Composite bacterial agent and biological method for treating flow-back fracturing fluid to obtain oil displacement active water

A technology of composite bacterial agent and flowback liquid, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of consumption of water treatment agents, water quality impact, and difficult treatment, and shorten the hydraulic retention time , save energy consumption, improve the effect of interfacial tension

Inactive Publication Date: 2011-01-05
DALIAN BITEOMICS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After these chemicals enter the water treatment system, they will seriously disrupt the normal operation of the production system, such as consuming a large amount of water treatment chemicals or reducing the treatment effect of water treatment chemicals, affecting the removal of oil and harmful ions in the water body, and affecting the pollution generated during the water treatment process. Mud sedimentation, serious pollution of filter materials, etc., and most of them have high salinity and contain harmful substances such as crude oil, polysaccharides and phenols, which have a great impact on local water quality, resulting in the water quality after treatment not reaching the level of injected water Water quality requirements will cause greater losses to oilfield production and long-term development
[0003] Due to the production characteristics o...

Method used

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  • Composite bacterial agent and biological method for treating flow-back fracturing fluid to obtain oil displacement active water
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  • Composite bacterial agent and biological method for treating flow-back fracturing fluid to obtain oil displacement active water

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Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1: the compounding of microbial bacterial agent

[0025] The bacteria growing in the formation water of the oil well were enriched and cultured, then streaked on the LB agar plate, cultured at 37°C for 16 hours, a single colony was picked, and after screening, a strain was obtained, which was identified as Rhodococcus equi and numbered BS001 ;

[0026] At the same time, commercially known bacterial strains were taken: Pseudomonas aeruginosa, T. crassa, Bacillus subtilis, Bacillus cereus, and Paecillin were numbered BS002, BP001, BS003, BC001, and BP002, respectively.

[0027] Configure seed liquid culture medium, formula is: peptone, 10g / L; Yeast powder, 5g / L; NaCl, 5g / L; Molasses, 1% (volume ratio); KH 2 PO 4 , 3g / L; K 2 HPO 4 , 1.5g / L; natural pH. Sterilize at 115°C for 15 minutes. The liquid volume is 100mL / 250mL bottle volume. Pick the above-mentioned 6 strains and single colonies from each bottle. The constant temperature shaker rotates at 150rpm a...

Embodiment 2

[0029] Embodiment 2: the validation of compound bacterial agent producing biosurfactant

[0030] The surface tension of the fermentation broth was measured with the JYW-200 automatic surface / interfacial tension meter of Chengde Dingsheng Testing Machine Testing Co., Ltd. at room temperature to measure the surface tension of the fermentation broth. Each measurement was repeated three times, and the average value was taken. The specific operation of the surface tension is as follows: take 30mL fermentation broth and centrifuge at 8000rpm for 10min, take the supernatant in a measuring cup, and measure the surface tension with a platinum ring.

[0031] The liquid fermentation medium is as follows (by weight ratio):

[0032] Guar Gum Broken Liquid Monosaccharide [β-mannanase, Bio-Thera Port (Dalian) Biotechnology Co., Ltd.] 5g, NH 4 NO 3 2.0g, K 2 HPO 4 ·3H 2 O 3.0g, NaH 2 PO 4 2H 2 O 10g, MgSO 4 ·7H 2 O0.2g, MnCl 2 4H 2 O 2ppm, yeast extract 0.2g, adjust pH=7.0 with Na...

Embodiment 3

[0040] Example 3: Validation of bacterial strains producing biopolymers using gel-breaking fluid

[0041] In the invention, the surface activity and the sugar content experiment of the prepared bioactive water are carried out; the medium screening uses the surface tension value of the fermented liquid as the screening index.

[0042] The liquid fermentation medium is as follows (by weight ratio):

[0043] Guar Gum Broken Liquid Monosaccharide [β-mannanase, Bio-Thera Port (Dalian) Biotechnology Co., Ltd.] 5g, NH 4 NO 3 2.0g, K 2 HPO 4 ·3H 2 O 3.0g, NaH 2 PO 4 2H 2 O 10g, MgSO 4 ·7H 2 O0.2g, MnCl 2 4H 2 O 2ppm, yeast extract 0.2g, adjust pH=7.0 with NaOH solution.

[0044] The above-mentioned culture is inserted into the seed solution of the compound bacterial agent in step 2 of the summary of the invention according to the inoculum size 5% (V / V), and the specific composition is: peptone, 10g / L; yeast powder, 5g / L; NaCl, 5g / L; Molasses, 1% (by volume); KH 2 PO 4 ,...

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Abstract

The invention discloses a composite bacterial agent and a biological method for treating flow-back fracturing fluid to obtain oil displacement active water, which relate to biotransformation and reutilization technology of fracturing flow-back fluid. In the method, the fracturing flow-back fluid is treated by a microbial fermentation method, and a residual organic substance harmful to environment in the flow-back fluid is transformed into biological active water with certain function. The composite bacterial agent and the method are applied to reinjection water of water injection wells, reinjection water for single-well soaking, reinjection water for oil field blockage removal, reinjection water for other purposes, water for medicament preparation in a technical process and the like. The composite bacterial agent comprises biosurfactant, organic acid, organic ester, organic ketone, biogas and other metabolites produced by microbial metabolism of the fracturing flow-back fluid.

Description

technical field [0001] The invention relates to a biotransformation and reuse technology of fracturing flowback fluid. It is characterized in that the fracturing flowback fluid is treated with the method of compound microbial fermentation, and the residual organic substances harmful to the environment in the flowback fluid are converted into biologically active water with certain functions. Its application scope is water injection well injection; Reinjection water for single well huff and puff; reinjection water for oil well plugging; other reinjection water; water for pharmaceutical preparation in process, etc. Its main components are: biosurfactants, organic acids, organic esters, organic ketones and other metabolites of biogas produced by fracturing flowback fluid through microbial metabolism. Background technique [0002] Fracturing flowback fluid is extremely harmful to the environment. Due to the complex and stable components of its internal pollutants, it is difficu...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C12N1/14C02F3/34C12R1/01C12R1/385C12R1/125C12R1/085C12R1/79
Inventor 梅晓丹付步飞刘滢赵静王平陈琛杨平杜国丰刘润海刘文静
Owner DALIAN BITEOMICS INC
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