Method for improving sweetness of stevia sugar

A technology of stevioside and sweetness, which is applied in the field of food additives and sweeteners, and can solve the problems of small sample size and difficult industrial production
CN101691389AInactive Publication Date: 2010-04-07NANJING NORMAL UNIVERSITY

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING NORMAL UNIVERSITY
Publication Date
2010-04-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

Stevioside is a novel sweeter which is extracted from stevia leaves, and the stevioside in the stevia leaves mainly comprises two components of stevioside and rebaudioside A. The sweetness and mouthfeel of the rebaudioside A are both excellent, while the stevioside has certain lingering bitterness; and as the structure and nature of the rebaudioside A are approximate to the structure and nature of the stevioside, the separation of rebaudioside A and stevioside features high cost and great technical difficulty. In the invention, microorganisms are transformed for 20h-150h under the conditions that the concentration of the stevioside is 0.01%-5% (W / W), the temperature is 25-80 DEG C, the quantity of inverting enzyme or bacterial solution is 0.1%-10% (W / W), and the pH value is 2.0-7.0, and the stevioside contained in the rebaudioside A solution can be selectively degraded, thus obtaining high purity rebaudioside A containing no stevioside. The biotransformation method used in the invention has temperate conditions, does not use toxic methanol and other chemicals or macroporous resin with high cost, thus being a novel technology with safety, greenness and environmental friendliness.
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Description

technical field

[0001] The invention relates to a method for separating and purifying a high-quality sweetener component-rebaudioside A or other components in other steviosides by using microorganisms and enzymatic methods, and belongs to the application field of food additives and sweeteners. Background technique

[0002] Steviol glycoside is a white, odorless powder with a melting point of 198-202°C, high temperature resistance, rapid moisture absorption in the air, slightly soluble in ethanol, stable to heat, acid and alkali, and can be heated in a solution in the range of pH 4-10 It does not change or brown at 120°C, and most of it is not metabolized in the human body. It is a non-fermentable sugar, so the heat generated in the body is extremely low. Steviol glycoside (purity above 90%) is a white powder, and subpure product (purity 50%) is light brown. It is easily soluble in water and can quickly absorb moisture in the air. The solubility at room temperature exceeds 4...

Claims

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