seedlings in nutrient soil prepared from humus soil, plant ash and vermiculite, adding thiophanate-methyl and copper calcium sulfate, adding bio-organic fertilizers, covering the seedlings with a film and standing for 2-3 days; and (4) respectively spraying an oxine-copper solution once from the fifth to seventh days and from the tenth to twelfth days after transplanting. According to the maize tissue culture seedling transplanting method provided in the invention, the transplanting survival rate of the maize tissue culture seedlings is improved to be over 97 percent, transplanted maize plants are vigorous in growth and can normally blossom and fruit, and the 25-40 fruits grow on each plant averagely. Moreover, the nutrient soil preparation and a sterilization method are improved, and the economic and manual cost is saved.

Description

technical field [0001] The invention relates to a transplanting method of maize transgenic tissue culture seedlings, belonging to the technical field of maize transgenic tissue culture. Background technique [0002] Corn is an important food crop and economic crop in China. In 2012, my country's corn planting area was 524 million mu, with an average yield of about 1,400 jin per mu, and the total output in 2012 was about 400 billion jin. my country's research on corn breeding, cultivation, plant protection, processing and other fields is at the international leading level. The development of modern transgenic technology has played an important role in improving maize resistance and improving maize quality, and maize transgenic breeding has gradually occupied a place in the field of breeding. Because the inbred lines selected for maize transgenic breeding are susceptible inbred lines, have poor stress resistance and are susceptible to various diseases and insect pests, and th...

AI Technical Summary

Problems solved by technology

[0003] The transplanting method of the existing corn transgenic tissue culture seedlings comprises the steps of hardening the seedlings, washing the culture medium with sterilized water, and transplanting to the finished nutrient soil sterilized by high pressure steam. This transplanting method has two disadvantages: 1. There are no measures to improve the stress resistance and adaptability of the tissue culture seedlings outside the short-term seedling hardening, and the survival rate of transgenic corn seedlings is slightly lower, about 60%-80%, with an average of 20-30 grains per plant; 2. The treatment of nutrient soil is high-pressure steam sterilization, which not only requires the purchase of sterilization equipment, but also manually packs, sterilizes, and air-dries the nutrient soil, which increases economic and labor costs