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Method using xylose production waste liquid to produce acetone and butanol

A technology of xylose mother liquor and xylose, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high production cost, complicated process, unstable quality, etc.

Active Publication Date: 2012-02-15
SHANDONG LONGLIVE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to provide a method for producing acetone and butanol from xylose production waste liquid, so as to solve the problems of complex process, low yield of acetone and butanol, unstable quality and high production cost in the prior art

Method used

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  • Method using xylose production waste liquid to produce acetone and butanol
  • Method using xylose production waste liquid to produce acetone and butanol
  • Method using xylose production waste liquid to produce acetone and butanol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] ATCC55025 strain and CICC8016 use xylose mother liquor medium to produce acetone butanol:

[0022] Preparation of Clostridium beijerinckii Clostridium beijerinckii ATCC55025. Transfer the above-mentioned strain inoculation to the anaerobic tube of glucose seed culture medium. After the transfer, put the anaerobic tube into a water bath at 100℃ and boil it, immediately cool to room temperature, fill it with nitrogen, and let stand anaerobic culture at 37℃16 ~18h is the fermented seed liquid.

[0023] The above-mentioned glucose seed medium refers to: 1L contains 3 g yeast extract, 10 g beef extract, 10 g tryptone, 5 g glucose, 1 g soluble starch, 5 g sodium chloride, and 3 g sodium acetate trihydrate. L-cysteine ​​0.5 g, sterilized at 115°C for 15 minutes.

[0024] Clostridium acetobutylicum Clostridium acetobutylicum CICC8016. Transfer the above strain inoculation to the anaerobic tube of 5% corn mash seed culture medium. After the transfer, put the anaerobic tube into a ...

Embodiment 2

[0031] The ATCC55025 strain uses xylose mother liquor and glucose to ferment to produce acetone and butanol:

[0032] Clostridium beijerinckii Clostridium beijerinckii ATCC55025 in the anaerobic flask, xylose mother liquor fermentation medium and glucose fermentation medium for acetone butanol fermentation comparison.

[0033] Using the seed liquid prepared in Example 1, according to the inoculum amount of 5%, it was transferred to a fermentation medium containing 50g / L glucose. After the transfer, it was filled with nitrogen and allowed to stand anaerobic culture at 37°C for more than 72 hours, which is acetone , Butanol fermentation product.

[0034] The above-mentioned glucose fermentation medium has the following ingredients: 5g glucose (the medium contains 48.3g / L glucose as determined by HPLC after sterilization), 0.1g yeast powder, KH 2 PO 4 0.05g, K 2 HPO 4 0.05g, ammonium acetate 0.22g, MgSO 4 ·7H 2 O 0.02g, MnSO 4 ·7H 2 O 0.001g, NaCl 0.001g, FeSO 4 ·7H 2 O 0.001g, dilut...

Embodiment 3

[0040] Different concentrations of xylose mother liquor is directly added with 0.2% yeast powder to ferment to produce acetone and butanol:

[0041] Prepare Clostridium beijerinckii as described in Example 1 Clostridium beijerinckii ATCC55025 seed liquid. That is, transfer the above-mentioned strain inoculation to the anaerobic tube of the glucose seed culture medium. After the transfer, put the anaerobic tube into a 100°C water bath to boil, immediately cool to room temperature, fill with nitrogen, and let stand anaerobic at 37°C. Cultured for 16h is the fermentation seed liquid.

[0042] Xylose mother liquor optimization optimization medium: configure 5 bottles of 100mL medium, in which the xylose mother liquor is directly diluted according to the concentration gradient of 6% (v / v), and 0.2g yeast powder is added respectively, the pH is natural, and it is connected to ATCC55025 The fermented seed liquid was fermented for 72 hours, and the content of acetone and butanol was dete...

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Abstract

A method using the xylose production waste liquid to produce acetone and butanol belongs to the technical field of biochemical engineering, utilizes the xylose production waste liquid as the raw material, utilizes the microbial fermentation technology and the biotransformation sugar source to produce chemical products of acetone and butanol, and has the specific technological steps of culture medium preparation, bacterial strain activation, inoculation and fermentation, so the products of ethanol, acetone and butanol are obtained. The invention has advanced technology and a low equipment input cost; the utilization problem of the xylose production waste liquid is solved, and the additional value is high; compared with chemical synthesis, fossil resources are also saved; meanwhile, compared with the production method which takes the grain crop as the fermentation raw material, the price of the raw material is low, the method does not compete with the human being for food, and does not compete with the grain forest for land; and the comprehensive utilization of the xylose production waste liquid is realized, the comprehensive cost of the xylose production is reduced, the utilization efficiency of the biomass resource is improved, the industrial by-products are reused, the environment pollution is less, the energy consumption is low, and the social benefit is obvious.

Description

Technical field [0001] The invention belongs to the technical field of biochemical engineering and relates to a production method for preparing acetone and butanol by microbial fermentation using xylose production waste liquid as raw material. Background technique [0002] Acetone is an excellent organic solvent and an important chemical raw material. It is widely used in chemicals, plastics, organic synthesis, paint and other industries. Currently, the largest consumer of acetone is solvent, accounting for 28% of its total consumption, followed by methacrylic acid. The esters MMA and bisphenol A account for 23% and 21% of total consumption respectively. Others are also used in the production of acetone cyanohydrin and isopropyl alcohol. Butanol (butanol, butyl alcohol and 1-butanol) is a four-carbon alcohol with the molecular formula C 4 H 9 OH, its molecular weight is 74.12Da. Butanol is a colorless liquid with a unique odor. Aspiration of butanol vapor can easily cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/28C12P7/16C12P7/08C12R1/145
Inventor 夏蕊蕊程少博方诩肖林覃树林万冬梅
Owner SHANDONG LONGLIVE BIO TECH CO LTD
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