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A technology of stevioside and sweetness, which is applied in the field of food additives and sweeteners, and can solve the problems of small sample size and difficult industrial production
Inactive Publication Date: 2010-04-07
NANJING NORMAL UNIVERSITY
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Problems solved by technology
However, due to the small amount of samples that can be processed by these methods including various chromatographic methods, it is difficult to realize industrial production
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[0035] Preparation of bacterial solution:
[0036] Bacillus megaterium J2 (Bacillus megaterium J2) CCTCC NO: M209201 was cultured in a conventional broth liquid medium at 37° C. and 220 rpm for 48 hours to obtain the bacterial liquid.
[0037] Preparation of bacterial powder:
[0038] It includes fermentation culture of bacteria, separation of fermentation liquid, emulsification of bacteria slime and vacuum freeze drying of emulsion. That is, Bacillus megaterium J2 (Bacillus megaterium J2) CCTCC NO: M209201 was cultured in the above liquid based on the bacteria liquid obtained after 48 hours of culture, centrifuged at 8000rpm and 5min to collect the bacteria to obtain the sludge, and the sludge was vacuum-frozen under the condition of a vacuum of 1-5pa After drying for 30 hours, the bacteria powder is obtained.
[0039] Degradation characteristics of SS and RA by degrading bacteria:
[0047] 0.5% (W / W), pH 5.0 stevioside solution (RA purity is about 85%), sterilized and cooled, then added 5% (W / W) Bacillus megaterium J2CCTCC NO: M209201 bacterial solution, cultured at 37°C for 72h, after After conversion, SS was mostly degraded, and RA content remained unchanged. After the transformation, the cells were removed by centrifugation at 5000rpm, and the supernatant was removed by flocculation and sedimentation, adsorbed and separated by macroporous resin, and the purified liquid was crystallized to obtain RA with a purity of about 98%.
Embodiment 2
[0049] 0.01% (W / W), pH 4.5 stevioside solution (RA purity is about 75%), add 2% (W / W) bacteria powder of Bacillus megaterium J2CCTCC NO: M209201, after culturing at 37°C for 36 hours, according to the conventional RA with a purity of about 90% was obtained by separation and purification.
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Abstract
Stevioside is a novel sweeter which is extracted from stevia leaves, and the stevioside in the stevia leaves mainly comprises two components of stevioside and rebaudioside A. The sweetness and mouthfeel of the rebaudioside A are both excellent, while the stevioside has certain lingering bitterness; and as the structure and nature of the rebaudioside A are approximate to the structure and nature of the stevioside, the separation of rebaudioside A and stevioside features high cost and great technical difficulty. In the invention, microorganisms are transformed for 20h-150h under the conditions that the concentration of the stevioside is 0.01%-5% (W/W), the temperature is 25-80 DEG C, the quantity of inverting enzyme or bacterial solution is 0.1%-10% (W/W), and the pH value is 2.0-7.0, and the stevioside contained in the rebaudioside A solution can be selectively degraded, thus obtaining high purity rebaudioside A containing no stevioside. The biotransformation method used in the invention has temperate conditions, does not use toxic methanol and other chemicals or macroporous resin with high cost, thus being a novel technology with safety, greenness and environmental friendliness.
Description
technical field [0001] The invention relates to a method for separating and purifying a high-quality sweetener component-rebaudioside A or other components in other steviosides by using microorganisms and enzymatic methods, and belongs to the application field of food additives and sweeteners. Background technique [0002] Steviol glycoside is a white, odorless powder with a melting point of 198-202°C, high temperature resistance, rapid moisture absorption in the air, slightly soluble in ethanol, stable to heat, acid and alkali, and can be heated in a solution in the range of pH 4-10 It does not change or brown at 120°C, and most of it is not metabolized in the human body. It is a non-fermentable sugar, so the heat generated in the body is extremely low. Steviol glycoside (purity above 90%) is a white powder, and subpure product (purity 50%) is light brown. It is easily soluble in water and can quickly absorb moisture in the air. The solubility at room temperature exceeds 4...
Claims
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